Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,380,724 T→A 10.0% intergenic (‑112/+23) PP_t67 ← / ← PP_t66 tRNA‑Met/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,380,7240TA10.0% 103.4 / 3.8 40intergenic (‑112/+23)PP_t67/PP_t66tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (14/22);  new base A (2/2);  total (16/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.37e-01

AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTA  >  NC_002947/1380579‑1380869
                                                                                                                                                 |                                                                                                                                                  
aGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTaa                                                                                                                                                  <  7:178721/148‑1 (MQ=255)
   ccGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGT‑GTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                             <  5:40472/149‑1 (MQ=255)
    cgacgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTg                                                                                                                                             <  7:55706/149‑1 (MQ=255)
      acgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTTAACACAGTGGc                                                                                                                                           <  3:113181/149‑1 (MQ=255)
      acgaGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGc                                                                                                                                           <  1:175702/149‑1 (MQ=255)
         aGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑tt                                                                                                                                       <  4:156823/149‑1 (MQ=255)
             aCCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACa                                                                                                                                                <  5:76905/137‑1 (MQ=255)
             aCCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACa                                                                                                                                                >  6:76905/1‑137 (MQ=255)
              ccAGGCTGCTCCATCCCGAGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCtt                                                                                                                                  >  8:217884/1‑149 (MQ=255)
                aGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGa                                                                                                                                <  8:179732/149‑1 (MQ=255)
                       tCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAg                                                                                                                                               <  2:322175/128‑1 (MQ=255)
                       tCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAg                                                                                                                                               >  1:322175/1‑128 (MQ=255)
                        ccATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTgg                                                                                                                          <  7:105952/147‑1 (MQ=255)
                        ccATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTgg                                                                                                                          >  8:105952/1‑147 (MQ=255)
                             ccGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCgg                                                                                                                    <  7:167559/148‑1 (MQ=255)
                              cgcgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCgg                                                                                                                    >  6:307270/1‑147 (MQ=255)
                                cgTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGc                                                                                                                <  5:307270/149‑1 (MQ=255)
                                     gTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGAt                                                                                                           <  3:165851/149‑1 (MQ=255)
                                       ggggCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGTCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGAtt                                                                                                          <  7:217884/148‑1 (MQ=255)
                                                         tgtgGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCgg                                                                                        <  1:37521/148‑1 (MQ=255)
                                                             gTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTAt                                                                                   >  2:48202/1‑149 (MQ=255)
                                                                     gTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCcga                                                                           >  5:96485/1‑149 (MQ=255)
                                                                              tAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTAc                                                                   >  5:126865/1‑148 (MQ=255)
                                                                                      gATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGgctgc                                                           <  1:48202/148‑1 (MQ=255)
                                                                                                         gcgAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTgggg                                       <  6:126865/149‑1 (MQ=255)
                                                                                                               gCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTAcc                                                                  <  8:195916/116‑1 (MQ=255)
                                                                                                               gCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTAcc                                                                  >  7:195916/1‑116 (MQ=255)
                                                                                                                ctaaTCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTTCCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGCATATGACCCAGACGAGCAACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGAta                                 >  2:97408/4‑147 (MQ=17)
                                                                                                                 ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGccc                                                                             <  8:124923/103‑1 (MQ=255)
                                                                                                                 ttATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGccc                                                                             >  7:124923/1‑103 (MQ=255)
                                                                                                                             aaaGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGt                   >  1:13601/1‑149 (MQ=255)
                                                                                                                                  aaaaGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTtata              <  6:96485/149‑1 (MQ=255)
                                                                                                                                      ggCCACTGTTAACACAGTGGCCTTTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGt           >  7:184281/1‑149 (MQ=17)
                                                                                                                                      ggCCACTGTTAACACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGa                                   >  7:252530/1‑124 (MQ=255)
                                                                                                                                      ggCCACTGTTAACACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGa                                   <  8:252530/124‑1 (MQ=255)
                                                                                                                                         cACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTAc                             >  5:75132/1‑127 (MQ=255)
                                                                                                                                         cACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTAc                             <  6:75132/127‑1 (MQ=255)
                                                                                                                                              tgtTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTa  <  2:13601/149‑1 (MQ=255)
                                                                                                                                               gtTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAG‑CCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGt                   <  3:288555/130‑1 (MQ=255)
                                                                                                                                               gtTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAG‑CCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGt                   >  4:288555/1‑130 (MQ=255)
                                                                                                                                                ttAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACcgacg                                                                                                <  4:269999/53‑1 (MQ=35)
                                                                                                                                                ttAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACcgacg                                                                                                >  3:269999/1‑53 (MQ=35)
                                                                                                                                                 |                                                                                                                                                  
AGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGTGTGTGGTTTGTATGTCAAGCGCTAGGCTTTGATTTTTCGACGTTTTTTCGCGAATGCTTATCAGCAGGCAAAGAAAAAGGCCACTGTGTTAACAGTGGCC‑TTTTCTTGAATCTGGTTGCGGGAGCCGGATTTGAACCGACGACCTTCGGGTTATGAGCCCGACGAGCTACCAGGCTGCTCCATCCCGCGTCAGTGGGGCGGATTCTACAGCGTGTAGTTTATATGTCAAGCGCTA  >  NC_002947/1380579‑1380869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑