Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,381,590	G→T	5.6%	S114I (AGC→ATC)	dinB →	DNA polymerase IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,381,590	0	G	T	5.6%	233.9 / 3.5	72	S114I (AGC→ATC)	dinB	DNA polymerase IV
Reads supporting (aligned to +/- strand):  ref base G (31/37);  new base T (2/2);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.53e-01

CTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCTGGCCAAGATCGCCAGTGACTGGCGCAAACCCAATGGG  >  NC_002947/1381442‑1381735                                                                                                                                                     |                                                                                                                                                  cTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGCACGAAGCTTATCTGGATGTCAGCGACAg                                                                                                                                                   >  6:274931/1‑149 (MQ=255) cTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGACCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAg                                                                                                                                                   >  5:279125/1‑149 (MQ=255)    aaGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCa                                                                                                                                                <  6:279125/149‑1 (MQ=255)        tttGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGCTGTCGCTGACATCCAg                                                                                                                                               >  8:267263/1‑146 (MQ=255)        tttGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGCTGTCGCTGACATCCAg                                                                                                                                               <  7:267263/146‑1 (MQ=255)        tttGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTgg                                                                                                                                            >  2:279673/1‑149 (MQ=255)          tGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGCTGTCGCTGACATCCAgataa                                                                                                                                           <  4:271720/148‑5 (MQ=255)          tGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTgg                                                                                                                                            <  2:124529/147‑1 (MQ=255)           gCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTgg                                                                                                                                            <  6:265250/146‑1 (MQ=255)           gCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTag                                                                                                                                         >  5:265250/1‑148 (MQ=255)              ccGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCg                                                                                                                                      >  5:113357/1‑149 (MQ=255)                      ttGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCggg                                                                                                                                    <  3:47730/143‑1 (MQ=255)                      ttGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAgcgc                                                                                                                              >  6:253759/1‑149 (MQ=255)                      ttGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAgcg                                                                                                                               <  3:225079/148‑1 (MQ=255)                         aTCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTg                                                                                                                                             >  5:256295/1‑131 (MQ=255)                         aTCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTg                                                                                                                                             <  6:256295/131‑1 (MQ=255)                                 gCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTg                                                                                                                   >  4:274415/1‑149 (MQ=255)                                 gCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTg                                                                                                                   >  1:231824/1‑149 (MQ=255)                                   cgcgCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGcc                                                                                                                 >  7:220557/1‑149 (MQ=255)                                    gcgcTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGcc                                                                                                                 >  5:124895/1‑148 (MQ=255)                                    gcgcTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGcc                                                                                                                 >  8:45028/1‑148 (MQ=255)                                     cgcTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGa                                                                                                               <  1:212159/149‑1 (MQ=255)                                     cgcTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGa                                                                                                               <  1:279673/149‑1 (MQ=255)                                          cGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGa                                                                                                            <  7:267472/147‑1 (MQ=255)                                                cTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGcc                                                                                                    >  3:243797/1‑149 (MQ=255)                                                cTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGcc                                                                                                    >  5:294233/1‑149 (MQ=255)                                                        aGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGtt                                                                                            <  7:241892/149‑1 (MQ=255)                                                          gCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGc                                                                                          >  5:270104/1‑149 (MQ=255)                                                           ccTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGcc                                                                                         <  6:113357/149‑1 (MQ=255)                                                             tCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCAcg                                                                                                                          >  3:295396/1‑114 (MQ=255)                                                             tCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCAcg                                                                                                                          <  4:295396/114‑1 (MQ=255)                                                                cgGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGGCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCAc                                                                                    <  2:211486/149‑1 (MQ=255)                                                                 gggAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCAcc                                                                                   <  3:274415/149‑1 (MQ=255)                                                                   gAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCt                                                                                 >  7:42098/1‑149 (MQ=255)                                                                       tCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCa                                                                              >  4:236177/1‑148 (MQ=255)                                                                       tCCAAACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCat                                                                             <  5:110761/149‑1 (MQ=255)                                                                          acacGATCTTTCGCGATTACTCGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGc                                                                                                 <  1:276868/126‑1 (MQ=255)                                                                          acacGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGc                                                                                                 >  2:276868/1‑126 (MQ=255)                                                                          acacGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCatat                                                                           >  1:282519/1‑148 (MQ=255)                                                                          acacGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATc                                                                          <  6:270104/149‑1 (MQ=255)                                                                           cacGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCa                                                                         >  2:287685/1‑149 (MQ=255)                                                                             cGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCAcc                                                                       <  2:231824/149‑1 (MQ=255)                                                                                  tttCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGTATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTc                                                                  >  6:234809/1‑149 (MQ=255)                                                                                  tttCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTc                                                                  <  8:42098/149‑1 (MQ=255)                                                                                    tCGCGAGTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTc                                                                  <  5:253759/147‑1 (MQ=255)                                                                                      gcgATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCt                                                              >  5:188587/1‑149 (MQ=255)                                                                                             cacGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGACCGCACCCTGCATATCACCGTGTCGGCtggcgtgg                                                       >  7:80026/1‑149 (MQ=255)                                                                                              acGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCtggcgtggc                                                      >  3:297342/1‑149 (MQ=255)                                                                                                 gAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGcc                                                   <  6:294233/149‑1 (MQ=255)                                                                                                       aTCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCCCCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAAc                                               >  3:4849/1‑147 (MQ=255)                                                                                                       aTCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAAc                                               <  6:188587/147‑1 (MQ=255)                                                                                                        tCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCTCCGTGTCGGCtg                                                             <  8:23297/132‑1 (MQ=255)                                                                                                        tCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCtg                                                             >  7:23297/1‑132 (MQ=255)                                                                                                          gAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCTCCGTGTCGGCTGGCGTGGCGCCTAACAAGt                                           <  1:28291/148‑1 (MQ=255)                                                                                                             ccTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCGGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCTCCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCt                                       <  5:274931/149‑1 (MQ=255)                                                                                                             ccTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCt                                       >  1:120218/1‑149 (MQ=255)                                                                                                              cTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCTg                                      <  8:220557/149‑1 (MQ=255)                                                                                                                 tGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCTgg                                     <  6:124895/147‑1 (MQ=255)                                                                                                                      cTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCTGGcc                                   >  2:17707/1‑144 (MQ=255)                                                                                                                       tGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCTGGcc                                   >  1:79135/1‑143 (MQ=255)                                                                                                                           cGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGTATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCTGGCCAAGATCGCCa                         <  5:234809/149‑1 (MQ=255)                                                                                                                           cGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTGACAAGTTTCTGGCCAAGATCGCCa                         <  2:282519/149‑1 (MQ=255)                                                                                                                           cGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCTGGCCAAGATCGCCa                         <  1:287685/149‑1 (MQ=255)                                                                                                                             aaGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCTGGCCAAGATCGCCAGt                       <  2:79135/149‑1 (MQ=255)                                                                                                                                   aTCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCTGGCCAAGATCGCCAGTGAc                    <  3:236177/146‑1 (MQ=255)                                                                                                                                   aTCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCTGGCCAAGATCGCCAGTGACTg                  <  4:297342/148‑1 (MQ=255)                                                                                                                                        gATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACa                                              >  7:52118/1‑115 (MQ=255)                                                                                                                                        gATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACa                                              <  8:52118/115‑1 (MQ=255)                                                                                                                                        gATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCATGCATATCACCGTGTCGGCTGGCGTGGCGCCTAAAAAGTTTCTGGACAAGATCGACAGTGACTGgcgaa             >  6:329527/1‑146 (MQ=255)                                                                                                                                          tGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCTGGCCAAGATCGCCAGTGACTGGCGCAAAc          <  4:243797/149‑1 (MQ=255)                                                                                                                                             cAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCTGGCCAAGATCGCCAGTGACTGgcgc              <  2:258661/142‑1 (MQ=255)                                                                                                                                             cAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCTGGCCAAGATCGCCAGTGACTGgcgc              >  1:258661/1‑142 (MQ=255)                                                                                                                                              gctgACATCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCTGGCCAAGATCGCCAGTGACTGGCGCAAACCCaa      >  3:126991/4‑149 (MQ=255)                                                                                                                                                  aCAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCTGGCCAAGATCGCCAGTGACTGGCGCAAACCCAATggg  <  2:120218/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CTGAAGCTTTGCCCCGATCTGTTGATCGTCAAGCCGCGCTTCGAGGCCTACCGTGAGGCCTCGCGGGAAATCCACACGATCTTTCGCGATTACACGGAGCTCATCGAGCCTTTGTCCCTGGACGAAGCTTATCTGGATGTCAGCGACAGCCAGTGGTATTCGGGCAGCGCCACGCGCATTGCCGAGGATATTCGCCGGCGTGTTGCCCGCACCCTGCATATCACCGTGTCGGCTGGCGTGGCGCCTAACAAGTTTCTGGCCAAGATCGCCAGTGACTGGCGCAAACCCAATGGG  >  NC_002947/1381442‑1381735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑