Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,383,070 T→G 7.9% E77D (GAA→GAC)  PP_1204 ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,383,0700TG 7.9% 147.1 / 4.6 51E77D (GAA→GAC) PP_1204lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (22/25);  new base G (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

TACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATC  >  NC_002947/1382922‑1383217
                                                                                                                                                    |                                                                                                                                                     
tACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGCCCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCCGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAAt                                                                                                                                                       >  5:190091/1‑149 (MQ=255)
   tttGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCg                                                                                                                                                    >  4:37095/1‑149 (MQ=255)
   tttGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAAGTca                                                                                                                                                    >  7:263281/1‑148 (MQ=255)
     tGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCg                                                                                                                                                    <  3:107141/147‑1 (MQ=255)
      gCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGt                                                                                                                                                  <  2:73262/148‑1 (MQ=255)
            ggTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCg                                                                                                                                           <  2:294625/149‑1 (MQ=255)
                 cccAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAAGTCACCGAATTCGAAgt                                                                                                                                       <  8:263281/148‑2 (MQ=255)
                  ccAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCTAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAgcgc                                                                                                                                     >  4:101429/1‑149 (MQ=255)
                  ccAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAgcgc                                                                                                                                     >  4:184631/1‑149 (MQ=255)
                          aaGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTc                                                                                                                             <  3:184631/149‑1 (MQ=255)
                            gACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCAt                                                                                                                           >  8:261211/1‑149 (MQ=255)
                                           ggCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTc                                                                                                            >  3:247015/1‑149 (MQ=255)
                                                    acCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCgg                                                                                                     >  4:176226/1‑147 (MQ=255)
                                                        gCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTc                                                                                             <  6:44881/149‑1 (MQ=255)
                                                         ccagcaCGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTcc                                                                                            <  6:190091/149‑1 (MQ=255)
                                                         ccaccaCGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTcc                                                                                            <  3:37095/149‑1 (MQ=255)
                                                         ccaccaCGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTc                                                                                             >  1:220665/1‑148 (MQ=255)
                                                          caccacGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTg                                                                                                                                <  8:104605/114‑1 (MQ=255)
                                                          caccacGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTg                                                                                                                                >  7:104605/1‑114 (MQ=255)
                                                             cacGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCa                                                                                        >  3:154335/1‑149 (MQ=255)
                                                             cacGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCa                                                                                        >  4:285525/1‑149 (MQ=255)
                                                              acGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCAc                                                                                       <  7:261211/149‑1 (MQ=255)
                                                               cGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCAcc                                                                                      <  4:287863/149‑1 (MQ=255)
                                                                 gCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCtt                                                                                    >  4:20190/1‑149 (MQ=255)
                                                                  gcACTGCACCCAGCGGGATCTCGGTGATCTCCCGCCATGGTTTGTAAGGCGGGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTc                                                                                   <  8:153789/148‑1 (MQ=255)
                                                                        cACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTc                                                                              <  4:69601/148‑1 (MQ=255)
                                                                                       cGTAGATCTCCCGCCAGGCTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTCACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTg                                                               >  6:109809/1‑148 (MQ=255)
                                                                                                cccGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGc                                                     >  4:222067/1‑149 (MQ=255)
                                                                                                 ccGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCt                                                    >  2:225597/1‑149 (MQ=255)
                                                                                                    ccAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTc                                                   >  5:308457/1‑147 (MQ=255)
                                                                                                     cgttcttGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGa                                                 <  7:179675/143‑1 (MQ=255)
                                                                                                     aaGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAg                                                <  4:247015/148‑1 (MQ=255)
                                                                                                          ttGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGAACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGtt                                           >  3:105031/1‑149 (MQ=255)
                                                                                                            ggTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGa                                                 >  8:179675/1‑141 (MQ=255)
                                                                                                             gTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTgc                                        <  6:308457/149‑1 (MQ=255)
                                                                                                              tAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGTACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTgcg                                       <  4:105031/149‑1 (MQ=255)
                                                                                                                     tgtAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCTCTCACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGAcg                                <  5:330065/149‑1 (MQ=255)
                                                                                                                        aGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATc                                                                                         >  5:88139/1‑89 (MQ=255)
                                                                                                                        aGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATc                                                                                         <  6:88139/89‑1 (MQ=255)
                                                                                                                             tCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGctcctc                        <  1:225597/149‑1 (MQ=255)
                                                                                                                               aTAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCAt                      >  8:121320/1‑149 (MQ=255)
                                                                                                                                    gacgGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCATGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTcg                 <  2:220665/149‑1 (MQ=255)
                                                                                                                                      aaaagcgcTTCGAAGTCACCGAATTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTcgct               >  5:238980/8‑149 (MQ=255)
                                                                                                                                         gTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTcgctgcg            >  7:154411/1‑149 (MQ=255)
                                                                                                                                            aCTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTcgctgcgctg         <  8:154411/149‑1 (MQ=255)
                                                                                                                                            aCTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTcgctgcgctg         <  3:285525/149‑1 (MQ=255)
                                                                                                                                             cTTCGAAGTCACCGAATTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTgg        <  6:238980/149‑1 (MQ=255)
                                                                                                                                              ttCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGGGATCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTcgctgcgctg         >  1:253920/1‑149 (MQ=255)
                                                                                                                                                cGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTggg       <  2:122631/147‑1 (MQ=255)
                                                                                                                                                   aTTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACAt   <  7:121320/148‑1 (MQ=255)
                                                                                                                                                   aTTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATc  <  3:20190/149‑1 (MQ=255)
                                                                                                                                                   aTTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATc  >  2:159767/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TACTTTGCGGCAGGTTACCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAA‑‑GCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATC  >  NC_002947/1382922‑1383217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑