| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 13,279 | A→G | 6.6% | H353R (CAC→CGC) | recF → | DNA replication/repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 13,279 | 0 | A | G | 6.6% | 179.2 / 2.6 | 62 | H353R (CAC→CGC) | recF | DNA replication/repair protein |
| Reads supporting (aligned to +/- strand): ref base A (31/26); new base G (2/2); total (34/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
ATGACTTGCCGTCCGAACTGGATGACCAGCATCGCCGCGCACTGTGCCGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGCGTCCCGGCATGTACATTGGCGACACC > NC_002947/13132‑13425 | aTGACTTGCCGTCCGAACTGGATGACCAGCATCGCCGCGCACTGTGCCGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCAc > 6:204768/1‑149 (MQ=255) cTTGCCGTCCGAACTGGATGACCAGCATCGCCGCGCACTGTGCCGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTgg < 3:262037/149‑1 (MQ=255) ccGTCCGAACTGGATGACCAGCATCGCCGCGCACTGTGCCGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAAc < 5:204768/148‑1 (MQ=255) gTCCGAACTGGATGACCAGCATCGCCGCGCACTGTGCCGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACTCCAGTTGC‑TTTGTTCCACGTGGAACaaa < 1:14282/149‑2 (MQ=255) gTCCGAACTGGATGACCAGCATCGCCGCGCACTGTGCCGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACTCCAGTTGC‑TTTGTTCCACGTGGAACaaa < 1:19447/149‑2 (MQ=255) cGAACTGGATGACCAGCATCGCCGCGCACTGTGCCGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGcc < 3:186942/149‑1 (MQ=255) gATGACCAGCATCGCCGCGCACTGTGCCGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACG‑CAGTTGCATTTTTTCACCGGGGAACAGGGCGGTAGCAc > 1:68773/1‑149 (MQ=255) tCGCCGCGCACTGTGCCGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACTGAAACGCCAGTTGC‑TTTGTTCCGCGTGGAACaaa > 6:269146/1‑127 (MQ=255) tCGCCGCGCACTGTGCCGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCGCGTGGAACaaa > 6:26804/1‑127 (MQ=255) tCGCCGCGCACTGTGCCGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCGCGTGGAACaaa < 5:269146/128‑2 (MQ=255) tCGCCGCGCACTGTGCCGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCGCGTGGAACaaa < 5:26804/128‑2 (MQ=255) tCGCCGCGCACTGTGCCGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACg > 1:281119/1‑149 (MQ=255) gcgcACTGTGCCGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTCAGTGAAGTATGGAAGAAGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGCCCCCCGACCAt > 8:148783/1‑149 (MQ=255) tgCCGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGt < 5:185920/148‑1 (MQ=255) ccGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGaa < 2:84025/149‑1 (MQ=255) ccGCTTGCTTGAACAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGaa < 5:323256/149‑1 (MQ=255) gcttgcttGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCGGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCgg < 2:44392/140‑1 (MQ=255) gcttgcttGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCGGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCgg > 1:44392/1‑140 (MQ=255) gaagaaTTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAg < 5:214687/147‑1 (MQ=255) agaaTTACGCTCCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAACGCAGGCAGACGGAAAAGCCAGTTGC‑TTTGTTCAACGTGGAAAAAGGCCGTATCACCAAGACACACGACCATCGGGAGTGAACGCATGAGCGa > 5:86912/1‑148 (MQ=255) aTTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGTCTTGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAAt > 2:294176/1‑149 (MQ=255) ttACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGTGAAAGAACACACAACAAAACGCCAGTTGC‑TTTGTTCCACGt > 6:273471/1‑89 (MQ=255) cacAGGTGATGAACACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTa > 7:16230/3‑149 (MQ=255) cacAGGTGATGAACACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTa > 3:196326/3‑149 (MQ=255) ccAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTa < 1:294176/148‑1 (MQ=255) ccAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTAc > 1:147227/1‑149 (MQ=255) aGGTGTTCATCACCTGTGTAGATCACGAATTGACTGAGGGAAGGCTGCCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGAATGAGCGAAAATCAAACGTAc > 1:33885/1‑148 (MQ=255) gtgtTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAg < 6:62943/117‑1 (MQ=255) gtgtTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAg > 5:62943/1‑117 (MQ=255) gATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCa > 5:248450/1‑137 (MQ=255) gATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCa < 6:248450/137‑1 (MQ=255) gAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAg < 1:260796/105‑1 (MQ=255) gAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAg > 2:260796/1‑105 (MQ=255) aaTT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTAc > 6:316513/1‑120 (MQ=255) aaTT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTAc < 5:316513/120‑1 (MQ=255) aTT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAAGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGt > 4:26007/1‑149 (MQ=255) t‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGCCCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGttt > 1:136586/1‑149 (MQ=255) cTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCaa < 5:285348/109‑1 (MQ=255) cTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCaa > 6:285348/1‑109 (MQ=255) cTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTg < 8:16230/148‑1 (MQ=255) aGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTa > 2:89251/1‑149 (MQ=255) gCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCg < 5:83199/145‑1 (MQ=255) gCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCg > 6:83199/1‑145 (MQ=255) cAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGc < 8:74356/149‑1 (MQ=255) aGACTGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCAGTATCACCCAGACCCACGACCATCGGGAGTGAAGACATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGCg > 5:209629/1‑149 (MQ=255) aGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCGTCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGCg > 8:79476/1‑149 (MQ=255) gAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGCGTCCCg < 2:33885/149‑1 (MQ=255) aaaCGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGCGTCCCgg > 4:120472/1‑149 (MQ=255) aCGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGCGTCCCGGCa > 5:306915/1‑149 (MQ=255) gCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCGTCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGCGTCCCGGCATg < 7:79476/149‑1 (MQ=255) gCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATg < 7:302080/126‑1 (MQ=255) gCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGc > 3:172308/1‑138 (MQ=255) gCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGc < 4:172308/138‑1 (MQ=255) gCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGAATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATg > 8:302080/1‑126 (MQ=255) ggccttGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGCGTCCCGGCATGTACAtt > 3:78582/5‑149 (MQ=255) c‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGCGTCCCGGCATGt < 3:46980/142‑1 (MQ=255) c‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGCGTCCCGGCATGt > 4:46980/1‑142 (MQ=255) tttGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGCGTCCCGGCATGt < 2:4594/141‑1 (MQ=255) tttGTTCCACGTGGAACAAGGCCGTAGCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGCGTCCCGGCATGt > 1:4594/1‑141 (MQ=255) ttGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGCGTCCCGGCATGTACATTGGc > 5:101819/1‑148 (MQ=255) tGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAAGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGCGTCCCGGCATGTACATTGGCg < 3:26007/148‑1 (MQ=255) cACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGCGTCCCGGCATGTACATTGGCGACAcc > 1:223535/1‑148 (MQ=255) | ATGACTTGCCGTCCGAACTGGATGACCAGCATCGCCGCGCACTGTGCCGCTTGCTTGAAGAATTACGCTGCCAGGTGTTCATCACCTGTGTAGATCACGAATT‑ACTGAGGGAAGGCTGGCAGACGGAAACGCCAGTTGC‑TTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGAAGGCATGAGCGAAAATCAAACGTACGACTCCTCCAGCATCAAGGTGCTGAAAGGTTTGGATGCCGTACGCAAGCGTCCCGGCATGTACATTGGCGACACC > NC_002947/13132‑13425 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |