Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,416,099 C→A 7.5% R157S (CGT→AGT) ‡ PP_1238 → lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,416,0990CA 7.5% 214.3 / 6.6 67R157S (CGT→AGT) ‡PP_1238lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (32/30);  new base A (2/3);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01

GCCCAGCATTCGCCAGCCGAAGTGTGGCCGGTTGCCCGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTCCCATC  >  NC_002947/1415952‑1416244
                                                                                                                                                   |                                                                                                                                                 
gCCCAGCATTCGCCAGCCGAAGTGTGGCCGGTTGCCCGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGc                                                                                                                                                   <  6:182438/148‑1 (MQ=255)
           gCCAGCCGAAGTGTGGCCGGTTGCCCGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCACGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGACGCTGGCCaag                                                                                                                                        <  5:5220/148‑3 (MQ=255)
                 cGAAGTGTGGCCGGTTGCCCGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGc                                                                                                                                   >  1:159214/1‑147 (MQ=255)
                 cGAAGTGTGGCCGGTTGCCCGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGc                                                                                                                                   >  4:108039/1‑147 (MQ=255)
                  gAAGTGTGGCCGGTTGCCCGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCcag                                                                                                                                <  2:253528/149‑1 (MQ=255)
                          gCCGGTTGCCCGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAgcggc                                                                                                                         >  5:66902/1‑148 (MQ=255)
                                   ccGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAgcggc                                                                                                                         <  6:218161/139‑1 (MQ=255)
                                   ccGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAgcggc                                                                                                                         >  5:218161/1‑139 (MQ=255)
                                   ccGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAgg                                                                                                               >  8:12059/1‑149 (MQ=255)
                                   ccGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAgg                                                                                                               >  6:233346/1‑149 (MQ=255)
                                     ggCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTg                                                                                                             >  5:269702/1‑149 (MQ=255)
                                      gCAGTGCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTgc                                                                                                            <  8:192309/149‑1 (MQ=255)
                                          ttcttcGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGt                                                                                                        <  5:233346/149‑1 (MQ=255)
                                           tcttcGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGACGCTGGCCaa                                                                                                                                         >  7:133958/1‑114 (MQ=255)
                                           tcttcGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGACGCTGGCCaa                                                                                                                                         <  8:133958/115‑2 (MQ=255)
                                             ttcGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGc                                                                                                                   <  7:51006/135‑1 (MQ=255)
                                             ttcGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGc                                                                                                                   >  8:51006/1‑135 (MQ=255)
                                               cGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTAc                                                                                                      <  5:101062/146‑1 (MQ=255)
                                                gAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACgtgt                                                                                                  <  5:325460/149‑1 (MQ=255)
                                                gAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACgtg                                                                                                   <  3:110294/148‑1 (MQ=255)
                                                  ggACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTg                                                                                                                                              >  6:230921/1‑103 (MQ=255)
                                                  ggACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTg                                                                                                                                              <  5:230921/103‑1 (MQ=255)
                                                      aaCGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCAt                                                                                            >  7:300213/1‑149 (MQ=255)
                                                       aCGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAgcggc                                                                                                                         >  6:280312/1‑119 (MQ=255)
                                                       aCGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATg                                                                                           <  7:12059/149‑1 (MQ=255)
                                                        cGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCACACGTACGCGCacct                                                                                          <  3:108039/149‑4 (MQ=255)
                                                         ggCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGa                                                                                         <  6:258994/149‑1 (MQ=255)
                                                            ttCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGcc                                                                                      >  8:18211/1‑149 (MQ=255)
                                                               cGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTg                                                                                    <  2:159214/148‑1 (MQ=255)
                                                                gCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGACGCTGGCCaa                                                                                                                                         >  3:66253/1‑93 (MQ=255)
                                                                gCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGACGCTGGCCaa                                                                                                                                         <  4:66253/94‑2 (MQ=255)
                                                                  aTTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATCCCGCCTGGCAGCGGTTCGATGAACTGTCGGCCTCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGc                                                                                  <  6:303370/147‑1 (MQ=255)
                                                                   ttGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTg                                                                               <  6:66902/149‑1 (MQ=255)
                                                                      cTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAg                                                                            >  6:190180/1‑149 (MQ=255)
                                                                        gaggagCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAgcg                                                                          >  4:263374/1‑149 (MQ=255)
                                                                         aggagCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAgcg                                                                          <  1:244473/148‑1 (MQ=255)
                                                                           gagCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAgc                                                                           >  6:140752/1‑145 (MQ=255)
                                                                           gagCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAgc                                                                           <  5:140752/145‑1 (MQ=255)
                                                                                            tGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTAtg                                                      >  8:185250/1‑149 (MQ=255)
                                                                                              aGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTAtgtg                                                    >  5:268975/1‑149 (MQ=255)
                                                                                                 tCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGt                                                  >  8:33249/1‑148 (MQ=255)
                                                                                                  cAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGTACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGt                                                  >  7:249605/1‑147 (MQ=255)
                                                                                                  cAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGt                                                  >  1:248142/1‑147 (MQ=255)
                                                                                                   aaTACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCa                                                <  6:268975/148‑1 (MQ=255)
                                                                                                        cGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTc                                           >  3:76915/1‑148 (MQ=255)
                                                                                                        cGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTc                                           >  3:264651/1‑148 (MQ=255)
                                                                                                         gCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGa                                         <  7:18211/149‑1 (MQ=255)
                                                                                                          ccTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAg                                        <  7:33249/149‑1 (MQ=255)
                                                                                                                    tttCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCcggcgg                              <  2:248142/149‑1 (MQ=255)
                                                                                                                    tttCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCcggcgg                              <  5:190180/149‑1 (MQ=255)
                                                                                                                      tCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCggg                             <  2:93752/148‑1 (MQ=255)
                                                                                                                        gATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGgcag                          <  1:239818/149‑1 (MQ=255)
                                                                                                                         aTGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGgcag                          >  8:140510/1‑148 (MQ=255)
                                                                                                                         aTGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGgcag                          >  1:55938/1‑148 (MQ=255)
                                                                                                                          tGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGgcggca                        <  3:263374/149‑1 (MQ=255)
                                                                                                                                 tCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGcc                   >  2:26302/1‑147 (MQ=255)
                                                                                                                                   ggCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGc               >  4:95825/1‑149 (MQ=255)
                                                                                                                                    gCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGt                                                  >  6:3514/1‑113 (MQ=255)
                                                                                                                                    gCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGt                                                  <  5:3514/113‑1 (MQ=255)
                                                                                                                                      aTCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTg            <  4:264651/149‑1 (MQ=255)
                                                                                                                                          cTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGtt        >  2:262195/1‑149 (MQ=255)
                                                                                                                                           ttGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTc       >  8:265713/1‑149 (MQ=255)
                                                                                                                                               ccAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTCCCAt   >  2:149538/1‑149 (MQ=255)
                                                                                                                                               ccAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTCCCAt   >  8:228498/1‑149 (MQ=255)
                                                                                                                                                cAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTc       <  8:157597/144‑1 (MQ=255)
                                                                                                                                                cAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTc       >  7:157597/1‑144 (MQ=255)
                                                                                                                                                cAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTCCCATc  <  2:99542/149‑1 (MQ=255)
                                                                                                                                                cAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTCCCATc  <  7:228498/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCCCAGCATTCGCCAGCCGAAGTGTGGCCGGTTGCCCGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTCCCATC  >  NC_002947/1415952‑1416244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑