Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,416,101	T→G	7.1%	R157R (CGT→CGG) ‡	PP_1238 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,416,101	0	T	G	7.1%	211.2 / 7.1	71	R157R (CGT→CGG) ‡	PP_1238	lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (34/32);  new base G (2/3);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

GCCAGCCGAAGTGTGGCCGGTTGCCCGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTCCCATCCAC  >  NC_002947/1415963‑1416247                                                                                                                                           |                                                                                                                                                   gCCAGCCGAAGTGTGGCCGGTTGCCCGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCACGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGACGCTGGCCaag                                                                                                                                           <  5:5220/148‑3 (MQ=255)       cGAAGTGTGGCCGGTTGCCCGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGc                                                                                                                                      >  1:159214/1‑147 (MQ=255)       cGAAGTGTGGCCGGTTGCCCGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGc                                                                                                                                      >  4:108039/1‑147 (MQ=255)        gAAGTGTGGCCGGTTGCCCGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCcag                                                                                                                                   <  2:253528/149‑1 (MQ=255)                gCCGGTTGCCCGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAgcggc                                                                                                                            >  5:66902/1‑148 (MQ=255)                         ccGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAgcggc                                                                                                                            >  5:218161/1‑139 (MQ=255)                         ccGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAgcggc                                                                                                                            <  6:218161/139‑1 (MQ=255)                         ccGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAgg                                                                                                                  >  8:12059/1‑149 (MQ=255)                         ccGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAgg                                                                                                                  >  6:233346/1‑149 (MQ=255)                           ggCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTg                                                                                                                >  5:269702/1‑149 (MQ=255)                            gCAGTGCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTgc                                                                                                               <  8:192309/149‑1 (MQ=255)                                ttcttcGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGt                                                                                                           <  5:233346/149‑1 (MQ=255)                                 tcttcGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGACGCTGGCCaa                                                                                                                                            >  7:133958/1‑114 (MQ=255)                                 tcttcGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGACGCTGGCCaa                                                                                                                                            <  8:133958/115‑2 (MQ=255)                                   ttcGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGc                                                                                                                      >  8:51006/1‑135 (MQ=255)                                   ttcGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGc                                                                                                                      <  7:51006/135‑1 (MQ=255)                                     cGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTAc                                                                                                         <  5:101062/146‑1 (MQ=255)                                      gAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACgtgt                                                                                                     <  5:325460/149‑1 (MQ=255)                                      gAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACgtg                                                                                                      <  3:110294/148‑1 (MQ=255)                                        ggACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTg                                                                                                                                                 >  6:230921/1‑103 (MQ=255)                                        ggACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTg                                                                                                                                                 <  5:230921/103‑1 (MQ=255)                                            aaCGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCAt                                                                                               >  7:300213/1‑149 (MQ=255)                                             aCGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAgcggc                                                                                                                            >  6:280312/1‑119 (MQ=255)                                             aCGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATg                                                                                              <  7:12059/149‑1 (MQ=255)                                              cGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCACACGTACGCGCacct                                                                                             <  3:108039/149‑4 (MQ=255)                                               ggCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGa                                                                                            <  6:258994/149‑1 (MQ=255)                                                  ttCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGcc                                                                                         >  8:18211/1‑149 (MQ=255)                                                     cGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTg                                                                                       <  2:159214/148‑1 (MQ=255)                                                      gCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGACGCTGGCCaa                                                                                                                                            >  3:66253/1‑93 (MQ=255)                                                      gCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGACGCTGGCCaa                                                                                                                                            <  4:66253/94‑2 (MQ=255)                                                        aTTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATCCCGCCTGGCAGCGGTTCGATGAACTGTCGGCCTCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGc                                                                                     <  6:303370/147‑1 (MQ=255)                                                         ttGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTg                                                                                  <  6:66902/149‑1 (MQ=255)                                                            cTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAg                                                                               >  6:190180/1‑149 (MQ=255)                                                              gaggagCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAgcg                                                                             >  4:263374/1‑149 (MQ=255)                                                               aggagCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAgcg                                                                             <  1:244473/148‑1 (MQ=255)                                                                 gagCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAgc                                                                              <  5:140752/145‑1 (MQ=255)                                                                 gagCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAgc                                                                              >  6:140752/1‑145 (MQ=255)                                                                                  tGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTAtg                                                         >  8:185250/1‑149 (MQ=255)                                                                                    aGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTAtgtg                                                       >  5:268975/1‑149 (MQ=255)                                                                                       tCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGt                                                     >  8:33249/1‑148 (MQ=255)                                                                                        cAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGTACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGt                                                     >  7:249605/1‑147 (MQ=255)                                                                                        cAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGt                                                     >  1:248142/1‑147 (MQ=255)                                                                                         aaTACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCa                                                   <  6:268975/148‑1 (MQ=255)                                                                                              cGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTc                                              >  3:76915/1‑148 (MQ=255)                                                                                              cGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTc                                              >  3:264651/1‑148 (MQ=255)                                                                                               gCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGa                                            <  7:18211/149‑1 (MQ=255)                                                                                                ccTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAg                                           <  7:33249/149‑1 (MQ=255)                                                                                                          tttCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCcggcgg                                 <  2:248142/149‑1 (MQ=255)                                                                                                          tttCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCcggcgg                                 <  5:190180/149‑1 (MQ=255)                                                                                                            tCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCggg                                <  2:93752/148‑1 (MQ=255)                                                                                                              gATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGgcag                             <  1:239818/149‑1 (MQ=255)                                                                                                               aTGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGgcag                             >  1:55938/1‑148 (MQ=255)                                                                                                               aTGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGgcag                             >  8:140510/1‑148 (MQ=255)                                                                                                                tGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGgcggca                           <  3:263374/149‑1 (MQ=255)                                                                                                                       tCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGcc                      >  2:26302/1‑147 (MQ=255)                                                                                                                         ggCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGc                  >  4:95825/1‑149 (MQ=255)                                                                                                                          gCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGt                                                     <  5:3514/113‑1 (MQ=255)                                                                                                                          gCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGt                                                     >  6:3514/1‑113 (MQ=255)                                                                                                                            aTCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTg               <  4:264651/149‑1 (MQ=255)                                                                                                                                cTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGtt           >  2:262195/1‑149 (MQ=255)                                                                                                                                 ttGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTc          >  8:265713/1‑149 (MQ=255)                                                                                                                                     ccAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTCCCAt      >  2:149538/1‑149 (MQ=255)                                                                                                                                     ccAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTCCCAt      >  8:228498/1‑149 (MQ=255)                                                                                                                                      cAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTc          >  7:157597/1‑144 (MQ=255)                                                                                                                                      cAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTc          <  8:157597/144‑1 (MQ=255)                                                                                                                                      cAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTCCCATc     <  7:228498/149‑1 (MQ=255)                                                                                                                                      cAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTCCCATc     <  2:99542/149‑1 (MQ=255)                                                                                                                                          gTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGaa                                                                <  5:303706/86‑1 (MQ=255)                                                                                                                                          gTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGaa                                                                >  6:303706/1‑86 (MQ=255)                                                                                                                                          gTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTCCCATCCAc  >  5:246640/1‑148 (MQ=255)                                                                                                                                          gTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTCCCATCCAc  <  6:246640/148‑1 (MQ=255)                                                                                                                                           |                                                                                                                                                   GCCAGCCGAAGTGTGGCCGGTTGCCCGGCAGTTCTTCGAGGACAACGGCTTCCGCATTGCTGAGGAGCGCCCGCAGACGGGTGAGTTCAATACCGCCTGGCAGCGTTTCGATGAACTGTCGGCATCGCTTGGCCAGCGTCTGGCCAGCACTGCCAGCAGCGGCGACAGCGAGGTGCGCGTACGTGTGCGCATGGAGCCTGGCGTGCAGCGCAACACCTCCGAAGTGTATGTGGTCAGCGTCGAGCGCCCGGCGGGCAGCACTGCCGAGCCTGCGTTCCCATCCAC  >  NC_002947/1415963‑1416247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑