| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,418,250 | T→A | 9.8% | *237K (TAA→AAA) ‡ | purC → | phosphoribosylaminoimidazole‑succinocarboxamide synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,418,250 | 0 | T | A | 9.8% | 162.0 / 5.1 | 59 | *237K (TAA→AAA) ‡ | purC | phosphoribosylaminoimidazole‑succinocarboxamide synthase |
| Reads supporting (aligned to +/- strand): ref base T (29/24); new base A (3/3); total (32/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGCTGGGCGACGAGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGTTGTACTGGCAA > NC_002947/1418103‑1418398 | gTGCTGGGCGACGAGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGt > 5:59641/1‑148 (MQ=255) cgacgaGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGc < 1:35408/144‑1 (MQ=255) cgacgaGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGc > 2:35408/1‑144 (MQ=255) cgacgaGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCAc < 8:292984/149‑1 (MQ=255) gTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCAc > 8:247771/1‑149 (MQ=255) gTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCAc > 2:231383/1‑149 (MQ=255) ttCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGTCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACg > 3:279432/1‑149 (MQ=255) aGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGc > 5:234815/1‑147 (MQ=255) gCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAg > 1:3813/1‑149 (MQ=255) cGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAgcgc > 3:97273/1‑149 (MQ=255) cTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATAc < 2:3813/149‑1 (MQ=255) gccgccTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCa > 7:167373/1‑149 (MQ=255) gccgccTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCa > 4:152808/1‑149 (MQ=255) ccgccTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTTACAGCGGCACgc < 3:313181/127‑2 (MQ=255) ccgccTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATAcc < 6:24325/147‑1 (MQ=255) cTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGa < 6:234815/149‑1 (MQ=255) ggACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTaa < 6:330776/109‑1 (MQ=255) ggACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTaa > 5:330776/1‑109 (MQ=255) aCAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCAGTAAACGGGCCGGTACCGCAAGCGCCAGCTAACACCCGAatttttt > 7:114017/1‑149 (MQ=255) cAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGACAAAAAGAttt < 4:147812/149‑1 (MQ=255) cAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGAttt < 8:167373/149‑1 (MQ=255) aagaagATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGACGTGCCGCTTACAGCGGCACgcccag > 7:58084/1‑101 (MQ=38) aagaagATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGACGTGCCGCTTACAGCGGCACgcccag < 8:58084/106‑6 (MQ=38) agaagaTGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGc < 4:97273/149‑1 (MQ=255) aCAAGGACCGCTTCCGCCAGGGCCTGGGCGACGGGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTaaaa < 8:75005/149‑1 (MQ=255) cAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTaa > 1:73557/1‑146 (MQ=255) aaGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGTACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTTCGTTTTAAAAg > 4:243541/1‑148 (MQ=255) aaGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAg > 2:13546/1‑148 (MQ=255) ggACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAAc > 6:127991/1‑149 (MQ=255) cgcacgggCCTGGGCGACGTTATCGAAGCCTACGAAGAATTTGCCAAGCGCCGGGGCGTGCCGCTTTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTatg < 8:114017/143‑1 (MQ=255) ggCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGcgcg < 3:152808/149‑1 (MQ=255) gCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCgg > 4:280530/1‑149 (MQ=255) gCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGAATTTTTTGAAAAAAAGATTTTCGTTTTAAAAGAACGCTGGTATGATGCGCgg > 7:276850/1‑149 (MQ=255) ggCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTg < 3:280530/149‑1 (MQ=255) cGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGga < 1:231383/149‑1 (MQ=255) cGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGgagag < 7:247771/149‑1 (MQ=255) cGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGga > 3:86357/1‑146 (MQ=255) aTCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGc > 1:269996/1‑149 (MQ=255) aGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAg > 4:85425/1‑149 (MQ=255) ccTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGTACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTg < 3:243541/149‑1 (MQ=255) ccTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTg < 2:269996/149‑1 (MQ=255) tACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTTAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGt > 7:33994/1‑149 (MQ=255) tACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTatgat > 3:220577/1‑120 (MQ=255) tACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTatgat < 4:220577/120‑1 (MQ=255) cGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGt < 1:13546/147‑1 (MQ=255) gaagaaGTTGCCAAGCGCCTGGGCGTGCCGCTGTAATCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGgaga < 5:254396/132‑1 (MQ=255) gaagaaGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGgaga > 6:254396/1‑132 (MQ=255) gTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGa < 6:59641/149‑1 (MQ=255) cAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGga < 6:251525/119‑1 (MQ=255) cAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGga > 5:251525/1‑119 (MQ=255) cAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGAt > 5:270436/1‑149 (MQ=255) aaGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACgg < 6:249916/141‑1 (MQ=255) aaGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACgg > 5:249916/1‑141 (MQ=255) aaGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGAtt > 3:231415/1‑149 (MQ=255) ccTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGaaa > 8:171549/1‑149 (MQ=255) gggCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATcc > 2:261902/1‑149 (MQ=255) ggCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCg < 2:73557/149‑1 (MQ=255) acGTGCCGCTTACAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGAt < 2:302137/137‑1 (MQ=255) acGTGCCGCTTACAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGAt > 1:302137/2‑138 (MQ=255) gTGCCGCTTACAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGTTg > 8:119748/1‑149 (MQ=255) tAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTATTGAAAAAAAGATTTGCGATTTAAAAGAACGCTGGTTTGATCCGCGCCATTGTAACGTTCCGGCATTGTTGGAACGGTACCTATACGAAATCCTTTGTaataaatc > 5:201678/1‑142 (MQ=255) | GTGCTGGGCGACGAGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGTTGTACTGGCAA > NC_002947/1418103‑1418398 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |