Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,453,977	A→C	14.6%	D204A (GAT→GCT)	PP_1272 →	multidrug MFS transporter membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,453,977	0	A	C	14.6%	104.2 / 11.1	41	D204A (GAT→GCT)	PP_1272	multidrug MFS transporter membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base A (17/18);  new base C (3/3);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GAGCAGGGCGCGGGTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCGGGCGCTCGC  >  NC_002947/1453832‑1454116                                                                                                                                                  |                                                                                                                                             gAGCAGGGCGCGGGTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCATCGGCGCTgg                                                                                                                                           >  8:187799/1‑149 (MQ=255)   gCAGGGCGCGGGTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGcc                                                                                                                                         <  1:137105/149‑1 (MQ=255)       ggCGCGGGTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCATCGGCGCTGGCCactt                                                                                                                                     <  7:187799/149‑4 (MQ=255)          gcgGGTACCGTGCAGAACACCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCATCGGCGCTGGCCacttgag                                                                                                                                  <  4:224034/149‑7 (MQ=255)                  cGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCATCGGCGCTGGCCact                                                                                                                                      >  5:271300/1‑135 (MQ=255)                  cGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCATCGGCGCTGGCCact                                                                                                                                      <  6:271300/137‑3 (MQ=255)                     gCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAg                                                                                                                       >  1:191127/1‑149 (MQ=255)                                      cgcgcAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGcc                                                                                                      <  4:183389/149‑1 (MQ=255)                                             cgcTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTc                                                                                                >  2:20405/1‑148 (MQ=255)                                                      tCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGAt                                                                                             <  3:108873/142‑1 (MQ=255)                                                      tCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGAt                                                                                             >  4:108873/1‑142 (MQ=255)                                                         aGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGAt                                                                                                                                             >  7:285330/1‑91 (MQ=255)                                                         aGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGAt                                                                                                                                             <  8:285330/91‑1 (MQ=255)                                                          gCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGt                                                                                         >  4:222345/1‑142 (MQ=255)                                                          gCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGt                                                                                         <  3:222345/142‑1 (MQ=255)                                                            ccGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACAccc                                                                               <  8:136806/149‑1 (MQ=255)                                                                  tCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGc                                                                             <  1:312918/145‑1 (MQ=255)                                                                  tCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGc                                                                             >  2:312918/1‑145 (MQ=255)                                                                           cAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGTCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACAcc                                                                                <  2:259091/133‑1 (MQ=255)                                                                           cAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGTCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACAcc                                                                                >  1:259091/1‑133 (MQ=255)                                                                            aaCGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCa                                                                                                    <  6:188109/113‑1 (MQ=255)                                                                            aaCGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCa                                                                                                    >  5:188109/1‑113 (MQ=255)                                                                                         cgcgGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATCACAGCGCCGGTGGACGGTATgg                                                    <  1:20405/147‑1 (MQ=255)                                                                                         cgcgGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATCACAGCGCCGGTGGACGGTATGGt                                                   <  1:152943/148‑1 (MQ=255)                                                                                          gcgGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGG‑AAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATCACAGCGCCGGTGGACGGTATGGTCgg                                                <  1:289768/149‑1 (MQ=255)                                                                                                   ggcgCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAg                                                                                                   >  5:198327/1‑91 (MQ=255)                                                                                                   ggcgCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAg                                                                                                   <  6:198327/91‑1 (MQ=255)                                                                                                    gcgcgcAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACgcgc                                       >  4:16546/1‑149 (MQ=255)                                                                                                    gcgcgcAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACgcgc                                       >  2:49435/1‑149 (MQ=255)                                                                                                      gcgcAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGccc                                     >  8:268006/1‑149 (MQ=255)                                                                                                                gTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTggg                           <  8:77898/149‑1 (MQ=255)                                                                                                                   gACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATca                                                                         >  7:228806/1‑100 (MQ=255)                                                                                                                   gACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATca                                                                         <  8:228806/100‑1 (MQ=255)                                                                                                                         cTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGt                  <  7:268006/149‑1 (MQ=255)                                                                                                                           cACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTc                 <  1:8153/148‑1 (MQ=255)                                                                                                                           cACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTc                 >  2:8153/1‑148 (MQ=255)                                                                                                                            aCGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCaa               >  7:203683/1‑149 (MQ=255)                                                                                                                                   aaGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCgg          <  8:203683/147‑1 (MQ=255)                                                                                                                                   aaGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCGGgc        >  8:277726/1‑149 (MQ=255)                                                                                                                                     ctGGCCATCGGCGCTGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCGGgcgc      >  5:130479/2‑149 (MQ=255)                                                                                                                                          cAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCTGACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCGGGCGCTCGc  >  1:257658/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                             GAGCAGGGCGCGGGTACCGTGCAGAACGCCCAGCAGGCGCGCAGCGCTGTCGATCAAGCCCGTGCTCGCCTGGCCAACGCTCAAGCTGCGCGGGTGGCGGCGCGCAAGCAGGTGGACATCCTCACGGCTCAAGTGGCCAGCGCCGATGGCCAGCTCAAGCGTGCCGAAGCAGGCCTGGAAAAGGCCCAGCTCGATCTGTCCT‑ACACCCGCATCACAGCGCCGGTGGACGGTATGGTCGGCGAACGCGCCCTGCGCGTGGGGGCCTACGTCAACCCGGGCGCTCGC  >  NC_002947/1453832‑1454116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑