Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,458,121 C→A 8.0% intergenic (+21/+19) PP_1276 → / ← algA hypothetical protein/mannose‑6‑phosphate isomerase/mannose‑1‑phosphate guanylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,458,1210CA 8.0% 150.1 / 3.7 50intergenic (+21/+19)PP_1276/algAhypothetical protein/mannose‑6‑phosphate isomerase/mannose‑1‑phosphate guanylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (21/25);  new base A (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

TCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTCG  >  NC_002947/1457973‑1458257
                                                                                                                                                    |                                                                                                                                        
tCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGc                                                                                                                                          >  7:196675/1‑149 (MQ=255)
        aCTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAgggg                                                                                                                                   <  8:196675/148‑1 (MQ=255)
            ccACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGt                                                                                                                              >  4:146619/1‑149 (MQ=255)
              acaAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCa                                                                                                                            <  2:130370/149‑1 (MQ=255)
                aaGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCa                                                                                                                            <  1:203109/147‑1 (MQ=255)
                aaGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCa                                                                                                                            >  2:203109/1‑147 (MQ=255)
                   cATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCAtt                                                                                                                       <  5:118261/149‑1 (MQ=255)
                     tCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTa                                                                                                                     <  7:262347/149‑1 (MQ=255)
                     tCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTa                                                                                                                     <  2:209482/149‑1 (MQ=255)
                     tCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTa                                                                                                                     >  1:209482/1‑149 (MQ=255)
                              aaTCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGAt                                                                                                            >  5:213950/1‑149 (MQ=255)
                                  cTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTc                                                                                                        <  2:139289/149‑1 (MQ=255)
                                    tCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCtt                                                                                                      >  3:45281/1‑149 (MQ=255)
                                       aaTTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCAc                                                                                                   <  3:182697/149‑1 (MQ=255)
                                        aTTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCAcc                                                                                                  >  2:256574/1‑149 (MQ=255)
                                         tttGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCg                                                                                                 <  4:45281/149‑1 (MQ=255)
                                         tttGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGAAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCAcg                                                                                                  >  5:188886/1‑147 (MQ=255)
                                            gAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGaaa                                                                                              >  2:158316/1‑149 (MQ=255)
                                               ggggAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCGCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAAcgc                                                                                           <  1:40561/149‑1 (MQ=255)
                                                       tgatTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTc                                                                                    >  4:234307/1‑148 (MQ=255)
                                                              cAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTAc                                                                                                                    >  5:327430/1‑109 (MQ=255)
                                                              cAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTAc                                                                                                                    <  6:327430/109‑1 (MQ=255)
                                                              cAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATgggg                                                                             <  3:270402/148‑1 (MQ=255)
                                                                     cTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGt                                                                     <  6:213950/149‑1 (MQ=255)
                                                                          aGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTgcgc                                                                 <  3:146619/148‑1 (MQ=255)
                                                                          aGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTgcgc                                                                 <  1:256574/148‑1 (MQ=255)
                                                                                gtgggtggCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATaca                                                          >  1:216124/1‑149 (MQ=255)
                                                                                 tgggtggCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATacac                                                         <  2:216124/149‑1 (MQ=255)
                                                                                        cccATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGGCTGACCAggg                                                                                                                                    <  7:24363/67‑1 (MQ=38)
                                                                                        cccATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGGCTGACCAggg                                                                                                                                    >  8:24363/1‑67 (MQ=38)
                                                                                         ccATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCg                                                  >  2:5185/1‑148 (MQ=255)
                                                                                         ccATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCg                                                  >  1:63194/1‑148 (MQ=255)
                                                                                          cATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGaa                                                <  3:234307/149‑1 (MQ=255)
                                                                                          cATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGaa                                                <  1:5185/149‑1 (MQ=255)
                                                                                                        gTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATac                                                           <  5:253285/124‑1 (MQ=255)
                                                                                                        gTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATac                                                           >  6:253285/1‑124 (MQ=255)
                                                                                                        gTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGCAGCGCACGATGTCAt                                  >  5:323184/1‑149 (MQ=255)
                                                                                                             aGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCg                             >  1:121699/1‑149 (MQ=255)
                                                                                                                   gAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAgg                       >  7:304230/1‑149 (MQ=255)
                                                                                                                      cGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAg                    <  8:304230/149‑1 (MQ=255)
                                                                                                                         tGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTc                 >  8:27106/1‑149 (MQ=255)
                                                                                                                                gCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCt                                                    <  4:134773/107‑1 (MQ=255)
                                                                                                                                gCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCt                                                    >  3:134773/1‑107 (MQ=255)
                                                                                                                                gCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACt          >  5:286022/1‑149 (MQ=255)
                                                                                                                                  aGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGc        <  2:121699/149‑1 (MQ=255)
                                                                                                                                     cTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCAcc     <  6:188886/149‑1 (MQ=255)
                                                                                                                                     cTCGCCCCTGGGCTGACCAGGGGCGAGCCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGaa                                                >  2:97769/1‑106 (MQ=39)
                                                                                                                                     cTCGCCCCTGGGCTGACCAGGGGCGAGCCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGaa                                                <  1:97769/106‑1 (MQ=39)
                                                                                                                                        gCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTCg  <  6:323184/149‑1 (MQ=255)
                                                                                                                                          cccTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGAACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTGATCCTCGCCCAGGTAGCTCCCg              >  5:306921/1‑135 (MQ=255)
                                                                                                                                          cccTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGAACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCg              <  6:306921/135‑1 (MQ=255)
                                                                                                                                                    cccAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGAGCTCGATGTCATCCTCGCCCAGGTAGCTcc                >  2:191844/1‑123 (MQ=255)
                                                                                                                                                    |                                                                                                                                        
TCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTCG  >  NC_002947/1457973‑1458257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑