Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,465,873 T→A 11.6% K440* (AAG→TAG) ‡ algG ← poly(beta‑D‑mannuronate) C5 epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,465,8730TA11.6% 112.5 / 5.5 43K440* (AAG→TAG) ‡algGpoly(beta‑D‑mannuronate) C5 epimerase
Reads supporting (aligned to +/- strand):  ref base T (21/17);  new base A (3/2);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

CCAGCACACCATTGAGGCTGATGCCGCTGGCCTTGCGCGGGGCGAGCATGGACACCTTGTACAGCTCGACCGACAGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCGGATGTTCACGCTGTTACGCACGCGGATGCCGTGGCGGCG  >  NC_002947/1465726‑1466020
                                                                                                                                                   |                                                                                                                                                    
ccAGCACACCATTGAGGCTGATGCCGCTGGCCTTGCGCGGGGCGAGCATGGACACCTTGTACAGCTCGACCGACAGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTg                                                                                                                                                     >  4:20304/1‑149 (MQ=255)
    cacaCCATTGAGGCTGATGCCGCTGGCCTTGCGCGGGGCGAGCATGGACACCTTGTACAGCTCGACCGACAGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCAAGgtgt                                                                                                                                                 >  1:65088/1‑149 (MQ=255)
       acCATTGAGGCTGATGCCGCTGGCCTTGCGCGGGGCGAGCATGGACACCTTGTACAGCTCGACCGACAGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCAAGGTGTCgc                                                                                                                                              <  2:65088/149‑2 (MQ=255)
                gCTGATGCCGCTGGCCTTGCGCGGGGCGAGCATGGACACCTTGTACAGCTCGACCGACAGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑c                                                                                                                                      >  1:103129/1‑147 (MQ=255)
                      gCCGCTGGCCTTGCGCGGGGCGAGCATGGACACCTTGTACAGCTCGACCGACAGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCg                                                                                                                               <  8:172973/148‑1 (MQ=255)
                       ccGCTGGCCTTGCGCGGGGCGAGCATGGACACCTTGTACAGCTCGACCGACAGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGCCACCAAGGTGTCgctg                                                                                                                                            >  5:213176/1‑132 (MQ=255)
                        cGCTGGCCTTGCGCGGGGCGAGCATGGACACGTTGTACAGCTCGACCGACTGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATg                                                                                                                            <  3:50379/149‑1 (MQ=255)
                                            aGCATGGACACCTTGTACAGCTCGACCGACAGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGGGGTCGGTGTCGGACAg                                                                                                        >  7:236531/1‑149 (MQ=255)
                                            aGCATGGACACCTTGTACAGCTCGACCGACAGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAg                                                                                                        <  5:73623/149‑1 (MQ=255)
                                              cATGGACACCTTGTACAGCTCGACCGACAGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGt                                                                                                      <  5:168960/149‑1 (MQ=255)
                                                  gACACCTTGTACAGCTCGACCGACAGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCtt                                                                                                   <  7:127830/148‑1 (MQ=255)
                                                  gACACCTTGTACAGCTCGACCGACAGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCAGACAGGTCttt                                                                                                  <  3:20304/149‑1 (MQ=255)
                                                   acacCTTGTACAGCTCGACCGACAGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCttt                                                                                                  >  8:257900/1‑148 (MQ=255)
                                                               gCTCGACCGACAGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGta                                                                                      >  8:245192/1‑148 (MQ=255)
                                                                       gacAGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGAc                                                                             <  7:245192/149‑1 (MQ=255)
                                                                                   tcgatcgaCAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGc                                                                 <  8:79745/149‑1 (MQ=255)
                                                                                      atcgaCAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCAt                                                              >  6:242388/1‑149 (MQ=255)
                                                                                          aCAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGc                                                           >  3:108355/1‑148 (MQ=255)
                                                                                          aCAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGc                                                           >  6:231513/1‑148 (MQ=255)
                                                                                           cAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGa                                                         >  5:78540/1‑149 (MQ=255)
                                                                                                  ccGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTc                                                  <  7:257900/149‑1 (MQ=255)
                                                                                                  ccGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTc                                                  <  5:242388/149‑1 (MQ=255)
                                                                                                    ggAGCCGTTGGACGACAGCTCGCCACACACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCg                                                 >  3:306008/1‑148 (MQ=255)
                                                                                                      aGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTTGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTa                                               >  4:283929/1‑148 (MQ=255)
                                                                                                           ttGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCg                                         >  8:196538/1‑149 (MQ=255)
                                                                                                             ggCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGAc                                                                             <  2:220362/111‑1 (MQ=255)
                                                                                                              gCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCGGAt                                      >  5:96681/1‑149 (MQ=255)
                                                                                                              gCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCGGAt                                      <  5:231513/149‑1 (MQ=255)
                                                                                                               ccGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCGGATg                                     >  6:185553/1‑149 (MQ=255)
                                                                                                                cGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCGGATGt                                    <  4:304300/149‑1 (MQ=255)
                                                                                                                      cTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTc                                                  >  5:248473/1‑129 (MQ=255)
                                                                                                                      cTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTc                                                  <  6:248473/129‑1 (MQ=255)
                                                                                                                         gCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCGGATGTTCACGCTGt                           >  5:284118/1‑149 (MQ=255)
                                                                                                                         gCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCGGATGTTCACGCTGt                           >  5:289193/1‑149 (MQ=255)
                                                                                                                            acccaccACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCGGATGTTCACGCTGTTac                        <  8:236531/149‑1 (MQ=255)
                                                                                                                              ccaccaCGATCAGCGACACCTTGGTGTCGAACGGGTGCGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCGGATGTTCACGCTGTTacg                       >  1:215567/1‑149 (MQ=255)
                                                                                                                               caccacGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCGGATGTTCACGCTGTTacgca                     >  8:148759/1‑149 (MQ=255)
                                                                                                                                  cacGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCGGATGTTCACGCTGTTacgcacgc                  >  4:251982/1‑149 (MQ=255)
                                                                                                                                       cgttcGACACCAAGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCGGATGTTCACGCTGTTACGCACGCGGATg             >  2:107136/5‑149 (MQ=255)
                                                                                                                                          gCGACACCTTGGTGTCGAACGGGT‑CGAGGGTGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCGGATGTTCACGCTGTTACGCACGCGGATGCCg          >  2:227174/1‑149 (MQ=255)
                                                                                                                                          gCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCGGATGTTCACGCTGTTACGCACGCGGATGCCg          <  4:108355/149‑1 (MQ=255)
                                                                                                                                            gACACGTTGGTGTCGAACTGGT‑CGAGGGCTATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCGGTTGGCCATTGCGACGTTTTCGTACAGGCGGATGTTCACGCTGTTAGGCACGCGGATGCCGTg        <  4:306008/149‑1 (MQ=255)
                                                                                                                                            gACACCAAGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCGGATGTTCACGCTGTTACGCACGCGGATGCCGTg        <  1:107136/149‑1 (MQ=255)
                                                                                                                                                  ttGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCTTGGCGACGTTTTCGTACAGGCGGATGTTCACGCTGTTACGCACGCGGATGCCGTGgcggcg  >  3:91428/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCAGCACACCATTGAGGCTGATGCCGCTGGCCTTGCGCGGGGCGAGCATGGACACCTTGTACAGCTCGACCGACAGCGGCGAGTCGATCGACAGCGGGCCGGAGCCGTTGGCCGACAGCTCGCCACCCACCACGATCAGCGACACCTTGGTGTCGAACGGGT‑CGAGGGCGATGTCGCGGTCGGTGTCGGACAGGTCTTTGATGTGGCCGTATACGCCGACCAGTCCGTTGGCCATGGCGACGTTTTCGTACAGGCGGATGTTCACGCTGTTACGCACGCGGATGCCGTGGCGGCG  >  NC_002947/1465726‑1466020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑