Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 182,035 A→T 10.7% intergenic (+24/+41) PP_5SB → / ← PP_0160 5S ribosomal RNA/ferrioxamine receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947182,0350AT10.7% 146.2 / 8.9 56intergenic (+24/+41)PP_5SB/PP_01605S ribosomal RNA/ferrioxamine receptor
Reads supporting (aligned to +/- strand):  ref base A (21/29);  new base T (3/3);  total (24/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

ACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGC  >  NC_002947/181898‑182183
                                                                                                                                         |                                                                                                                                                    
acgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCATCGCAGACAggg                                                                                                                                           <  5:265424/149‑1 (MQ=255)
    ccATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCtt                                                                                                                                       >  2:117405/1‑149 (MQ=255)
     cATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTCAAACTATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAACCCCCTGTCTGCGATGCAGACAGGGGCttt                                                                                                                                      <  1:169371/149‑1 (MQ=255)
      aTAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTg                                                                                                                                     >  6:181312/1‑149 (MQ=255)
       tAGAGCATTCGAACCACCTGATCCCAACCCGAACTCAGCAGTGAAAGGATGCATCGCCGATGGTAGTGTTCGGTTTCCCCATGGGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGTCAGGGGCTTTGt                                                                                                                                    <  5:270434/149‑1 (MQ=17)
         gagCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCt                                                                                                                                  >  3:60184/1‑149 (MQ=255)
             aTTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCATCGCAGACAgg                                                                                                                                            <  5:258563/135‑1 (MQ=255)
             aTTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCATCGCAGACAgg                                                                                                                                            >  6:258563/1‑135 (MQ=255)
               tGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCg                                                                                                                            >  7:79125/1‑149 (MQ=255)
                 gAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCggg                                                                                                                          >  2:8690/1‑149 (MQ=255)
                 gAACCACCTGATCCCATCCCGAACACAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCggg                                                                                                                          <  8:273113/149‑1 (MQ=255)
                        cTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAat                                                                                                                   <  2:63364/149‑1 (MQ=255)
                           atcccatcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATc                                                                                                                <  5:181312/149‑1 (MQ=255)
                                   ccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGaa                                                                                                        <  5:164166/149‑1 (MQ=255)
                                   ccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGaa                                                                                                        <  1:8690/149‑1 (MQ=255)
                                    cGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAAc                                                                                                       >  7:244621/1‑149 (MQ=255)
                                    cGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCATCGCAGACAGGGGCttt                                                                                                                                      <  6:250806/118‑1 (MQ=255)
                                    cGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCATCGCAGACAGGGGCttt                                                                                                                                      >  5:250806/1‑118 (MQ=255)
                                        cTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGAt                                                                                                   <  2:307459/149‑1 (MQ=255)
                                          cagcagTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGAt                                                                                                   >  2:248343/1‑147 (MQ=255)
                                          cagcagTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGAt                                                                                                   <  1:248343/147‑1 (MQ=255)
                                                       aTGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGgcgc                                                                                    >  2:299544/1‑149 (MQ=255)
                                                           aTCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTca                                                                                 >  2:285462/1‑148 (MQ=255)
                                                           aTCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTca                                                                                 >  1:205265/1‑148 (MQ=255)
                                                              gCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACAtt                                                                             >  6:280014/1‑149 (MQ=255)
                                                                 gATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTgcg                                                                          <  5:280014/149‑1 (MQ=255)
                                                                 gATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTgcg                                                                          <  8:97171/149‑1 (MQ=255)
                                                                  aTGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACCGATAGCTGACCGTGGTGCTCACATTgcgc                                                                         <  8:244621/149‑1 (MQ=255)
                                                                             gggTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCa                                                               <  4:60184/148‑1 (MQ=255)
                                                                                    cccATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCag                                                       <  2:144940/149‑1 (MQ=255)
                                                                                    cccATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCag                                                       <  2:205265/149‑1 (MQ=255)
                                                                                      cATGTGAGAGTAGGTCATAGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCagta                                                     <  8:79125/149‑1 (MQ=255)
                                                                                             gattAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAg                                               <  4:294249/145‑1 (MQ=255)
                                                                                             gagTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAgg                                              <  1:117405/149‑1 (MQ=255)
                                                                                             gagTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAgg                                              <  1:52479/149‑1 (MQ=255)
                                                                                                  ggTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTg                                           <  4:92458/147‑1 (MQ=255)
                                                                                                         gTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAg                                  >  8:25806/1‑149 (MQ=255)
                                                                                                          tCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAgg                                 >  3:193884/1‑149 (MQ=255)
                                                                                                            aaGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGt                                >  2:192072/1‑148 (MQ=255)
                                                                                                                ttCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGt                           <  5:194348/149‑1 (MQ=255)
                                                                                                                 tCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATg                                    >  3:231321/1‑139 (MQ=255)
                                                                                                                 tCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATg                                    <  4:231321/139‑1 (MQ=255)
                                                                                                                 tCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATg                            >  5:70360/1‑147 (MQ=255)
                                                                                                                   aaaCCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGc                        >  4:130671/1‑149 (MQ=255)
                                                                                                                         aaaGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCTGGCTGGCACATGAGGTGATGTAGCTTTCAt                  <  1:192072/149‑1 (MQ=255)
                                                                                                                            gCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTTGCACATGAGGTGATGTAGCTTTCATTgg               >  4:76965/1‑149 (MQ=255)
                                                                                                                             ccccTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGt              <  1:299544/149‑1 (MQ=255)
                                                                                                                              cccTGTCTGCATCGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGt              >  3:172758/1‑148 (MQ=255)
                                                                                                                               ccTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAg                                                          >  5:110198/1‑103 (MQ=255)
                                                                                                                               ccTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAg                                                          <  6:110198/103‑1 (MQ=255)
                                                                                                                                cTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAg           <  4:121978/149‑1 (MQ=255)
                                                                                                                                       cGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTCGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGgttgtta    <  6:70360/149‑2 (MQ=255)
                                                                                                                                       cGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCa                   >  3:200162/1‑134 (MQ=255)
                                                                                                                                       cGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCa                   <  4:200162/134‑1 (MQ=255)
                                                                                                                                       cGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGttgttg    >  2:100104/1‑149 (MQ=255)
                                                                                                                                       cGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGttgttg    <  4:193884/149‑1 (MQ=255)
                                                                                                                                         aTGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGc  <  1:285462/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                    
ACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGC  >  NC_002947/181898‑182183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑