Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,495,650 G→A 8.7% A27T (GCC→ACC)  mdeA → methionine gamma‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,495,6500GA 8.7% 158.9 / 4.7 58A27T (GCC→ACC) mdeAmethionine gamma‑lyase
Reads supporting (aligned to +/- strand):  ref base G (27/25);  new base A (2/3);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-01

GCTATTTTGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCATG  >  NC_002947/1495505‑1495793
                                                                                                                                                 |                                                                                                                                               
gCTATTTTGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGccc                                                                                                                                              <  3:58246/149‑1 (MQ=255)
  tATTTTGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTg                                                                                                                                            >  8:22524/1‑149 (MQ=255)
    ttttGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTgg                                                                                                                                           <  7:305455/148‑1 (MQ=255)
    ttttGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGt                                                                                                                                          <  5:29437/149‑1 (MQ=255)
        ggAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGCTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCt                                                                                                                                             >  3:43801/1‑142 (MQ=255)
        ggAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACAACAAGAGGCTGTTGACATGCGCGACTCCCACAACAACAACGGTTTTTCCACGCGGGCCATTCATAATGGCTATGAACAGCTTTCCCATGGTGGCGCCCTgagatcg                                                                                                                                      >  3:47965/1‑143 (MQ=255)
           aaaaaTCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCg                                                                                                                                   <  3:251295/149‑1 (MQ=255)
            aaaaTCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGCCTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCgg                                                                                                                                  >  1:224872/1‑149 (MQ=255)
             aaaTCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCC‑CGGTGGCACCAGGGCGCCAccatgg                                                                                                                                <  1:31060/149‑5 (MQ=255)
                  gggCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTAc                                                                                                                             <  4:92707/148‑1 (MQ=255)
                                cATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTAt                                                                                                              <  7:22524/149‑1 (MQ=255)
                                 ctAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTAt                                                                                                              <  1:115733/147‑1 (MQ=255)
                                  tAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGc                                                                                                            <  3:51752/149‑1 (MQ=255)
                                             cAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTg                                                                                                 >  1:255103/1‑149 (MQ=255)
                                                aCCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCg                                                                                              <  2:84094/149‑1 (MQ=255)
                                                       gagGCTGTTTCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACgg                                                                                       >  7:298095/1‑149 (MQ=255)
                                                             gTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCAc                                                                                                                                     >  5:298311/1‑97 (MQ=255)
                                                             gTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCAc                                                                                                                                     <  6:298311/97‑1 (MQ=255)
                                                                    tGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGATGCCTGCTTc                                                                          >  7:284781/1‑149 (MQ=255)
                                                                       gcgACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGcc                                                                       >  3:144624/1‑149 (MQ=255)
                                                                       gcgACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGcc                                                                       <  2:255103/149‑1 (MQ=255)
                                                                       gcgACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGcc                                                                       >  1:165066/1‑149 (MQ=255)
                                                                        cgACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCg                                                                      >  7:33743/1‑149 (MQ=255)
                                                                            tCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCggg                                                                    >  3:237098/1‑147 (MQ=255)
                                                                            tCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCACCAGGGCGCCa                                                                                                                                      <  6:164094/81‑1 (MQ=255)
                                                                            tCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCACCAGGGCGCCa                                                                                                                                      >  5:164094/1‑81 (MQ=255)
                                                                              ccaccaCAACACCGGTTTTTCCACGCGGGCCAGTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGggag                                                                 >  6:89125/1‑148 (MQ=255)
                                                                                    caacaCCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTg                                                          >  8:97051/1‑149 (MQ=255)
                                                                                    caacaCCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTg                                                          >  5:207722/1‑149 (MQ=255)
                                                                                     aacaCCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTgg                                                         <  2:224872/149‑1 (MQ=255)
                                                                                     aacaCCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTgg                                                         >  8:138118/1‑149 (MQ=255)
                                                                                             tttttCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGggag                                                                 >  8:150556/1‑133 (MQ=255)
                                                                                               tttCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTa                                                <  4:135840/148‑1 (MQ=255)
                                                                                                   cACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGcc                                           >  5:281646/1‑149 (MQ=255)
                                                                                                    aCGCGGGCCATTAATCATGGCTATGACCCGCTTTCCCATGGTGGCTCCCTGGTGCCACAGGTGTACCAGACGGCTACCTAAGCCTTCCCCACTGTCGAGTACGGTGCTGACTGCTTCGCCCGGGAGGAGCCTGGGCATTTCTACAGCCg                                          >  4:194474/1‑149 (MQ=255)
                                                                                                     cgcgGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGc                                         <  6:65384/149‑1 (MQ=255)
                                                                                                           ccATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTcc                                   <  7:138118/149‑1 (MQ=255)
                                                                                                              ttCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTa                                                <  2:239136/133‑1 (MQ=255)
                                                                                                              ttCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTa                                                >  1:239136/1‑133 (MQ=255)
                                                                                                              ttCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCaa                                 <  8:119086/148‑1 (MQ=255)
                                                                                                              ttCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCaa                                 >  7:119086/1‑148 (MQ=255)
                                                                                                                    atGGCTATGACCCGCTTTCCCATGGGGGCGCCCTGGTGCCAGCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACg                          <  8:284781/149‑1 (MQ=255)
                                                                                                                       gCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTg                       <  6:207722/149‑1 (MQ=255)
                                                                                                                        cTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGcc                                           >  2:171177/1‑128 (MQ=255)
                                                                                                                        cTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGcc                                           <  1:171177/128‑1 (MQ=255)
                                                                                                                         tATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCggg                                                                    <  2:191147/102‑1 (MQ=255)
                                                                                                                         tATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCggg                                                                    >  1:191147/1‑102 (MQ=255)
                                                                                                                         tATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAAccc                              <  6:273711/140‑1 (MQ=255)
                                                                                                                         tATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAAccc                              >  5:273711/1‑140 (MQ=255)
                                                                                                                            gACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCtt                  >  7:41985/1‑149 (MQ=255)
                                                                                                                              cccGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCAtt                                                    >  6:203476/1‑113 (MQ=255)
                                                                                                                              cccGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCAtt                                                    <  5:203476/113‑1 (MQ=255)
                                                                                                                                   tttCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTGCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGag           <  3:194474/149‑1 (MQ=255)
                                                                                                                                       ccATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGagcagc       >  7:263679/1‑149 (MQ=255)
                                                                                                                                       ccATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGagcagc       >  8:303504/1‑149 (MQ=255)
                                                                                                                                       ccATGGTGGCGCCCTGGTGCCACCGGTGTACCAGAAGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGGCTGGGCATTTCTACAGCCGCATTTCCAAGCCCACGCTGGCCTTGCTGGagcagc       <  8:298095/149‑1 (MQ=255)
                                                                                                                                            gtggCACCAGGGCGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCATg  >  6:215912/1‑149 (MQ=255)
                                                                                                                                            gtggCACCAGGGCGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCATg  <  5:215912/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GCTATTTTGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCATG  >  NC_002947/1495505‑1495793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑