Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,533,133 C→G 10.0% H382D (CAC→GAC) ‡ secA → protein translocase subunit SecA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,533,1330CG10.0% 148.0 / 7.1 50H382D (CAC→GAC) ‡secAprotein translocase subunit SecA
Reads supporting (aligned to +/- strand):  ref base C (21/24);  new base G (3/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

TTGCTGACACACGTCTACGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCTGTACACCAAGCTGTCCGGCATGACCGGTACCGCCGACACCGAAGCATTCGAGT  >  NC_002947/1532989‑1533274
                                                                                                                                                |                                                                                                                                             
ttGCTGACACACGTCTACGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCAc                                                                                                                                             >  8:123172/1‑147 (MQ=255)
  gCTGACACACGTCTACGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAg                                                                                                                                          <  5:252027/148‑1 (MQ=255)
   cTGACACACGTCTACGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAgg                                                                                                                                         <  1:26514/148‑1 (MQ=255)
      acacacGTCTACGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCAt                                                                                                                                     >  1:267639/1‑149 (MQ=255)
        acacGTCTACGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTTCGATGGCCTGGTGCAGGCCCTCg                                                                                                                                   >  4:162668/1‑149 (MQ=255)
        acacGTCTACGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTTCGATGGCCTGGTGCAGGCCCTCg                                                                                                                                   <  3:162668/149‑1 (MQ=255)
          acGTCTACGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCg                                                                                                                                   >  3:218793/1‑147 (MQ=255)
          acGTCTACGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGaa                                                                                                                                 <  6:68851/149‑1 (MQ=255)
             tCTACGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCAc                                                                                                                                             <  5:207227/134‑1 (MQ=255)
             tCTACGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCAc                                                                                                                                             >  6:207227/1‑134 (MQ=255)
             tCTACGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCAt                                                                                                                                     <  4:106006/142‑1 (MQ=255)
             tCTACGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCAt                                                                                                                                     >  3:106006/1‑142 (MQ=255)
               tACGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAg                                                                                                                                          <  1:120165/135‑1 (MQ=255)
               tACGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAg                                                                                                                                          >  2:120165/1‑135 (MQ=255)
                 cGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCaaa                                                                                                                           <  5:248512/148‑1 (MQ=255)
                     ggTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGaa                                                                                                                      >  8:237046/1‑149 (MQ=255)
                     ggTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGaa                                                                                                                      <  4:218793/149‑1 (MQ=255)
                           cGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGa                                                                                                                 <  6:228744/148‑1 (MQ=255)
                            gTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGaa                                                                                                                >  5:203886/1‑148 (MQ=255)
                                  acaAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGc                                                                                                                                               <  3:51893/111‑1 (MQ=255)
                                  acaAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGc                                                                                                                                               >  4:51893/1‑111 (MQ=255)
                                  acaAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAg                                                                                                         >  1:312404/1‑149 (MQ=255)
                                  acaAGCTCTTCCACCGCCACGTAGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAg                                                                                                         <  6:224971/149‑1 (MQ=255)
                                                         gAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTc                                                                                  <  6:45057/149‑1 (MQ=255)
                                                          aGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGTCGA‑AGCCAGGGTCTGGCtctcg                                                                                >  1:158638/1‑145 (MQ=255)
                                                           gTACATCGTCCAGGACGGCTAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGc                                                                                                                                               <  1:78990/86‑1 (MQ=255)
                                                           gTACATCGTCCAGGACGGCTAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGc                                                                                                                                               >  2:78990/1‑86 (MQ=255)
                                                           gTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGa                                                                                <  4:126290/149‑1 (MQ=255)
                                                              cATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGacca                                                                             >  8:139080/1‑149 (MQ=255)
                                                                tCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGaccacc                                                                           <  2:267639/149‑1 (MQ=255)
                                                                   tCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTc                                                                        <  7:123172/149‑1 (MQ=255)
                                                                      aGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAg                                                                     <  7:139080/149‑1 (MQ=255)
                                                                        gACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGaa                                                                   <  6:203886/149‑1 (MQ=255)
                                                                        gACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGaa                                                                   <  2:312404/149‑1 (MQ=255)
                                                                                    ctgctgATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCt                                                       <  7:237046/149‑1 (MQ=255)
                                                                                       ctgATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCTGTa                                                    >  1:200318/1‑149 (MQ=255)
                                                                                         gATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTgg                                                                                      <  8:151504/113‑1 (MQ=255)
                                                                                         gATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTgg                                                                                      >  7:151504/1‑113 (MQ=255)
                                                                                             gacgaACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCg                                                                                 <  8:61989/114‑1 (MQ=255)
                                                                                             gacgaACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCg                                                                                 >  7:61989/1‑114 (MQ=255)
                                                                                             gacgaACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCTGTACAcc                                                >  8:296656/1‑147 (MQ=255)
                                                                                                gaACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCTGTACACCAAGCt                                           >  7:216265/1‑149 (MQ=255)
                                                                                                       ccGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCTGTACACCAAGCTGTCCGGc                                    <  2:35190/149‑1 (MQ=255)
                                                                                                                 cATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCTGTa                                                    >  3:62754/1‑123 (MQ=255)
                                                                                                                 cATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCTGTa                                                    <  4:62754/123‑1 (MQ=255)
                                                                                                                                ccTGTCCGAGGGCCTGCCCCAGGCAATCGAAGCCACATAGCACCTGAACATCCCGGCCGAGAGCCAGACACTGGCTTCGCCCCCCATCCCGACCTACTTCCGCCTGTACACCCAGCTGGCCGGCATGACCGGTACCGCCTACACCgcat           >  5:177589/1‑146 (MQ=255)
                                                                                                                                  gcttcGATGGCCTGGTGCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCTGTACACCAAGCTGTCCGGCATGACCGGTACCGCCGACACCGAAGCa         >  7:13102/5‑149 (MQ=255)
                                                                                                                                    ttcGATGGCCTGGTGCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCTGTACACCAAGCTGTCCGGCATGACCGGTAccgcc                     >  6:148043/3‑135 (MQ=255)
                                                                                                                                    ttcGATGGCCTGGTGCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCTGTACACCAAGCTGTCCGGCATGACCGGTAccgcc                     <  5:148043/133‑1 (MQ=255)
                                                                                                                                       gAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCTGTACACCAAGCTGTCCGGCATGACCGGTAccgc                      <  7:296031/131‑1 (MQ=255)
                                                                                                                                       gAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCTGTACACCAAGCTGTCCGGCATGACCGGTAccgc                      >  8:296031/1‑131 (MQ=255)
                                                                                                                                         gggCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCTGTACACCAAGCTGTCCGGCATGACCGGTACCGCCGACACCGAAGCATTCGAGt  >  2:68977/1‑149 (MQ=255)
                                                                                                                                         gggCCTGCACCAGGCCATCGAAGCCAAAGAAAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCTGTac                                                   <  7:263424/100‑1 (MQ=255)
                                                                                                                                         gggCCTGCACCAGGCCATCGAAGCCAAAGAAAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCTGTac                                                   >  8:263424/1‑100 (MQ=255)
                                                                                                                                                |                                                                                                                                             
TTGCTGACACACGTCTACGCCGGTCTTCGTGCACACAAGCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGACGGCCAGGTTCTGCTGATCGACGAACACACCGGCCGTACCATGCCGGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAAGCCAAAGAGAACCTGAACATCCAGGCCGAGAGCCAGACCCTGGCTTCGACCACCTTCCAGAACTACTTCCGCCTGTACACCAAGCTGTCCGGCATGACCGGTACCGCCGACACCGAAGCATTCGAGT  >  NC_002947/1532989‑1533274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑