| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,544,499 | C→G | 21.0% | intergenic (+58/+56) | mdfA → / ← ampG | multidrug MFS transporter/muropeptide permease AmpG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,544,499 | 0 | C | G | 21.0% | 46.8 / 4.1 | 23 | intergenic (+58/+56) | mdfA/ampG | multidrug MFS transporter/muropeptide permease AmpG |
| Reads supporting (aligned to +/- strand): ref base C (9/9); new base G (2/3); total (11/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCG > NC_002947/1544365‑1544643 | ggggggggcCGTGGGCGCTGGGGCCGCTGTGGTTGGGGGTGGGGGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGc < 7:252898/145‑1 (MQ=21) gggCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGttt < 6:154529/149‑1 (MQ=255) ggCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAAACCGCTCCTTCACAGGGCCGCGCCCGTTTGTTAGAGCCGGTTTAACcgc > 1:59175/1‑148 (MQ=255) gTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGGG‑ag > 2:21611/1‑132 (MQ=18) gTTTGTGGTGGGTGGGCGGCCTGGGGAAGGCGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCAGCTCCTACACAGGGCCGCGCCGCTTTGTaa > 4:295311/1‑113 (MQ=255) ccTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCG‑a > 4:262892/1‑113 (MQ=255) ccTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCG‑a < 3:262892/113‑1 (MQ=255) ggCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCg < 7:250291/149‑1 (MQ=255) tcgCTCTATAATCGGTATTGCCGGGGGCGGCGTGTTCGCGGTTAAACCGGCTCTTACACCGGGCCGGGGCGGTTTTTAGTTGGGTGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTg < 8:50930/146‑1 (MQ=255) gCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGGGGGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTgg < 5:125858/147‑1 (MQ=255) tttactgggTATTGCCGGGGCGGGCCTGTTCGCGGTTAAACCGGCTCCTACACTGGGCGGCGCCGATTTGTAGTAGCGGGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTcc < 2:189157/142‑1 (MQ=37) gcgcggTAACCCCGCCCCCACCCCCGGCCGCCGCGGTTTGGTCGGGGGGGTTAACCCGGGTAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTcc < 1:311630/143‑1 (MQ=255) cgcgGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGa > 6:274607/1‑149 (MQ=255) ggTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCcccgccc < 3:193991/135‑1 (MQ=255) ggTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCcccgccc > 4:193991/1‑135 (MQ=255) aCCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGCGCGGGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGc < 2:211829/149‑1 (MQ=255) aCCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTg > 6:175783/1‑148 (MQ=255) tACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCG‑AAGAGTCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGt < 1:228119/93‑1 (MQ=255) tACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGt > 2:228119/1‑93 (MQ=255) tACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgat > 1:112148/1‑148 (MQ=37) acaGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcag > 8:186030/1‑148 (MQ=255) aGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCa > 7:197193/1‑136 (MQ=255) aGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCa < 8:197193/136‑1 (MQ=255) cgcgccgcTTTGTAGGAGCGGGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCg > 7:78184/1‑149 (MQ=255) | TGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCG‑AAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCG > NC_002947/1544365‑1544643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |