Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,616,805 G→A 7.1% L152L (CTG→TTG)  PP_1416 ← tricarboxylate transport protein TctA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,616,8050GA 7.1% 165.4 / 2.2 56L152L (CTG→TTG) PP_1416tricarboxylate transport protein TctA
Reads supporting (aligned to +/- strand):  ref base G (24/28);  new base A (2/2);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

TGTCACCGGTGAAGCGGTACACGCCGCTGTTGGCGTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAGGCGCCGATGAACGAACTCCAGGCCGACAGCGACAGGGCAACACCGGCCAGGCCCT  >  NC_002947/1616664‑1616945
                                                                                                                                             |                                                                                                                                            
tGTCACCGGTGAAGCGGTACACGCCGCTGTTGGCGTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAg                                                                                                                                       >  6:332611/1‑149 (MQ=255)
      cGGTGAAGCGGTACACGCCGCTGTTGGCGTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTcc                                                                                                                                 >  8:133702/1‑149 (MQ=255)
             gCGGTACACGCCGCTGTTGGCGTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGAACAAAg                                                                                                                                       >  1:305289/1‑136 (MQ=255)
             gCGGTACACGCCGCTGTTGGCGTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGAACAAAg                                                                                                                                       <  2:305289/136‑1 (MQ=255)
                 tACACGCCGCTGTTGGCGTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCg                                                                                                                        >  2:138817/1‑147 (MQ=255)
                      gccgcTGTTGGCGTCGATGCCGACCGCCGAGAGGAACGGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGa                                                                                                                 >  1:307285/1‑149 (MQ=255)
                      gccgcTGTTGGCGTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGa                                                                                                                 >  6:285570/1‑149 (MQ=255)
                        cgcTGTTGGCGTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATg                                                                                                               >  5:275839/1‑149 (MQ=255)
                        cgcTGTTGGCGTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATg                                                                                                               <  6:15682/149‑1 (MQ=255)
                        cgcTGTTGGCGTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATg                                                                                                               >  3:299502/1‑149 (MQ=255)
                        cgcTGTTGGCGTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATg                                                                                                               <  7:133702/149‑1 (MQ=255)
                         gcTGTTGGCGTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCGTGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTccgc                                                                                                                               <  6:29545/132‑1 (MQ=255)
                         gcTGTTGGCGTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCGTGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTccgc                                                                                                                               >  5:29545/1‑132 (MQ=255)
                                gCGTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTT                                                                                                       <  1:138817‑M1/149‑1 (MQ=255)
                                  gTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGAt                                                                                                                <  3:191474/138‑1 (MQ=255)
                                  gTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGAt                                                                                                                >  4:191474/1‑138 (MQ=255)
                                  gTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGAGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCG                                                                                                     >  5:47659‑M1/1‑149 (MQ=255)
                                       tGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACaaa                                                                                                                                        <  7:40159/109‑1 (MQ=255)
                                       tGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACaaa                                                                                                                                        >  8:40159/1‑109 (MQ=255)
                                                   agagGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGc                                                                                                             <  7:38729/124‑1 (MQ=255)
                                                   agagGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGc                                                                                                             >  8:38729/1‑124 (MQ=255)
                                                        aaCAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCAc                                                                               <  6:257115/149‑1 (MQ=255)
                                                         aCAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCAc                                                                               >  5:54282/1‑148 (MQ=255)
                                                               cGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGcc                                                                                                            >  4:63031/1‑113 (MQ=255)
                                                               cGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGcc                                                                                                            <  3:63031/113‑1 (MQ=255)
                                                                 aTGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCa                                                                      <  3:174140/149‑1 (MQ=255)
                                                                  tGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCACCGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCCTGCCGCAg                                                                     >  2:247459/1‑149 (MQ=255)
                                                                     gagCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGtg                                                                  <  7:289023‑M2/149‑3 (MQ=255)
                                                                        cGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAcctgcggcatggtgctgttcgccccgctgctggcg                                                               <  3:162128‑M1/149‑36 (MQ=255)
                                                                        cGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGtggc                                                                <  6:245287‑M2/148‑5 (MQ=255)
                                                                         ggCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGtggcga                                                              >  4:299502‑M2/1‑143 (MQ=255)
                                                                            aaTGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAcctgcggcatggtgctgttcgccccgctgctggcgaaat                                                           <  4:147162‑M1/149‑40 (MQ=255)
                                                                            aaTGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAACACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAcctgcggcatggtgctgttcgccccgctgctggcgaaat                                                           >  5:217080‑M1/1‑110 (MQ=255)
                                                                            aaTGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAACACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAcctgcggcatggtgctgttcgccccgctgctggcgaaat                                                           >  7:161257‑M1/1‑110 (MQ=255)
                                                                              tGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAg                                                         <  8:289023/149‑1 (MQ=255)
                                                                              tGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAg                                                         <  6:47659/149‑1 (MQ=255)
                                                                              tGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAACACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAcctgcggcatggtgctgttcgccccgctgctggcgaaatgg                                                         <  6:217080‑M1/149‑42 (MQ=255)
                                                                              tGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAACACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAcctgcggcatggtgctgttcgccccgctgctggcgaaatgg                                                         <  8:161257‑M1/149‑42 (MQ=255)
                                                                                          gTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAGGCGCCGATgaac                                             <  6:275839/149‑1 (MQ=255)
                                                                                          gTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAGGCGCCGATgaac                                             <  5:245287/149‑1 (MQ=255)
                                                                                             ggttGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGGACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAGGCGCCGATgaacgaa                                          <  8:89903/147‑1 (MQ=255)
                                                                                              tttGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGtggcga                                                              <  8:145509‑M2/128‑7 (MQ=255)
                                                                                              tttGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGtggcga                                                              >  7:145509‑M2/1‑122 (MQ=255)
                                                                                                    aCCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAGGCGCCGATGAACGAACTCCAgg                                   >  3:287958/1‑149 (MQ=255)
                                                                                                     ccGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGc                                                                                 >  6:51082/1‑102 (MQ=255)
                                                                                                     ccGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGc                                                                                 <  5:51082/102‑1 (MQ=255)
                                                                                                                 cccAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAGGCGCCGATGAACGAACTCCAGGCcgacagcgacag                      <  4:287958/149‑1 (MQ=255)
                                                                                                                   cAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAGGCGCCGATGAACGAACTCCAGGCCGACAGCGACAggg                    <  5:285570/149‑1 (MQ=255)
                                                                                                                    aaCGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAGGCGCCGATgaac                                             <  5:272061/123‑1 (MQ=255)
                                                                                                                    aaCGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAGGCGCCGATgaac                                             >  6:272061/1‑123 (MQ=255)
                                                                                                                             aTGTCGAACACCATCAGAACAAAGTACTCCGCCGGGCCGACGGAGATGGCACATTTCGCCAGCAGAGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAGGCGCCGATGAACGAACTCCAGGCCGACAGCGACAGGGCAACACCGGc          <  2:307285/149‑1 (MQ=255)
                                                                                                                                 cGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAGGCGCCGATGAACGAACTCCAGGCcgacag                            <  2:82175/127‑1 (MQ=255)
                                                                                                                                 cGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAGGCGCCGATGAACGAACTCCAGGCcgacag                            >  1:82175/1‑127 (MQ=255)
                                                                                                                                  gAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAGGCGCCGATGAACGAACTCCAGGCCGACAGCGACAGGGCAACACCg            <  7:226225/142‑1 (MQ=255)
                                                                                                                                  gAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAGGCGCCGATGAACGAACTCCAGGCCGACAGCGACAGGGCAACACCg            >  8:226225/1‑142 (MQ=255)
                                                                                                                                     cacCATCAGCGCAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAGGCGCCGATGAACGAACTCCAGGCCGACAGCGACAGGGCAACACCGGCCAGGCCCt  >  8:178467/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                            
TGTCACCGGTGAAGCGGTACACGCCGCTGTTGGCGTCGATGCCGACCGCCGAGAGGAACAGGCCGATGAGAGCGGCAATGAAGGTTTTCAGTGGTTTGTCACCGGCCATGCCACCCAACGCGACGATGGCGAACACCATCAGCACAAAGTACTCCGCCGGGCCGAAGGCGATGGCCCATTTCGCCAGCAGCGGGGCGAACAGCACCATGCCGCAGGTGGCGATGAAGGCGCCGATGAACGAACTCCAGGCCGACAGCGACAGGGCAACACCGGCCAGGCCCT  >  NC_002947/1616664‑1616945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑