Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,624,963 T→C 7.5% V82A (GTG→GCG)  PP_1425 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,624,9630TC 7.5% 151.0 / 2.3 54V82A (GTG→GCG) PP_1425hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (24/25);  new base C (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTGGGCGAGCCCGCTGCCCGGGTGGTTGGCCTTTTCGGTTGTCGCCGCCTGCACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGTGGGGCGTTGGCTGGGGGAAAGCCAGTGTATACCTCAGGATGC  >  NC_002947/1624815‑1625095
                                                                                                                                                    |                                                                                                                                    
gtgtGGGCGAGCCCGCTGCCCGGGTGGTTGGCCTTTTCGGTTGTCGCCGCCTGCACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGgt                                                                                                                                      <  5:332983/149‑1 (MQ=255)
      gCGAGCCCGCTGCCCGGGTGGTTGGCCTTTTCGGTTGTCGCCGCCTGCACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAg                                                                                                                                <  5:316878/149‑1 (MQ=255)
        gAGCCCGCTGCCCGGGTGGTTGGCCTTTTCGGTTGTCGCCGCCTGGACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTc                                                                                                                              >  1:227698/1‑149 (MQ=255)
        gAGCCCGCTGCCCGGGTGGTTGGCCTTTTCGGTTGTCGCCGCCTGCACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTc                                                                                                                              >  1:223064/1‑149 (MQ=255)
           cccGCTGCCCGGGTGGTTGGCCTTTTCGGTTGTCGCCGCCTGCACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTg                                                                                                                           >  8:79227/1‑149 (MQ=255)
                ggCCCGGGTGGGTGGCCTTTTCGGTTGGCGCCGCCTGCACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCCGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCtgt                                                                                                                      <  2:252296/148‑1 (MQ=255)
                                cTTTTCGGTTGTCGCCGCCTGCACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTc                                                                                                      >  5:170688/1‑149 (MQ=255)
                                 ttttCGGTTGTCGCCGCCTGCACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCg                                                                                                     >  1:292507/1‑149 (MQ=255)
                                   ttCGGTTGTCGCCGCCTGCACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCgg                                                                                                    <  2:223064/148‑1 (MQ=255)
                                   ttCGGTTGTCGCCGCCTGCACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCgg                                                                                                    <  2:227698/148‑1 (MQ=255)
                                       gTTGTCGCCGCCTGCACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTtgtg                                                                                               >  6:37996/1‑149 (MQ=255)
                                           tCGCCGCCTGCACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCgg                                                                                           <  2:134517/149‑1 (MQ=255)
                                              ccgccTGCACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGCGTTCAGTCg                                                                                                                             >  5:330905/1‑112 (MQ=255)
                                              ccgccTGCACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGCGTTCAGTCg                                                                                                                             <  6:330905/112‑1 (MQ=255)
                                              cacggtGCAGGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGc                                                                                                                          <  3:148373/110‑1 (MQ=255)
                                              cacggtGCAGGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGc                                                                                                                          >  4:148373/6‑115 (MQ=255)
                                                 ccTGCACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAgt                                                                                     <  4:281415/149‑1 (MQ=255)
                                                         gCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGtt                                                                             <  2:292507/149‑1 (MQ=255)
                                                           ccACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAgt                                                                                     <  7:276456/139‑1 (MQ=255)
                                                           ccACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAgt                                                                                     >  8:276456/1‑139 (MQ=255)
                                                            cACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGtt                                                                                                       <  3:56088/120‑1 (MQ=255)
                                                            cACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGtt                                                                                                       >  4:56088/1‑120 (MQ=255)
                                                            cACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGcaacgcc                                                                          <  6:170688/149‑7 (MQ=255)
                                                            cACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGCGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGc                                                                           >  7:236725/1‑148 (MQ=255)
                                                                       ggCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCtggtg                                                                <  7:79227/148‑1 (MQ=255)
                                                                              ccccGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCAGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTgcg                                                        >  4:189929/1‑149 (MQ=255)
                                                                               cccGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTGCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGGGGCGTTGCCACGGCTGGTGGTTTTgcg                                                        <  2:316647/148‑1 (MQ=255)
                                                                                ccGAGGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGTGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGt                                                                                                   <  8:17299/104‑1 (MQ=255)
                                                                                ccGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGt                                                                                                   >  7:17299/1‑104 (MQ=255)
                                                                                ccGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTAGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCt                                                      >  3:215899/1‑149 (MQ=255)
                                                                                  gACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCttt                                                    <  5:325573/149‑1 (MQ=255)
                                                                                      tATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGc                                                >  8:184586/1‑149 (MQ=255)
                                                                                       aTCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCtggt                                                                 <  7:160281/131‑1 (MQ=255)
                                                                                       aTCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCtggt                                                                 >  8:160281/1‑131 (MQ=255)
                                                                                          cTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGCGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGt                                            <  8:236725/149‑1 (MQ=255)
                                                                                             ttGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGTggg                                         >  7:237189/1‑149 (MQ=255)
                                                                                              tGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTAGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCGACGGCTGGTGGTTTTGCGCTTTGAGCGGGTggg                                         <  4:215899/148‑1 (MQ=255)
                                                                                              agccccAGGAGCCTGCAGTTGCCGGCCTCCCCCGGGAGGGGCGGGGCTGGCAGGGGTTCAGTCGCCCCTCTGGGGGGCAGCTGGCTCGGTTGTCCCGGGACAGTGTGGCGTTGCCCCGGCTGGTGGTTTGGCGGTTTGAGCGGGCgggg                                        <  8:85029/146‑1 (MQ=255)
                                                                                               gACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAg                                                 >  8:32096/1‑139 (MQ=255)
                                                                                               gACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAg                                                 <  7:32096/139‑1 (MQ=255)
                                                                                                    aTGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGTGGGGCGTTgg                                  <  8:237189/149‑1 (MQ=255)
                                                                                                         cATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGTGGGGCGTTgg                                  >  6:61942/1‑144 (MQ=255)
                                                                                                         cATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGTGGGGCGTTgg                                  <  5:61942/144‑1 (MQ=255)
                                                                                                           tGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGTGGGGCGTTGGCTgggg                            >  2:29879/1‑148 (MQ=255)
                                                                                                                ttgtCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGTGGGGCGTTGGCTggg                             <  5:166512/142‑1 (MQ=255)
                                                                                                                ttgtCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGTGGGGCGTTGGCTggg                             >  6:166512/1‑142 (MQ=255)
                                                                                                                      gCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTAGCAGAGGGTTCGGTTGTGCCGGGACAGTGTGGAGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGTGGGGCGTTGGCTGGGGGAAAGCCAgtg                 >  1:80804/1‑148 (MQ=255)
                                                                                                                             ccGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGTGGGGCGTTGGCTGGGGGAAAGCCAgt                  <  5:71016/140‑1 (MQ=255)
                                                                                                                             ccGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGTGGGGCGTTGGCTGGGGGAAAGCCAgt                  >  6:71016/1‑140 (MQ=255)
                                                                                                                              cGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGTGGGGCGTTGGCTGGGGGAAAGCCAGTGTATACCt          >  2:290593/1‑147 (MQ=255)
                                                                                                                                   tgtgCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGt                                            <  3:15587/108‑1 (MQ=255)
                                                                                                                                   tgtgCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGt                                            >  4:15587/1‑108 (MQ=255)
                                                                                                                                   tgtgCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGTGGGGCGTTGGCTGGGGGAAAGCCAgt                  <  2:38062/134‑1 (MQ=255)
                                                                                                                                   tgtgCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGTGGGGCGTTGGCTGGGGGAAAGCCAgt                  >  1:38062/1‑134 (MQ=255)
                                                                                                                                     tgCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGTGGGGCGTTGGCTGGGGGAAAGCCAGTGTATACCTCAGGATgc  <  5:37996/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                    
GTGTGGGCGAGCCCGCTGCCCGGGTGGTTGGCCTTTTCGGTTGTCGCCGCCTGCACGGCCCACGCTGGCTGGGCCATTCCCCGACGTATCCTGTTGACCCATGAGCATGCTGTTGTCGGCCTGCGCCGTGATGTGCGGGGCTGGCAGGTGTTCAGTCGTGCCTGTGGGTGGCAGCGGGTTCGGTTGTGCCGGGACAGTGTGGCGTTGCCACGGCTGGTGGTTTTGCGCTTTGAGCGGGTGGGGCGTTGGCTGGGGGAAAGCCAGTGTATACCTCAGGATGC  >  NC_002947/1624815‑1625095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑