Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,631,095	C→A	11.8%	intergenic (+29/‑152)	PP_1430 → / → lepA	DegP‑like serine endoprotease/elongation factor 4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,631,095	0	C	A	11.8%	189.8 / 12.9	67	intergenic (+29/‑152)	PP_1430/lepA	DegP‑like serine endoprotease/elongation factor 4
Reads supporting (aligned to +/- strand):  ref base C (29/30);  new base A (4/4);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

GTTCGGCCAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAGT  >  NC_002947/1630951‑1631238                                                                                                                                                 |                                                                                                                                                 gTTCGGCCAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCt                                                                                                                                              <  6:34857/149‑1 (MQ=255)  ttCGGCCAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTg                                                                                                                                             <  2:73870/149‑1 (MQ=255)  atccgCCAAGGAATTCACCGAAATCGCCAAGACACTGACGAAGAATCGTTCCGTGTCGATCCGCATACTGAGTCAGGGCCGTTCAAGCTTCATCACTTTCAAGCTAGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTg                                                                                                                                             >  6:20277/5‑149 (MQ=255)        cAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCttt                                                                                                                                       <  3:86038/149‑1 (MQ=255)        cAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCttt                                                                                                                                       >  6:299060/1‑149 (MQ=255)        cAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCttt                                                                                                                                       >  7:87790/1‑149 (MQ=255)        cAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCttt                                                                                                                                       >  2:237239/1‑149 (MQ=255)         aaGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCCGAAGCTGCCCttt                                                                                                                                       <  1:120859/148‑1 (MQ=255)         aaGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCCGAAGCTGCCCttt                                                                                                                                       >  2:120859/1‑148 (MQ=255)          aGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCttttt                                                                                                                                     <  7:89264/149‑1 (MQ=255)           ggAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTc                                                                                                                                    <  2:103801/149‑1 (MQ=255)             aaTTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCAAAAGGGCAGCCGAAGCTGCCCtttcgcct                                                                                                                                  <  5:123714/149‑6 (MQ=255)              aTTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATg                                                                                                                                 <  6:10615/149‑1 (MQ=255)               ttCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGa                                                                                                                                <  8:87790/149‑1 (MQ=255)               ttCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCCGAAGCTGCCCtttcgccttg                                                                                                                                <  2:175759/149‑8 (MQ=255)                  aCCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATc                                                                                                                             <  5:299060/149‑1 (MQ=255)                    cGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCtt                                                                                                                           >  6:266993/1‑149 (MQ=255)                             cAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑c                                                                                                                 >  2:18371/1‑149 (MQ=255)                              aaGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAAtt                                                                                                                    <  7:123753/146‑1 (MQ=255)                              aaGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAAtt                                                                                                                    >  8:123753/1‑146 (MQ=255)                                 gAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTca                                                                                                                         <  4:162990/138‑1 (MQ=255)                                 gAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTca                                                                                                                         >  3:162990/1‑138 (MQ=255)                                       acGAAGAATCGTTCCGTGTCGATGCTCGTACTGCGTCAGGTCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTAGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGAt                                                                                                        <  8:9345/147‑1 (MQ=255)                                          aagaaTCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCt                                                                                                    >  5:198468/1‑149 (MQ=255)                                              aTCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTACATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTATTTCATGAATCTTCACAATTG‑CCGGAATGATTGCtgtg                                                                                                 >  6:135782/1‑148 (MQ=255)                                               tCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAAATG‑CCGGAATGATTGCTgtgt                                                                                                >  7:220399/1‑148 (MQ=255)                                               tCGTTCCGGGTCGATGCGCGTACTGCGTCAGGGCCGTGCCATCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTa                                                                                               <  7:294780/149‑1 (MQ=255)                                                      gtgtCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCAAAATTG‑CAGGAATGATTGCTGTGTACATTCg                                                                                         >  6:316520/1‑148 (MQ=255)                                                      gtgtCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCAAAATTG‑ACTGAATGATTGCTGTGTACAGTCg                                                                                         >  6:311947/1‑148 (MQ=255)                                                       tgtCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGa                                                                                       <  4:33368/149‑1 (MQ=255)                                                              gcgcGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAgg                                                                                 >  5:77818/1‑148 (MQ=255)                                                              gcgcGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAgg                                                                                 >  4:298715/1‑148 (MQ=255)                                                                   tACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAAtctc                                                                           <  6:77818/149‑1 (MQ=255)                                                                      tGCGTCAGGGCCGTGCCAGCTTCATCACTTTTAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCa                                                                        >  7:77525/1‑149 (MQ=255)                                                                      tGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCa                                                                        <  3:298715/149‑1 (MQ=255)                                                                        cGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGCCAGAGGAATCTCCCAt                                                                       >  6:101769/1‑148 (MQ=255)                                                                          tCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAAc                                                                    >  4:163943/1‑149 (MQ=255)                                                                              ggCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTGCCCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCca                                                                 <  1:237239/149‑1 (MQ=255)                                                                                      cAGCTTCATCACTTTCAATCTTGATGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCACTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTaa                                                        <  5:316520/149‑1 (MQ=255)                                                                                      cAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTaa                                                        <  2:100701/149‑1 (MQ=255)                                                                                      cAGCTTAATCACTTTCAAGCTTTCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCACTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTaa                                                        <  5:311947/149‑1 (MQ=255)                                                                                              tcaCTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATGTCACAAAACCCAGCAGCTTAAGGTACAAt                                                >  3:271324/1‑149 (MQ=255)                                                                                                     cAAGCTTGATGAATAAGCAGTTTCGCTAGGCTAAAGGGCAGCTTCGGCTGCCCTTTTTCATTAATGTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGTCAGAGGAATCTCCCATAACCCAGCAGGTTAAGGTACAATTCCCgg                                          <  5:20277/148‑1 (MQ=255)                                                                                                      aaGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCt                                        >  6:56971/1‑149 (MQ=255)                                                                                                        gCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTAt                                      >  7:232604/1‑149 (MQ=255)                                                                                                               gAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑GCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTcgg                               >  8:204968/1‑149 (MQ=255)                                                                                                               gAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTcgg                               >  3:79413/1‑149 (MQ=255)                                                                                                                aaTAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTcg                                <  7:99915/147‑1 (MQ=255)                                                                                                                aaTAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTcg                                <  6:198468/147‑1 (MQ=255)                                                                                                                aaTAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTATTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTcg                                <  5:135782/147‑1 (MQ=255)                                                                                                                     gCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCggcgggcg                         >  4:194697/1‑149 (MQ=255)                                                                                                                      cAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTcggcg                             >  4:902/1‑144 (MQ=255)                                                                                                                      cAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTcggcg                             <  3:902/144‑1 (MQ=255)                                                                                                                      cAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGCCTATTTTTCGGCGGGCGt                        >  4:37581/1‑149 (MQ=255)                                                                                                                       aGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTc                       <  5:56971/149‑1 (MQ=255)                                                                                                                              cAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCg                >  5:39389/1‑149 (MQ=255)                                                                                                                                 tgaaaaaGGGCAGCCGAAGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAg             >  7:126876/5‑149 (MQ=255)                                                                                                                                  gCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGc            <  4:79413/149‑1 (MQ=255)                                                                                                                                   aaaaaGGGCAGCCGAAGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGcc           >  3:177990/3‑149 (MQ=255)                                                                                                                                    gAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTc                       >  3:293553/1‑136 (MQ=255)                                                                                                                                    gAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTc                       <  4:293553/136‑1 (MQ=255)                                                                                                                                    gAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCt          <  3:37581/149‑1 (MQ=255)                                                                                                                                    aaaaGGGCAGCCGAAGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCt          >  6:135376/2‑149 (MQ=255)                                                                                                                                        gggCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGt                        >  4:148377/1‑131 (MQ=255)                                                                                                                                        gggCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGt                        <  3:148377/131‑1 (MQ=255)                                                                                                                                           cAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAg   <  8:232604/149‑1 (MQ=255)                                                                                                                                            aGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAgt  <  3:194697/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                 GTTCGGCCAAGGAATTCACCGAAATCGCCAAGGAACTGCCGAAGAATCGTTCCGTGTCGATGCGCGTACTGCGTCAGGGCCGTGCCAGCTTCATCACTTTCAAGCTTGCTGAATAAGCAGTTTCGCAAGGCGAAAGGGCAGCTTCGGCTGCCCTTTTTCATGAATCTTCACAATTG‑CCGGAATGATTGCTGTGTACAGTCGGACAGAGGAATCTCCCATAACCCAGCAGCTTAAGGTACAATTCCCGGCTATTTTTCGGCGGGCGTCCGGCTCGCAGCCTTTTCGAGT  >  NC_002947/1630951‑1631238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑