Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,665,258 T→A 12.0% I204F (ATC→TTC)  PP_1459 ← CBS domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,665,2580TA12.0% 171.5 / 11.3 65I204F (ATC→TTC) PP_1459CBS domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (29/28);  new base A (4/4);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGCTTCCAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAGTCGTCGGCCTCCTGC  >  NC_002947/1665113‑1665406
                                                                                                                                                 |                                                                                                                                                    
aGCGCTTCCAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGAGCCGATCGAACg                                                                                                                                                    >  4:291057/1‑148 (MQ=255)
       ccAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCtcgtc                                                                                                                                             >  4:104763/1‑148 (MQ=255)
        cAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGAGCCGATCGAACGCATCGTCGa                                                                                                                                           <  3:291057/149‑1 (MQ=255)
        cAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGAGCCGATCGAACGCATCGTCGa                                                                                                                                           <  6:146076/149‑1 (MQ=255)
         aGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCATCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGCCGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAg                                                                                                                                          >  1:32161/1‑149 (MQ=255)
          gggTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGAGCCGATCGAACGCATCGTCGAgc                                                                                                                                         <  3:157555/149‑2 (MQ=255)
           ggTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGt                                                                                                                                        <  5:232181/149‑1 (MQ=255)
               aGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGAt                                                                                                                                    <  2:50728/149‑1 (MQ=255)
               aGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGAGCCGATCGAACGCATCGTCGAgcagctg                                                                                                                                    <  6:67885/149‑7 (MQ=38)
                 cTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGc                                                                                                                                  >  5:282505/1‑149 (MQ=255)
                     gCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATc                                                                                                                              <  6:282505/149‑1 (MQ=255)
                      ccTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATcc                                                                                                                             <  6:44849/149‑1 (MQ=255)
                                  ggTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTgcgc                                                                                                                 <  3:104763/149‑1 (MQ=255)
                                       cTGATCAGCTTGGTGTGGAGGATCGCTTCGACCTGGTTGATGGCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCAc                                                                                                            <  7:45490/149‑1 (MQ=255)
                                         gATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCAc                                                                                                            >  2:54419/1‑147 (MQ=255)
                                               cTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGt                                                                                                    >  7:276453/1‑149 (MQ=255)
                                                  ggTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGt                                                                                                 >  7:252983/1‑149 (MQ=255)
                                                   gtgtTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACTATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGtt                                                                                                <  8:276453/149‑1 (MQ=255)
                                                        gAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACgg                                                                                           >  6:174856/1‑149 (MQ=255)
                                                         aGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGt                                                                                          >  7:174271/1‑149 (MQ=255)
                                                               tcTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCtt                                                                                    <  7:195385/148‑1 (MQ=255)
                                                                 ttCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCtt                                                                                    >  8:195385/1‑147 (MQ=255)
                                                                     aCCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAg                                                                              >  2:230446/1‑149 (MQ=255)
                                                                      ccTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGc                                                                             <  3:284223/149‑1 (MQ=255)
                                                                       cTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGc                                                                             >  7:21979/1‑148 (MQ=255)
                                                                       cTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGc                                                                             <  8:21979/148‑1 (MQ=255)
                                                                       cTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCg                                                                            >  4:229311/1‑149 (MQ=255)
                                                                          gTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGAt                                                                                       >  5:133374/1‑135 (MQ=255)
                                                                          gTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGAt                                                                                       <  6:133374/135‑1 (MQ=255)
                                                                                gTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTg                                                                                         >  6:147389/1‑127 (MQ=255)
                                                                                gTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTg                                                                                         <  5:147389/127‑1 (MQ=255)
                                                                                  cGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGcc                                                                  <  8:174271/148‑1 (MQ=255)
                                                                                   gTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGt                                                                <  8:252983/149‑1 (MQ=255)
                                                                                      gtgGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGt                                                                                  >  4:47644/1‑128 (MQ=255)
                                                                                      gtgGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGt                                                                                  <  3:47644/128‑1 (MQ=255)
                                                                                         gTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAg                                                            >  7:286817/1‑147 (MQ=255)
                                                                                               cGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCAt                                                                                                                               >  8:9693/1‑74 (MQ=255)
                                                                                               cGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCAt                                                                                                                               <  7:9693/74‑1 (MQ=255)
                                                                                                 gCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTgg                                                  >  7:262231/1‑149 (MQ=255)
                                                                                                      cGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTTGCGAgg                                             <  5:174856/149‑1 (MQ=255)
                                                                                                        ggTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGgcg                                           <  1:230446/149‑1 (MQ=255)
                                                                                                         gtgtGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGgcgc                                          >  6:35232/1‑149 (MQ=255)
                                                                                                            tgGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTc                                       <  5:253692/149‑1 (MQ=255)
                                                                                                            tgGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTc                                       <  1:54419/149‑1 (MQ=255)
                                                                                                                 gcTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGc                                                 >  6:217222/1‑134 (MQ=255)
                                                                                                                 gcTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGc                                                 <  5:217222/134‑1 (MQ=255)
                                                                                                                  cTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCTTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTg                                 <  8:286817/149‑1 (MQ=255)
                                                                                                                    gACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGt                                >  3:107649/1‑148 (MQ=255)
                                                                                                                         tCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATcc                          >  2:160388/1‑149 (MQ=255)
                                                                                                                            gctgctCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTg                                    <  8:26785/136‑1 (MQ=255)
                                                                                                                            gctgctCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTg                                    >  7:26785/1‑136 (MQ=255)
                                                                                                                            gctgctCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCa                       >  8:298609/1‑149 (MQ=255)
                                                                                                                                ctCGACGATGAGTTCGATCTGCTCGTCGAGGTTGATGCCATCCTCTTCGTTGCGCCTCACCAGGATGGCGTTGTCGGTTATCTTGTAGAGCGTGTGCAGGCCGTAGAGCATACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATc                     <  2:32161/147‑1 (MQ=255)
                                                                                                                                ctCGACGAGCCGATCGAACGCATCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCg                    >  1:12096/1‑148 (MQ=255)
                                                                                                                                 tCGACGAGCCGCTCGAACGCATCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGAACGAGATCTAGTCCAGCGACTCCAGGCCGTCTAGCAGACCCTGGCGAGGAGCTTCGTGCTGTTCATCGTCAACGa                   >  2:252867/1‑148 (MQ=255)
                                                                                                                                 tCGACGAGCCGATCGAACGCATCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCCTCCTCATCg                    >  8:299491/1‑147 (MQ=255)
                                                                                                                                  cgacgaTGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAg                 >  6:288778/1‑149 (MQ=255)
                                                                                                                                  cgacgaTGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAg                 >  5:66377/1‑149 (MQ=255)
                                                                                                                                  cgacgaTGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAg                 >  8:54545/1‑149 (MQ=255)
                                                                                                                                   gacgaTGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAg                 <  5:288778/148‑1 (MQ=255)
                                                                                                                                       aTGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAgtcgtc            <  1:124547/149‑1 (MQ=255)
                                                                                                                                        tGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGgc                                            <  6:305635/116‑1 (MQ=255)
                                                                                                                                        tGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGgc                                            >  5:305635/1‑116 (MQ=255)
                                                                                                                                           gTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAGTCGTCGGcc        >  2:11228/1‑149 (MQ=255)
                                                                                                                                               gATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGTCGCTTCGTTCTGTTCATCCTCATCGAAGTCGTCGGcctcct    <  5:35232/149‑1 (MQ=255)
                                                                                                                                                 tCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAGTCGTCGGCCTCCTGc  <  8:98920/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AGCGCTTCCAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAGTCGTCGGCCTCCTGC  >  NC_002947/1665113‑1665406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑