Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,665,262 C→A 10.8% E202D (GAG→GAT)  PP_1459 ← CBS domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,665,2620CA10.8% 186.7 / 12.2 65E202D (GAG→GAT) PP_1459CBS domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (29/29);  new base A (3/4);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01

CCAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAGTCGTCGGCCTCCTGC  >  NC_002947/1665120‑1665406
                                                                                                                                              |                                                                                                                                                
ccAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCtcgtc                                                                                                                                             >  4:104763/1‑148 (MQ=255)
 cAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGAGCCGATCGAACGCATCGTCGa                                                                                                                                           <  6:146076/149‑1 (MQ=255)
 cAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGAGCCGATCGAACGCATCGTCGa                                                                                                                                           <  3:291057/149‑1 (MQ=255)
  aGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCATCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGCCGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAg                                                                                                                                          >  1:32161/1‑149 (MQ=255)
   gggTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGAGCCGATCGAACGCATCGTCGAgc                                                                                                                                         <  3:157555/149‑2 (MQ=255)
    ggTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGt                                                                                                                                        <  5:232181/149‑1 (MQ=255)
        aGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGAt                                                                                                                                    <  2:50728/149‑1 (MQ=255)
        aGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGAGCCGATCGAACGCATCGTCGAgcagctg                                                                                                                                    <  6:67885/149‑7 (MQ=38)
          cTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGc                                                                                                                                  >  5:282505/1‑149 (MQ=255)
              gCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATc                                                                                                                              <  6:282505/149‑1 (MQ=255)
               ccTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATcc                                                                                                                             <  6:44849/149‑1 (MQ=255)
                           ggTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTgcgc                                                                                                                 <  3:104763/149‑1 (MQ=255)
                                cTGATCAGCTTGGTGTGGAGGATCGCTTCGACCTGGTTGATGGCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCAc                                                                                                            <  7:45490/149‑1 (MQ=255)
                                  gATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCAc                                                                                                            >  2:54419/1‑147 (MQ=255)
                                        cTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGt                                                                                                    >  7:276453/1‑149 (MQ=255)
                                           ggTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGt                                                                                                 >  7:252983/1‑149 (MQ=255)
                                            gtgtTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACTATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGtt                                                                                                <  8:276453/149‑1 (MQ=255)
                                                 gAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACgg                                                                                           >  6:174856/1‑149 (MQ=255)
                                                  aGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGt                                                                                          >  7:174271/1‑149 (MQ=255)
                                                        tcTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCtt                                                                                    <  7:195385/148‑1 (MQ=255)
                                                          ttCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCtt                                                                                    >  8:195385/1‑147 (MQ=255)
                                                              aCCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAg                                                                              >  2:230446/1‑149 (MQ=255)
                                                               ccTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGc                                                                             <  3:284223/149‑1 (MQ=255)
                                                                cTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGc                                                                             <  8:21979/148‑1 (MQ=255)
                                                                cTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGc                                                                             >  7:21979/1‑148 (MQ=255)
                                                                cTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCg                                                                            >  4:229311/1‑149 (MQ=255)
                                                                   gTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGAt                                                                                       <  6:133374/135‑1 (MQ=255)
                                                                   gTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGAt                                                                                       >  5:133374/1‑135 (MQ=255)
                                                                         gTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTg                                                                                         <  5:147389/127‑1 (MQ=255)
                                                                         gTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTg                                                                                         >  6:147389/1‑127 (MQ=255)
                                                                           cGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGcc                                                                  <  8:174271/148‑1 (MQ=255)
                                                                            gTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGt                                                                <  8:252983/149‑1 (MQ=255)
                                                                               gtgGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGt                                                                                  >  4:47644/1‑128 (MQ=255)
                                                                               gtgGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGt                                                                                  <  3:47644/128‑1 (MQ=255)
                                                                                  gTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAg                                                            >  7:286817/1‑147 (MQ=255)
                                                                                        cGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCAt                                                                                                                               >  8:9693/1‑74 (MQ=255)
                                                                                        cGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCAt                                                                                                                               <  7:9693/74‑1 (MQ=255)
                                                                                          gCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTgg                                                  >  7:262231/1‑149 (MQ=255)
                                                                                               cGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTTGCGAgg                                             <  5:174856/149‑1 (MQ=255)
                                                                                                 ggTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGgcg                                           <  1:230446/149‑1 (MQ=255)
                                                                                                  gtgtGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGgcgc                                          >  6:35232/1‑149 (MQ=255)
                                                                                                     tgGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTc                                       <  5:253692/149‑1 (MQ=255)
                                                                                                     tgGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTc                                       <  1:54419/149‑1 (MQ=255)
                                                                                                          gcTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGc                                                 >  6:217222/1‑134 (MQ=255)
                                                                                                          gcTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGc                                                 <  5:217222/134‑1 (MQ=255)
                                                                                                           cTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCTTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTg                                 <  8:286817/149‑1 (MQ=255)
                                                                                                             gACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGt                                >  3:107649/1‑148 (MQ=255)
                                                                                                                  tCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATcc                          >  2:160388/1‑149 (MQ=255)
                                                                                                                     gctgctCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTg                                    <  8:26785/136‑1 (MQ=255)
                                                                                                                     gctgctCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTg                                    >  7:26785/1‑136 (MQ=255)
                                                                                                                     gctgctCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCa                       >  8:298609/1‑149 (MQ=255)
                                                                                                                         ctCGACGATGAGTTCGATCTGCTCGTCGAGGTTGATGCCATCCTCTTCGTTGCGCCTCACCAGGATGGCGTTGTCGGTTATCTTGTAGAGCGTGTGCAGGCCGTAGAGCATACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATc                     <  2:32161/147‑1 (MQ=255)
                                                                                                                         ctCGACGAGCCGATCGAACGCATCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCg                    >  1:12096/1‑148 (MQ=255)
                                                                                                                          tCGACGAGCCGCTCGAACGCATCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGAACGAGATCTAGTCCAGCGACTCCAGGCCGTCTAGCAGACCCTGGCGAGGAGCTTCGTGCTGTTCATCGTCAACGa                   >  2:252867/1‑148 (MQ=255)
                                                                                                                          tCGACGAGCCGATCGAACGCATCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCCTCCTCATCg                    >  8:299491/1‑147 (MQ=255)
                                                                                                                           cgacgaTGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAg                 >  6:288778/1‑149 (MQ=255)
                                                                                                                           cgacgaTGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAg                 >  5:66377/1‑149 (MQ=255)
                                                                                                                           cgacgaTGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAg                 >  8:54545/1‑149 (MQ=255)
                                                                                                                            gacgaTGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAg                 <  5:288778/148‑1 (MQ=255)
                                                                                                                                aTGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAgtcgtc            <  1:124547/149‑1 (MQ=255)
                                                                                                                                 tGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGgc                                            <  6:305635/116‑1 (MQ=255)
                                                                                                                                 tGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGgc                                            >  5:305635/1‑116 (MQ=255)
                                                                                                                                    gTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAGTCGTCGGcc        >  2:11228/1‑149 (MQ=255)
                                                                                                                                        gATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGTCGCTTCGTTCTGTTCATCCTCATCGAAGTCGTCGGcctcct    <  5:35232/149‑1 (MQ=255)
                                                                                                                                          tCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAGTCGTCGGCCTCCTGc  <  8:98920/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
CCAGGGTCAGCTCGGCCTTGGGCAGCAGGTGGCTGATCAGCTTGGTGTTGAGGATCGCTTCGACCTGGTTGATGTCGTTGTGGTAGACCGGCAGGCGGGTGTGGCGGCTGACGATCAGCTGCTCGACGATGCGTTCGATCGGCTCGTCGAGGTTGATGCCATCCACTTCGTTGCGCGGCACCAGGATGTCGTTGACGGTGATCTTGTCCAGCGACTGCAGGCCGTCGAGCAGACCCTGGCGAGGCGCTTCGTGCTGTTCATCCTCATCGAAGTCGTCGGCCTCCTGC  >  NC_002947/1665120‑1665406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑