Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 184,283 T→C 9.6% N94D (AAC→GAC) ‡ PP_0160 ← ferrioxamine receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947184,2830TC 9.6% 139.9 / 5.7 52N94D (AAC→GAC) ‡PP_0160ferrioxamine receptor
Reads supporting (aligned to +/- strand):  ref base T (23/24);  new base C (3/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

ATGTCGGTGTCTGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCGCAACGGAAAGTTG  >  NC_002947/184135‑184430
                                                                                                                                                    |                                                                                                                                                   
aTGTCGGTGTCTGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGtt                                                                                                                                                     >  4:279965/1‑149 (MQ=255)
    cGGTGTCTGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGtt                                                                                                                                                     <  1:123383/145‑1 (MQ=255)
    cGGTGTCTGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGtt                                                                                                                                                     >  2:123383/1‑145 (MQ=255)
       tgtCTGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATg                                                                                                                                               >  3:90068/1‑148 (MQ=255)
         tCTGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCATTCCAACGGACTGCAg                                                                                                                                            <  6:134486/149‑1 (MQ=255)
           tGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGc                                                                                                                                          >  4:95281/1‑149 (MQ=255)
           tGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGc                                                                                                                                          >  7:188307/1‑149 (MQ=255)
            ggCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCATTCCAACGGACTGCAgg                                                                                                                                           <  4:115576/147‑1 (MQ=255)
            ggCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCATTCCAACGGACTGCAgg                                                                                                                                           >  3:115576/1‑147 (MQ=255)
                   ccAGGGCGGCGTTCTGACGCTGTGCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGt                                                                                                                                   >  7:176405/1‑148 (MQ=255)
                          ggcgTTCTGACGCTGTGCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAg                                                                                                                           <  6:130462/149‑1 (MQ=255)
                          ggcgTTCTGACGCTGTGCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCAGTCAGCTGAg                                                                                                                           <  8:188307/149‑1 (MQ=255)
                                     gCTGTGCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGAt                                                                                                                <  3:279965/149‑1 (MQ=255)
                                       tgtgCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAg                                                                                                              >  8:118417/1‑149 (MQ=255)
                                         tgCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAggg                                                                                                            <  6:97288/149‑1 (MQ=255)
                                         tgCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAgg                                                                                                             <  8:176405/148‑1 (MQ=255)
                                           ccTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGt                                                                                                           <  8:254755/148‑1 (MQ=255)
                                                        gTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGgc                                                                                             <  3:95281/149‑1 (MQ=255)
                                                            cGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCCGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGgcgag                                                                                          <  2:192203/148‑1 (MQ=255)
                                                             gTCCCCCCTGGCTGACGGCTTCCAGTTCGCTGCCATTAAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGgcgagc                                                                                         >  3:243749/1‑148 (MQ=255)
                                                                 ccccTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATtg                                                                                    >  5:128742/1‑149 (MQ=255)
                                                                  cccTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGgcgagc                                                                                         <  2:81061/143‑1 (MQ=255)
                                                                  cccTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGgcgagc                                                                                         >  1:81061/1‑143 (MQ=255)
                                                                       gCTGCCGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTg                                                                               <  7:253428/148‑1 (MQ=255)
                                                                       gCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGtt                                                                                <  4:297637/147‑1 (MQ=255)
                                                                           aCGGCTTCCATTCCGCTGCCATGCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGGGATCCCGGCCTGGCGAGCGATTGTGTTGAGCa                                                                           <  4:196162/148‑1 (MQ=255)
                                                                              gCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTc                                                                       <  6:44584/149‑1 (MQ=255)
                                                                                ttCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCgg                                                                     >  3:284337/1‑149 (MQ=255)
                                                                                      tCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCGCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGtt                                                               <  4:90068/149‑1 (MQ=255)
                                                                                          cTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGAAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGc                                                            >  5:45286/1‑148 (MQ=255)
                                                                                           tGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGtt                                                                                <  3:174901/127‑1 (MQ=255)
                                                                                           tGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGtt                                                                                >  4:174901/1‑127 (MQ=255)
                                                                                             ccATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGc                                                        >  3:301806/1‑149 (MQ=255)
                                                                                               aTTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTg                                                      >  5:10348/1‑149 (MQ=255)
                                                                                                 tCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGtt                                                                                <  5:221921/121‑1 (MQ=255)
                                                                                                 tCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGtt                                                                                >  6:221921/1‑121 (MQ=255)
                                                                                                 tCAGCAACTGGCGCAGCGACTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTgg                                                     >  4:80180/1‑148 (MQ=255)
                                                                                                    gCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTAt                                                  >  1:114773/1‑148 (MQ=255)
                                                                                                               aGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGGCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTg                                      <  4:243749/149‑1 (MQ=255)
                                                                                                                     tGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCAc                                >  3:280011/1‑149 (MQ=255)
                                                                                                                       aTCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACtt                              <  4:301806/149‑1 (MQ=255)
                                                                                                                           gCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGc                          >  1:23673/1‑149 (MQ=255)
                                                                                                                            cTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCt                         <  2:23673/149‑1 (MQ=255)
                                                                                                                            cTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCt                         <  6:10348/149‑1 (MQ=255)
                                                                                                                               tgtACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCtgtg                      <  6:128742/149‑1 (MQ=255)
                                                                                                                               tgtACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCtgt                       <  7:118417/148‑1 (MQ=255)
                                                                                                                                gtACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTgtgt                     >  8:297506/1‑149 (MQ=255)
                                                                                                                                 tACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTc                    >  2:46993/1‑149 (MQ=255)
                                                                                                                                      ccccTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCg               >  4:24092/1‑149 (MQ=255)
                                                                                                                                      ccccTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCg               <  3:24092/149‑1 (MQ=255)
                                                                                                                                      ccccTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCg               >  1:134449/1‑149 (MQ=255)
                                                                                                                                        ccTGCATTCCAACGGACTGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGGCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCGc              >  4:90305/1‑148 (MQ=255)
                                                                                                                                        ccTGCATTCCAACGGACTGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCGc              >  1:208014/1‑148 (MQ=255)
                                                                                                                                        ccTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCGc              >  4:266442/1‑148 (MQ=255)
                                                                                                                                               tCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCGCAACGGaaa      <  3:111363/149‑1 (MQ=255)
                                                                                                                                                   ttGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCGCAACGGAAAGTTg  >  3:297496/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATGTCGGTGTCTGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCAGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCGCAACGGAAAGTTG  >  NC_002947/184135‑184430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑