Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,736,751 T→A 10.3% N297Y (AAC→TAC)  dapD ← 2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,736,7510TA10.3% 102.1 / 4.0 39N297Y (AAC→TAC) dapD2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base T (17/18);  new base A (2/2);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

CAGTTGTGAGCGTGCAGCGCTTCGTTCAGCTCGATGGCCGACTTGTGGGTCTTGCATTCTACCGCGCCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAGGCAGCCCTCGCCGACTTTGATGACGATGTTGCCGCCAC  >  NC_002947/1736606‑1736897
                                                                                                                                                 |                                                                                                                                                  
cAGTTGTGAGCGTGCAGCGCTTCGTTCAGCTCGATGGCCGACTTGTGGGTCTTGCATTCTACCGCGCCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCgttgtt                                                                                                                                                 >  4:127843/1‑149 (MQ=255)
 aGTTGTGAGCGTGCAGCGCTTCGTTCAGCTCGATGGCCGACTTGTGGGTCTTGCATTCTACCGCGCCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTtc                                                                                                                                                >  5:278243/1‑149 (MQ=255)
   ttGTGAGCGTGCAGCGCTTCGTTCAGCTCGATGGCCGACTTGTGGGTCTTGCATTCTACCGCGCCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTtctc                                                                                                                                              <  4:89749/149‑1 (MQ=255)
             gCAGCGCTTCGTTCAGCTCGATGGCCGACTTGTGGGTCTTGCATTCTACCGCGCCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAgg                                                                                                                                    >  3:25604/1‑149 (MQ=255)
                         tCAGCTCGATGGCCGACTTGTGGGTCTTGCATTCTACCGCGCCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCgttgt                                                                                                                                                  >  8:96954/1‑123 (MQ=255)
                         tCAGCTCGATGGCCGACTTGTGGGTCTTGCATTCTACCGCGCCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCgttgt                                                                                                                                                  <  7:96954/123‑1 (MQ=255)
                           aGCTCGATGGCCGACTTGTGGGTCTTGCATTCTACCGCGCCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGc                                                                                                                       <  6:278243/148‑1 (MQ=255)
                                gATGGCCGACTTGTGGGTCTTGCATTCTACCGCGCCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGCCCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCgg                                                                                                                 >  8:108347/1‑149 (MQ=255)
                                        aCTTGTGGGTCTTGCATTCTACCGCGCCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTg                                                                                                                           >  1:321631/1‑131 (MQ=255)
                                        aCTTGTGGGTCTTGCATTCTACCGCGCCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTg                                                                                                                           <  2:321631/131‑1 (MQ=255)
                                                          cTACCGCGCCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGGCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGgt                                                                                        <  4:25604/148‑1 (MQ=255)
                                                             ccGCGCCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGAGCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTg                                                                                    <  7:117310/149‑1 (MQ=255)
                                                             ccGCGCCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGgtg                                                                                       <  7:108347/146‑1 (MQ=255)
                                                                cgcCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCgg                                                                                 <  8:187850/149‑1 (MQ=255)
                                                                 gcCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGt                                                                                >  7:239868/1‑149 (MQ=255)
                                                                                gcggcgGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAatac                                                                 >  6:103686/1‑149 (MQ=255)
                                                                                gcggcgGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTAGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAatac                                                                 >  4:187949/1‑149 (MQ=255)
                                                                                gcggcgGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTAGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAatac                                                                 >  4:191865/1‑149 (MQ=255)
                                                                                                tcggtcTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGagggc                                                 >  3:67313‑M1/1‑144 (MQ=255)
                                                                                                   gtcTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGcc                                              <  8:239868/149‑1 (MQ=255)
                                                                                                       ggccggccAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTc                                                                                                                                 >  7:45521/1‑62 (MQ=255)
                                                                                                       ggccggccAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTc                                                                                                                                 <  8:45521/62‑1 (MQ=255)
                                                                                                        gccggccAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGAt                                           >  1:310191/1‑147 (MQ=255)
                                                                                                          cggccAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAgg                                       >  7:288861/1‑149 (MQ=255)
                                                                                                             ccAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAGGCAg                                    <  8:288861/149‑1 (MQ=255)
                                                                                                                ggTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAGGCAGccc                                 >  2:29160/1‑149 (MQ=255)
                                                                                                                 gTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAGGCAGCCCt                                >  4:32116/1‑149 (MQ=255)
                                                                                                                   cgcgGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAGGCAGCCCTcg                              <  1:280213‑M2/149‑3 (MQ=255)
                                                                                                                    gcgGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAGGCAGCCCTcg                              >  2:280213‑M2/1‑146 (MQ=255)
                                                                                                                      gggCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAGGCAGCCCTcgcc                            <  8:240695‑M2/148‑5 (MQ=255)
                                                                                                                        gCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGagggctgcctgatcggcgccaacgccggt                         <  4:67313‑M1/149‑30 (MQ=255)
                                                                                                                          cTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGagggctgcctgatcggcgccaacgccggtat                       <  5:257680‑M1/149‑32 (MQ=255)
                                                                                                                          cTTGACCACTTTGACCAGTTCGTAGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAGGCAGCCCTCGCCGACtt                       <  3:187949/149‑1 (MQ=255)
                                                                                                                          cTTGACCACTTTGACCAGTTCGTAGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAGGCAGCCCTCGCCGACtt                       <  3:191865/149‑1 (MQ=255)
                                                                                                                             gACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAGGCAGCCCTCGCCGACTTtga                    >  5:100398/1‑149 (MQ=255)
                                                                                                                              aCCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAGGCAGCCCTCGCCGACTTtgat                   >  5:295046/1‑149 (MQ=255)
                                                                                                                              aCCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAGGCAGCCCTCGCCGACTTtgat                   <  5:103686/149‑1 (MQ=255)
                                                                                                                              aCCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAGGCAGCCCTCGCCGACTTtga                    <  3:127843/148‑1 (MQ=255)
                                                                                                                                cACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGagggctgcctgatcggcgccaacgccggtatcgg                    <  6:72214‑M1/146‑35 (MQ=255)
                                                                                                                                cACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGagggctgcctgatcggcgccaacgccggtatcgg                    >  5:72214‑M1/1‑112 (MQ=255)
                                                                                                                                     tGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCTCCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATAGCGGCGTTGGCGCCGATCAGGCAGCCCTCGCCGACTTTGATGACGAt             <  7:223445/148‑1 (MQ=255)
                                                                                                                                         cAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAGGCAGCCCTCGCCGACTTTGATGACGATGTTgc        >  7:46775/1‑149 (MQ=255)
                                                                                                                                               gttgttCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAGGCAGCCCTCGCCGACTTTGATGACGATGTTGCCGCCAc  <  6:100398/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CAGTTGTGAGCGTGCAGCGCTTCGTTCAGCTCGATGGCCGACTTGTGGGTCTTGCATTCTACCGCGCCGTTCAGCGAGTTGCGGCGGAACAGCAGGTCGGTCTGGCCGGCCAGGTCGCGGGCCTTGACCACTTTGACCAGTTCGTTGTTCTCGTCCAGCAGGTTCACCTTGGTGCCCGCGGTGATGTACAGGCCGGCTTCGACGGTGTTGCGGTCGCCCAACGGAATACCGATACCGGCGTTGGCGCCGATCAGGCAGCCCTCGCCGACTTTGATGACGATGTTGCCGCCAC  >  NC_002947/1736606‑1736897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑