Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,751,428 C→G 11.5% H263D (CAC→GAC)  PP_1551 → replication protein O

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,751,4280CG11.5% 145.5 / 9.6 52H263D (CAC→GAC) PP_1551replication protein O
Reads supporting (aligned to +/- strand):  ref base C (23/23);  new base G (3/3);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

CAGTGCCTGACCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCACGGCGA  >  NC_002947/1751287‑1751574
                                                                                                                                              |                                                                                                                                                  
cAGTGCCTGACCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTGATGACGGCtg                                                                                                                                             >  4:76396/1‑148 (MQ=255)
   tGCCTGACCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTGATGACGGCtgttc                                                                                                                                          <  3:76396/149‑5 (MQ=255)
         aCCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACcg                                                                                                                                    >  3:8339/1‑149 (MQ=255)
           cTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTGATGACGGCtgtt                                                                                                                                           <  8:43443/140‑4 (MQ=255)
           cTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTGATGACGGCtgtt                                                                                                                                           >  7:43443/1‑137 (MQ=255)
                 tATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTaca                                                                                                                            >  3:81649/1‑149 (MQ=255)
                     cAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACAccgc                                                                                                                        >  6:294198/1‑149 (MQ=255)
                       gCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAAc                                                                                                                                         >  4:70827/1‑130 (MQ=255)
                       gCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAAc                                                                                                                                         <  3:70827/130‑1 (MQ=255)
                         cTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGTCTTAATCACGGCTTCAACGACCTCGACTACACCGCCGGc                                                                                                                    <  4:186469/149‑1 (MQ=255)
                            cccAGGTCATGGAAATCGTCATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCTAATATATTTAATCACTGCTTGAACTACGTCTACTACACCGCCGGCCt                                                                                                                  <  4:53110/148‑1 (MQ=255)
                                  tCATGGAAATCGTCATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAg                                                                                                           >  8:278086/1‑149 (MQ=255)
                                  tCATGGAAATCGTCATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAg                                                                                                           >  8:230519/1‑149 (MQ=255)
                                   cATGGAAATCGTCATCGAGAGCGGGTGGGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAg                                                                                                           >  1:166446/1‑149 (MQ=255)
                                          aTCGTGATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCTAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGa                                                                                                   <  3:19092/149‑1 (MQ=255)
                                          aTCGTCATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGa                                                                                                   >  1:185711/1‑149 (MQ=255)
                                               cATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCa                                                                                              >  5:174282/1‑149 (MQ=255)
                                                     gagCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATg                                                                                        >  8:57977/1‑149 (MQ=255)
                                                       gCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCg                                                                                      <  2:185711/149‑1 (MQ=255)
                                                               gcgcAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGTCTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATc                                                                                   <  1:70710/145‑1 (MQ=255)
                                                               gcgcAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATc                                                                                   >  2:70710/1‑145 (MQ=255)
                                                                cgcAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAAt                                                                              >  6:92282/1‑149 (MQ=255)
                                                                cgcAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAAt                                                                              <  5:294198/149‑1 (MQ=255)
                                                                  ctgCTTCGTGGTGGAATGGTTCACCTAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGTTCAGCCGTCATCTCGGCTTCAACGACCGCGACTACAGCGCCGGCCTGTCCGAGCGTGAGGACGGCACGTATGCGATCTGAATc                                                                             <  5:200824/146‑1 (MQ=255)
                                                                     cTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCgg                                                                                                                     <  4:11463/105‑1 (MQ=255)
                                                                     cTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCgg                                                                                                                     >  3:11463/1‑105 (MQ=255)
                                                                     cTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAACCCGAACAGCCGTCATCACGGCTTCAACGACCGCCACTACACCGCCGGCCTCTCCGAGCGAGAGGACGCCACCTATGCGATCTGAATCgag                                                                          >  6:200824/1‑146 (MQ=255)
                                                                           ggTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACt                                                                   <  7:57977/149‑1 (MQ=255)
                                                                               gAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGt                                                               <  7:278086/149‑1 (MQ=255)
                                                                                   ggATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGa                                                                        <  3:166294/136‑1 (MQ=255)
                                                                                   ggATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGa                                                                        >  4:166294/1‑136 (MQ=255)
                                                                                        aCCAAGCGATTCGCCGCCAAATCGCCCGCACAGTGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTg                                                       <  5:136326/148‑1 (MQ=255)
                                                                                        aCCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAg                                                                                                     <  3:210904/102‑1 (MQ=255)
                                                                                        aCCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAg                                                                                                     >  4:210904/1‑102 (MQ=255)
                                                                                           aaGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGa                                                    >  5:263682/1‑148 (MQ=255)
                                                                                           aaGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGa                                                    >  4:187047/1‑148 (MQ=255)
                                                                                              cGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAAcg                                                <  6:174282/149‑1 (MQ=255)
                                                                                                aTTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAAcgcc                                              <  4:8339/149‑1 (MQ=255)
                                                                                                    gccgccAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCCCCTATGCGATCTGAAGCGGTGATTACTATGTCCGAc                                                          >  8:202681/1‑133 (MQ=255)
                                                                                                    gccgccAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGt                                                               >  4:146277/1‑128 (MQ=255)
                                                                                                    gccgccAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGt                                                               <  3:146277/128‑1 (MQ=255)
                                                                                                    gccgccAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACgccgcc                                           <  5:92282/148‑1 (MQ=255)
                                                                                                       gccAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGtt                                       <  7:230519/149‑1 (MQ=255)
                                                                                                                 ccGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCa                             <  2:166446/149‑1 (MQ=255)
                                                                                                                     acaGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCt                         >  1:10291/1‑149 (MQ=255)
                                                                                                                           cAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGccgccg                                          <  7:124743/126‑1 (MQ=255)
                                                                                                                           cAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGccgccg                                          >  8:124743/1‑126 (MQ=255)
                                                                                                                              gCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGc                <  3:187047/149‑1 (MQ=255)
                                                                                                                                 ttgaaGCCGTGATGACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCcgc                                  <  8:157865/124‑1 (MQ=255)
                                                                                                                                 ttgaaGCCGTGATGACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCcgc                                  >  7:157865/5‑128 (MQ=255)
                                                                                                                                 gAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAg             >  3:81217/1‑149 (MQ=255)
                                                                                                                                   aCAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGc            <  2:10291/148‑1 (MQ=255)
                                                                                                                                            atcaCGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCACGGCGa  <  4:81217/149‑1 (MQ=255)
                                                                                                                                             tcaCGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGa              <  7:289881/136‑1 (MQ=255)
                                                                                                                                             tcaCGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGa              >  8:289881/1‑136 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CAGTGCCTGACCTTCGGTATCCAGCCTGCCCAGGTCATGGAAATCGTCATCGAGAGCGGGT‑GGCGCAGCTTCGAGGTGGAATGGATCACCAAGCGATTCGCCGCCAAATCGCCCGCACAGGGCAAGCCGAACAGCCGTCATCACGGCTTCAACGACCGCGACTACACCGCCGGCCTGTCCGAGCGTGAGGACGGCACCTATGCGATCTGAATCGGTGATTACTATGTCCGACGTGCGAAACGCCGCCGGTTTCCGCGTCCAGCCTGCGCACTGCGAGCATCACGGCGA  >  NC_002947/1751287‑1751574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑