Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,765,247	A→C	6.5%	T138P (ACG→CCG)	PP_1574 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,765,247	0	A	C	6.5%	184.3 / 3.6	62	T138P (ACG→CCG)	PP_1574	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (36/22);  new base C (2/2);  total (38/24)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

CCCGGTTGAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGAGCGCGCCACGGGCC  >  NC_002947/1765102‑1765393                                                                                                                                                  |                                                                                                                                                   cccGGTTGAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCAc                                                                                                                                                   >  2:39822/1‑147 (MQ=255)  ccGGTTGAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTAACCGAGCTCGACGAGCTCTGGAAagag                                                                                                                                                >  3:170141/1‑149 (MQ=255)       tGAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGt                                                                                                                                           >  6:311968/1‑149 (MQ=255)         aaCTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACTAGCTCGTc                                                                                                                                          <  5:311968/148‑1 (MQ=255)            tGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCctct                                                                                                                                      >  5:194895/1‑149 (MQ=255)                    gACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATc                                                                                                                              >  3:276662/1‑149 (MQ=255)                       ccGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATcggc                                                                                                                           >  8:58620/1‑149 (MQ=255)                       ccGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATcggc                                                                                                                           <  2:243753/149‑1 (MQ=255)                         ggAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATcggcgg                                                                                                                         >  5:204623/1‑149 (MQ=255)                         ggAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATcggcgg                                                                                                                         <  5:267522/149‑1 (MQ=255)                               cACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTcgac                                                                                                                   >  4:29653/1‑149 (MQ=255)                               cACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTcgac                                                                                                                   <  4:276662/149‑1 (MQ=255)                                       gcCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTcg                                                                                                                     >  3:14909/1‑139 (MQ=255)                                       gcCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTcg                                                                                                                     <  4:14909/139‑1 (MQ=255)                                              aTCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCa                                                                                                     >  3:216185/1‑148 (MQ=255)                                                 tctTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAg                                                                                                  >  4:134234/1‑148 (MQ=255)                                                  ctTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGa                                                                                                 >  1:302059/1‑148 (MQ=255)                                                   ttGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCaaa                                                                                                   <  7:20126/145‑1 (MQ=255)                                                   ttGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCACGACGATCGCGCAGGCCAAAGaa                                                                                                >  1:240797/1‑148 (MQ=255)                                                      aTGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAAc                                                                                             <  3:134234/148‑1 (MQ=255)                                                       tGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCt                                                                                           <  4:216185/149‑1 (MQ=255)                                                        ggCGACCTGTCCGTCGCGCTGCTGGTAGCTATTAGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCt                                                                                           >  5:259104/1‑148 (MQ=255)                                                         gCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGa                                                                                         <  5:324076/149‑1 (MQ=255)                                                              cTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTg                                                                                    <  1:39822/149‑1 (MQ=255)                                                                     tCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGt                                                                             >  7:17630/1‑149 (MQ=255)                                                                             ctgGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTc                                                                      <  8:17630/148‑1 (MQ=255)                                                                                gTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCTGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGa                                                                                         <  6:210962/126‑1 (MQ=255)                                                                                gTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGa                                                                                         >  5:210962/1‑126 (MQ=255)                                                                                gTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTgg                                                                  >  4:93025/1‑149 (MQ=255)                                                                                 tAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTggg                                                                 <  6:204623/149‑1 (MQ=255)                                                                                     tATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGaa                                                             >  8:115940/1‑149 (MQ=255)                                                                                                       ccTGCGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACCAGCTCGTCCTCTCCGGCAACCGCGGCACGACGATCGCGCAGGCCAAAGAAAACCTGAGCCAGAGTGAGTTCAGGTCTTGGGAGAAGTACAGCCAGCTACGTgg                                           >  8:292847/1‑149 (MQ=255)                                                                                                         tGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCt                                         >  3:214215/1‑149 (MQ=255)                                                                                                          gggAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTc                                        >  3:87015/1‑149 (MQ=255)                                                                                                            gAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGc                                      <  7:115940/149‑1 (MQ=255)                                                                                                               aGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGGGAAGTACAGGCAGCTCCGTGGCTCGCTGc                                   <  2:302059/149‑1 (MQ=255)                                                                                                                     agagCTCGTCGAGCTCGGTGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCg                             >  2:144320/1‑149 (MQ=255)                                                                                                                     agagCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAg                                                                         >  5:163005/1‑105 (MQ=255)                                                                                                                     agagCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAg                                                                         <  6:163005/105‑1 (MQ=255)                                                                                                                         cTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGAt                         >  4:145448/1‑149 (MQ=255)                                                                                                                          tCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATg                        >  6:255812/1‑149 (MQ=255)                                                                                                                            aCCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCAt                                 >  5:73551/1‑138 (MQ=255)                                                                                                                            aCCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCAt                                 <  6:73551/138‑1 (MQ=255)                                                                                                                            aCCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATgcg                      >  8:119808/1‑149 (MQ=255)                                                                                                                            aCCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATgcg                      >  6:315372/1‑149 (MQ=255)                                                                                                                            aCCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATgc                       <  7:58620/148‑1 (MQ=255)                                                                                                                              cGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGt                                                                       >  5:201048/1‑98 (MQ=255)                                                                                                                              cGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGt                                                                       <  6:201048/98‑1 (MQ=255)                                                                                                                              cGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGcgcg                    >  6:68055/1‑149 (MQ=255)                                                                                                                                aGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTg                  <  2:240797/149‑1 (MQ=255)                                                                                                                                   tCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCCGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGAg               >  2:270773/1‑149 (MQ=255)                                                                                                                                    cgacgaGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGAgc              >  1:217968/1‑149 (MQ=255)                                                                                                                                     gacgaGCTCGTGCCAGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGt                   <  7:177726/143‑1 (MQ=255)                                                                                                                                     gacgaGCTCGTGCCAGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGt                   <  1:221952/143‑1 (MQ=255)                                                                                                                                     gacgaGCTCGTGCCAGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGt                   >  8:177726/1‑143 (MQ=255)                                                                                                                                     gacgaGCTCGTGCCAGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGt                   >  2:221952/1‑143 (MQ=255)                                                                                                                                             ctGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCg                             <  2:173100/125‑1 (MQ=255)                                                                                                                                             ctGGCACGAGCTCGTCCTCTCCGGCAACGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCg                             >  1:173100/1‑125 (MQ=255)                                                                                                                                              tGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGAGCGCGCCACggg    >  7:40426/1‑149 (MQ=255)                                                                                                                                                gCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGt                   <  7:177311/132‑1 (MQ=255)                                                                                                                                                gCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGt                   >  8:177311/1‑132 (MQ=255)                                                                                                                                                gCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGAGCGCGCCACGGGcc  >  6:278569/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                   CCCGGTTGAACTGGAGAAGGACCCGGAGAGCACCAAGCGCCTGGGATCTCTTGATGGCGACCTGTCCGTCGCGCTGCTGGTAGCTATTCGGGAGGTCAACGACCTGGGAAAGACGAAGAGCTCACCGAGCTCGACGAGCTCTGGCACGAGCTCGTCCTCTCCGGCATCGGCGGCTCGACGATCGCGCAGGCCAAAGAAAACCTGAGCCTGCGTGAGTTCAGGTCTTGGGAGAAGTACAGGCAGCTCCGTGGCTCGCTGCATGTCGGGATGCGCGTGGAGCGCGCCACGGGCC  >  NC_002947/1765102‑1765393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑