Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,795,791 G→A 6.8% Q33Q (CAG→CAA)  lpxD → UDP‑3‑O‑acylglucosamine N‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,795,7910GA 6.8% 174.7 / 2.4 59Q33Q (CAG→CAA) lpxDUDP‑3‑O‑acylglucosamine N‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (26/29);  new base A (2/2);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCACCCGCCAAGTCATCGAGCGCATGAACCAAGCCCGTTGATATGAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGCCGGCAATACCCTGGTCGTGGCGGACCCGT  >  NC_002947/1795649‑1795933
                                                                                                                                               |                                                                                                                                              
cATCACCCGCAAAGTCATCGAGCGCATGAACCAAGCCCGTTGATCTGAGTGTGACCATGACGCTAGTCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCaggagg                                                                                                                                            <  6:169390/147‑1 (MQ=255)
   cACCCGCCAAGTCATCGAGCGCATGAACCAAGCCCGTTGATATGAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGc                                                                                                                                       >  6:36458/1‑149 (MQ=255)
    aCCCGCCAAGTCATCGAGCGCATGAACCAAGCCCGTTGATATGAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGATTTTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGc                                                                                                                                       <  1:253513/149‑1 (MQ=255)
    aCCCGCCAAGTCATCGAGCGCATGAACCAAGCCCGTTGATATGAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGACAGTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGc                                                                                                                                       <  1:257404/149‑1 (MQ=255)
    aCCCGCCAAGTCATCGAGCGCATGAACCAAGCCCGTTGATATGAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGc                                                                                                                                       <  6:208362/148‑1 (MQ=255)
        gCCAAGTCATCGAGCGCATGAACCAAGCCCGTTGATATGAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGAc                                                                                                                                  <  1:115553/149‑1 (MQ=255)
         ccAAGTCATCGAGCGCATGAACCAAGCCCGTTGATATGAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACg                                                                                                                                 >  4:254142/1‑149 (MQ=255)
            aGTCATCGAGCGCATGAACCAAGCCCGTTGATATGAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAg                                                                                                                              <  1:55017/149‑1 (MQ=255)
                        cATGAACCAAGCCCGTTGATATGAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTc                                                                                                                  >  3:131067/1‑149 (MQ=255)
                         aTGAACCAAGCCCGTTGATATGAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCg                                                                                                                 >  1:152426/1‑149 (MQ=255)
                         aTGAACCAAGCCCGTTGATATGAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCg                                                                                                                 >  1:65036/1‑149 (MQ=255)
                                 aGCCCGTTGATATGAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCg                                                                                                         >  8:40396/1‑149 (MQ=255)
                                      gTTGATATGAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTa                                                                                                    >  3:11347/1‑149 (MQ=255)
                                         gATATGAGTGTGACCATGACGCTAGGCCAGCTGGACGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGATGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCg                                                                                                 >  7:175450/1‑149 (MQ=255)
                                            atGAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTAcc                                                                                                  >  8:65929/1‑145 (MQ=255)
                                            atGAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTAcc                                                                                                  <  7:65929/145‑1 (MQ=255)
                                             tGAGTGTGACCATGACGCTAGGCTAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAg                                                                                             >  6:171728/1‑149 (MQ=255)
                                              gAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCagg                                                                                             >  4:234381/1‑146 (MQ=255)
                                                gtgtGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTAc                                                                                          <  3:254142/149‑1 (MQ=255)
                                                   tgACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTg                                                                                       <  7:119658/149‑1 (MQ=255)
                                                     aCCATGACGCTAGGCTAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGa                                                                                     <  5:171728/149‑1 (MQ=255)
                                                     aCCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGa                                                                                     <  3:84769/149‑1 (MQ=255)
                                                     aCCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGa                                                                                     >  4:222326/1‑149 (MQ=255)
                                                      ccATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGAt                                                                                    <  4:11347/149‑1 (MQ=255)
                                                         tGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAAc                                                                                 <  3:1421/149‑1 (MQ=255)
                                                               tAGGCCAGCTGGCCGAGGTACTCGCA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAg                                                                           >  1:3057/1‑149 (MQ=255)
                                                                   gcAGCTGGCGGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTAACTTGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTTCGTGGATAACATCCAGGCCg                                                                       <  8:175450/148‑1 (MQ=255)
                                                                    cAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCgg                                                                      >  1:99210/1‑149 (MQ=255)
                                                                      gCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATaa                                                                                  <  6:143821/135‑1 (MQ=255)
                                                                      gCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATaa                                                                                  >  5:143821/1‑135 (MQ=255)
                                                                           ccGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGtg                                                               <  3:234381/149‑1 (MQ=255)
                                                                           ccGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGtg                                                               <  5:36458/149‑1 (MQ=255)
                                                                            cGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGtgc                                                              >  8:37329/1‑149 (MQ=255)
                                                                                        a‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTg                                                  <  5:120939/149‑1 (MQ=255)
                                                                                          gCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGc                                                 <  7:37329/149‑1 (MQ=255)
                                                                                           cTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCg                                                >  7:989/1‑149 (MQ=255)
                                                                                               ccTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATg                                            >  7:86253/1‑149 (MQ=255)
                                                                                                 tCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGAc                                                                                                                                  >  1:256339/1‑61 (MQ=255)
                                                                                                 tCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGAc                                                                                                                                  <  2:256339/61‑1 (MQ=255)
                                                                                                   aaGGGGCCCAAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCg                                         >  4:43513/1‑148 (MQ=255)
                                                                                                     ggggCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGAt                                             >  7:298123/1‑142 (MQ=255)
                                                                                                     ggggCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGAt                                             <  8:298123/142‑1 (MQ=255)
                                                                                                      gggCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAg                                     <  2:152426/149‑1 (MQ=255)
                                                                                                          ccGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCttt                                 <  3:222326/149‑1 (MQ=255)
                                                                                                           cGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTg                                >  4:135372/1‑149 (MQ=255)
                                                                                                            gaggagCTGCATATTACCGGTTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGc                               <  4:131067/149‑1 (MQ=255)
                                                                                                               gcgcTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGCCgg                            <  2:99210/149‑1 (MQ=255)
                                                                                                                cgcTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGCCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGCCGGc                           <  1:74785/149‑1 (MQ=255)
                                                                                                                    gCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGCCGGCAATa                       >  1:278134/1‑149 (MQ=255)
                                                                                                                          tACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGc                               >  5:323433/1‑135 (MQ=255)
                                                                                                                          tACCGGGTGGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGc                               <  6:323433/135‑1 (MQ=255)
                                                                                                                            ccGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGCCGGCAATACCCTGGTc               >  4:39498/1‑149 (MQ=255)
                                                                                                                              gggTTGGCCACCTTGCAAGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGCCGGCAATACCCTGGTCGt             >  8:222432/1‑149 (MQ=255)
                                                                                                                              gggTTGGACACCTTGCAAGAGGCTGCCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGCCGGCAATACCCTGGTCGt             >  6:253916/1‑149 (MQ=255)
                                                                                                                                     ccACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGCCGGCAATACCCTGGTCGTGGCGGAc      <  8:86253/149‑1 (MQ=255)
                                                                                                                                       aCCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGCCGGCAATACCCTGGTCGTGGCGGAccc    <  3:135372/149‑1 (MQ=255)
                                                                                                                                       aCCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGCCGGCAATACCCTGGTCGTGGCGGAccc    <  3:39498/149‑1 (MQ=255)
                                                                                                                                        ccTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGCCGGCAATACCCTGGTCGTGGCGGACCCg   >  3:304240/1‑149 (MQ=255)
                                                                                                                                         cTTGCAAGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCTGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGCCGGCAATACCCTGGTCGTGGCGGACCCGt  <  5:253916/149‑1 (MQ=255)
                                                                                                                                         cTTGCAAGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGCCGGCAATACCCTGGTCGTGGCGGACCCGt  <  7:222432/149‑1 (MQ=255)
                                                                                                                                              aggaggCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGCCGGCAATACCCTg                  >  7:47765/1‑128 (MQ=255)
                                                                                                                                              aggaggCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGCCGGCAATACCCTg                  <  8:47765/128‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                              
CATCACCCGCCAAGTCATCGAGCGCATGAACCAAGCCCGTTGATATGAGTGTGACCATGACGCTAGGCCAGCTGGCCGAGGTACTCGGA‑GCTACCCTCAAGGGGCCCGAGGCGCTGCAGATTACCGGGTTGGCCACCTTGCAGGAGGCTGGCCCGACGCAGTTGAGCTTCCTCGCCAACCCGCAGTACCGCAAGTACCTGGATAACAGCCAGGCCGGCGCGGTGCTGCTCAAGGCTGCGGATGCCGAAAGCTTTGCCGGCAATACCCTGGTCGTGGCGGACCCGT  >  NC_002947/1795649‑1795933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑