Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,797,345 C→T 12.5% L16L (CTG→TTG)  lpxA → acyl‑[acyl‑carrier‑protein]‑‑UDP‑N‑ acetylglucosamine O‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,797,3450CT12.5% 114.8 / 7.8 48L16L (CTG→TTG) lpxAacyl‑[acyl‑carrier‑protein]‑‑UDP‑N‑ acetylglucosamine O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (24/18);  new base T (2/4);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

TCAGCCGTAAAAGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCAGTTTTCCTCGAT  >  NC_002947/1797203‑1797490
                                                                                                                                              |                                                                                                                                                 
tCAGCCGTAAAAGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGcc                                                                                                                                              >  4:132800/1‑148 (MQ=255)
  aGCCGTAAAAGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGAc                                                                                                                                           <  5:56531/149‑1 (MQ=255)
       tAAAAGCCTGATCTCGAAGCTCGAGTGCCGCGCCCTTGGCGATGCCAAGCCGGTCTGCTCGGCAGAAATGACCTGCGCGGAACGCTCCCTACCAATTCGATTGATCCTCGGGCCATAATCTATCCGTCGGCCAGCTTGGCCGACggatc                                                                                                                                      <  6:305780/149‑4 (MQ=255)
        aaaaGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTg                                                                                                                                                 >  1:10759/1‑137 (MQ=255)
        aaaaGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTg                                                                                                                                                 <  2:10759/137‑1 (MQ=255)
            gCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAgg                                                                                                                                 >  2:171496/1‑149 (MQ=255)
                     ggAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGACCGACGGCGTc                                                                                                                                     <  1:19973/136‑1 (MQ=255)
                     ggAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGACCGACGGCGTc                                                                                                                                     >  2:19973/1‑136 (MQ=255)
                        aGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGt                                                                                                                     <  8:170779/149‑1 (MQ=255)
                                tGCCGCGCCCTTGTCGATGGCACGCCGGGCTGCTCGGCATAAATCACCTGCGCGGAACGCTCCCAATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGtt                                                                                                             <  2:101287/149‑1 (MQ=255)
                                 gCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAGCTTGGCCGAc                                                                                                                                           <  8:79960/118‑1 (MQ=255)
                                 gCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAGCTTGGCCGAc                                                                                                                                           >  7:79960/1‑118 (MQ=255)
                                  ccGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAGCTTGGCCGAc                                                                                                                                           <  1:14847/117‑1 (MQ=255)
                                  ccGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAGCTTGGCCGAc                                                                                                                                           >  2:14847/1‑117 (MQ=255)
                                  ccGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTAGGCCCTTGGTCGATCGTTgg                                                                                                           >  5:202457/1‑149 (MQ=255)
                                     cgcCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGAc                                                                                                                                           <  2:260576/114‑1 (MQ=255)
                                     cgcCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGAc                                                                                                                                           >  1:260576/1‑114 (MQ=255)
                                                     aaGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAGCTTGGCCGAc                                                                                                                                           <  6:179938/98‑1 (MQ=255)
                                                        ccGGTCTGCTCGGCATAAATCACCTGCGCGTAACGCTCCATATGATTTCGATTGATCCTCGGGCAATTATCGATCCGTCGGCCAAGCTGGCCGAGGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGa                                                                                      <  6:202457/148‑1 (MQ=255)
                                                            tCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCa                                                                                 >  1:206537/1‑149 (MQ=255)
                                                                   ggCATAAATCACCTGCGCGTAACGCTCCCTTTGAATTCGATTGATCCTGGGGCCTTTTTCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTa                                                                           <  3:83245/148‑1 (MQ=255)
                                                                           tCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCg                                                                   >  4:205028/1‑148 (MQ=255)
                                                                                tGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCAtgtt                                                             >  4:244902/1‑149 (MQ=255)
                                                                                 gcgcGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACg                                                                                                                                          >  6:240878/1‑71 (MQ=255)
                                                                                 gcgcGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACg                                                                                                                                          <  5:240878/71‑1 (MQ=255)
                                                                                  cgcgGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCAtgttgt                                                           <  3:132800/149‑1 (MQ=255)
                                                                                   gcgGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCAtgttgt                                                           >  3:240347/1‑148 (MQ=255)
                                                                                   gcgGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTtgtg                                                          >  1:68233/1‑149 (MQ=255)
                                                                                     ggAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTTTGc                                                         >  5:184135/1‑148 (MQ=255)
                                                                                                  tGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGAc                                            <  2:206537/148‑1 (MQ=255)
                                                                                                    aaTTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGAACGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCg                                         >  5:212803/1‑149 (MQ=255)
                                                                                                      ttCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCa                                       >  2:199545/1‑149 (MQ=255)
                                                                                                       tCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTg                                                                                                            <  4:193103/79‑1 (MQ=255)
                                                                                                       tCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTg                                                                                                            >  3:193103/1‑79 (MQ=255)
                                                                                                         gATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATAGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCg                                    >  3:185152/1‑149 (MQ=255)
                                                                                                          aTTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCgg                                   >  6:279881/1‑149 (MQ=255)
                                                                                                           ttGATCCTCGGGCCATTATCGATCGGTCGGCCAAGCTGGCGGTCGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGa                                             <  4:251299/138‑1 (MQ=255)
                                                                                                           ttGAACCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATCTTGTGCTCAAAGGCCCGa                                             >  3:251299/1‑138 (MQ=255)
                                                                                                                     ggCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAAcc                        >  5:160707/1‑149 (MQ=255)
                                                                                                                     ggCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAAcc                        <  2:68233/149‑1 (MQ=255)
                                                                                                                               gATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCATGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCa              <  5:279881/149‑1 (MQ=255)
                                                                                                                                aTCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGCAATCGGGGAGGGCCCCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCAg             >  2:184508/1‑149 (MQ=255)
                                                                                                                                aTCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCg                                    >  5:14014/1‑126 (MQ=255)
                                                                                                                                    gTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGAACGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCAGtttt         <  6:212803/149‑1 (MQ=255)
                                                                                                                                       ggCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCAGTTTTCCt      >  3:33728/1‑149 (MQ=255)
                                                                                                                                       ggCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCAGTTTTCCt      >  5:141403/1‑149 (MQ=255)
                                                                                                                                         ccAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCAGTTTTCCTCg    <  3:244902/149‑1 (MQ=255)
                                                                                                                                           aaGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCAGTTTTCCTCGAt  <  3:205028/149‑1 (MQ=255)
                                                                                                                                              cTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCAGTTTTCCTCGa   <  6:243784/145‑1 (MQ=255)
                                                                                                                                              cTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCAGTTTTCCTCGa   >  5:243784/1‑145 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
TCAGCCGTAAAAGCATGATCTGGAAGTTCGAGTGCCGCGCCCTTGTCGATGGCAAGCCGGTCTGCTCGGCAGAAATCACCTGCGCGGAACGCTCCCTATGAATTCGATTGATCCTCGGGCCATTATCGATCCGTCGGCCAAGCTGGCCGACGGCGTCGAGGTCGGCCCTTGGTCGATCGTTGGCCCTGATGTAGAAATCGGGGAGGGCACCGTTATCGGCCCGCATGTTGTGCTCAAAGGCCCGACCCGCATCGGCAAGCACAACCGTATCTTTCAGTTTTCCTCGAT  >  NC_002947/1797203‑1797490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑