| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,836,749 | T→G | 8.1% | V66G (GTT→GGT) | ttcA → | tRNA 2‑thiocytidine(32) synthetase TtcA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,836,749 | 0 | T | G | 8.1% | 180.5 / 3.4 | 59 | V66G (GTT→GGT) | ttcA | tRNA 2‑thiocytidine(32) synthetase TtcA |
| Reads supporting (aligned to +/- strand): ref base T (26/28); new base G (2/3); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGCGAAGCCATCACCGACTACAACATGATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGAAGGATACCTACTCGGTGGTAAAGGAGCTGGTACCCGAAGGCAAGACGACTTGC > NC_002947/1836608‑1836897 | cTGGCGAAGCCATCACCGACTACAACATGATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGt > 8:162383/1‑148 (MQ=255) gCGAAGCCATCACCGACTACAACATGATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAAc < 1:126250/149‑1 (MQ=255) gcaaTCACCGACTACAACATGATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGa < 8:261116/146‑1 (MQ=255) gCCATCACCGACTACAACATGATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATCATCGACGTTCAGTTGAACCTGCAGAAGGTGGCACCGATAACGTTCTAGATCGTTGCGGTGAACATGGa > 7:141064/1‑149 (MQ=255) ccATCACCGACTACAACATGATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGAc < 2:90699/149‑1 (MQ=255) ccATCACCGACTACAACATGATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGAc > 8:278871/1‑149 (MQ=255) ctaCTTCATCATGATCGAGGATGGCGACAAGGTCATGGTCTGTCTGTCCGGCGGCAAGGTCAGCTACACCATGCTCGACGTTGTGTTTCACCTGCAGAAGGTGGCACCGATCACGTTCGTGATCGTTGCGGTGAACATGGACGAGAAAc < 4:316336/147‑1 (MQ=255) acaacaTGATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCg < 6:266721/149‑1 (MQ=255) aacaTGATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACAt > 8:291673/1‑131 (MQ=255) aacaTGATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTCAACAt < 7:291673/131‑1 (MQ=255) acaTGATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCgg > 5:322408/1‑147 (MQ=255) acaTGATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCgg < 6:322408/147‑1 (MQ=255) caTGATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCTCGAACGTGATCGGTg > 7:71302/1‑119 (MQ=255) caTGATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCTCGAACGTGATCGGTg < 8:71302/119‑1 (MQ=255) aTGATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCtt < 7:260604/149‑1 (MQ=255) gATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGTACCAGAAACAGCCGGGCTTcc > 3:121299/1‑149 (MQ=255) cgcggATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCCCCTGCAGAAGGTGGCACCGCTCCCGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCt > 5:27662/4‑148 (MQ=255) gttgATGGCGACCAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCCTGCTCGACGTTCTGTTGCAACTGCAGAAGGGGGCACCGATCACGTTCGAGATCGGGGCGGTGAACAGTGACCCGAAACAGCCGGGCGTCCCTGa < 6:27662/146‑1 (MQ=255) gAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCt > 6:84424/1‑147 (MQ=255) aGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCg < 6:177579/149‑1 (MQ=255) ggTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCgg < 7:162383/149‑1 (MQ=255) gTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAg > 1:271517/1‑114 (MQ=255) gTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAg < 2:271517/114‑1 (MQ=255) tCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATTCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGc > 6:207042/1‑148 (MQ=255) cATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTg > 1:125584/1‑142 (MQ=255) cTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTc > 5:118812/1‑148 (MQ=255) cTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCg > 1:30118/1‑135 (MQ=255) cTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCg < 2:30118/135‑1 (MQ=255) aaGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTggggg > 2:135255/1‑145 (MQ=255) aaGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTggggg < 1:135255/145‑1 (MQ=255) aGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACca > 1:17913/1‑149 (MQ=255) cacCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACAtc < 8:273798/148‑1 (MQ=255) cTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTTGGGGTCGAGTACCACATCGTCGAGaa < 5:207042/149‑1 (MQ=255) cTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCgag > 1:306713/1‑147 (MQ=255) cTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGaa < 6:118812/149‑1 (MQ=255) tCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACAtcgt < 1:172416/142‑1 (MQ=255) tCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACAtcgt > 2:172416/1‑142 (MQ=255) tCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGAAg > 4:289484/1‑149 (MQ=255) cGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGAAg > 6:21074/1‑148 (MQ=255) gTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGAAGGATAc < 5:84424/149‑1 (MQ=255) ttCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGAAGGATAcc < 2:17913/149‑1 (MQ=255) cTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGAAGGATACCTa > 3:50138/1‑149 (MQ=255) ttGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGAAGGATACCTACt < 5:21074/148‑1 (MQ=255) cACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTcc < 3:254468/73‑1 (MQ=255) cACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTcc > 4:254468/1‑73 (MQ=255) aCCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGa < 3:239656/115‑1 (MQ=255) aCCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGa > 4:239656/1‑115 (MQ=255) cAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGAAGGATACCTACTCGGTGGTaaa < 2:306713/149‑1 (MQ=255) aaGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGCTGAACATGGACCAGAAACAGACGGGCTTCCCTGACCACGTACTGCATGCCTACCTCAAGGAGCTGGGCTTCGAGTACCACATCGTCGAGAACCATACCTACTCGGAGGTaa > 4:21070/2‑148 (MQ=255) cgCAACGATCTCGAACGTGATCGGTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGaa < 2:120947/119‑1 (MQ=255) cgCAACGATCTCGAACGTGATCGGTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGaa > 1:120947/2‑120 (MQ=255) gCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGt < 1:270492/103‑1 (MQ=255) gCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGt > 2:270492/1‑103 (MQ=255) gCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGAAGGATACCTACTCGGTGGTAAAGGAGCTgg > 3:283456/1‑148 (MQ=255) aCCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGAAGGATACCTACTCGGTGGTAAAGGAGCTGGTAc < 3:289484/149‑1 (MQ=255) ccGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGAAGGATACCTACTCGGTGGTAAAGGAGCTGGTAcc < 3:21070/149‑1 (MQ=255) aCGGTCGAGATCGTTGCGGTGAACATGCACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCTGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGAAGGATACCTACTCGGTGGTAAAGGAGCTGGTACCCGAAgg < 8:141064/149‑1 (MQ=255) gagaTCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGAAGGATACCTACTCGGTGGTAAAGGAGCTGGTACCCGAAGGCAAgac > 7:94144/1‑149 (MQ=255) gTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCCTCGAGAAGGATACCTACTCGGTGGTAAAGGAGCTGGTACCCGAAGGCAAGACGACTTg < 8:77532/149‑1 (MQ=255) ttGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGAAGGATACCTACTCGGTGGTAAAGGAGCTGGTACCCGAAGGCAAGACGACTTGc < 7:278871/149‑1 (MQ=255) | CTGGCGAAGCCATCACCGACTACAACATGATCGAGGATGGCGACAAGGTCATGGTCTGCCTGTCCGGCGGCAAGGACAGCTACACCATGCTCGACGTTCTGTTGCACCTGCAGAAGGTGGCACCGATCACGTTCGAGATCGTTGCGGTGAACATGGACCAGAAACAGCCGGGCTTCCCTGAGCACGTACTGCCGGCCTACCTCAAGGAGCTGGGGGTCGAGTACCACATCGTCGAGAAGGATACCTACTCGGTGGTAAAGGAGCTGGTACCCGAAGGCAAGACGACTTGC > NC_002947/1836608‑1836897 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |