Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,837,950 G→T 8.9% R190L (CGC→CTC) ‡ PP_1642 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,837,9500GT 8.9% 165.2 / 6.7 56R190L (CGC→CTC) ‡PP_1642hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/26);  new base T (2/3);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

TGGCCAAGCACGGCAACCAGTTGGGCGGGTTGTCGGCGCCACGGTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCATTGAGCCTTGTGTTGCTCCCATTCGCGGGCATGCCCGCTCCCACAG  >  NC_002947/1837806‑1838095
                                                                                                                                                |                                                                                                                                                 
tGGCCAAGCACGGCAACCAGTTGGGCGGGTTGTCGGCGCCACGGTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGAc                                                                                                                                               >  6:40435/1‑149 (MQ=255)
    cAAGCAGGGCAACCAGTTGGGCGGGTTGTCGGCGCCACGGTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACAGATGACATGGCAGCGGCTTTTATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTgg                                                                                                                                           <  7:205157/149‑1 (MQ=255)
       gCACGGCAACCAGTTGGGCGGGTTGTCGGCGCCACGGTTCTTGCGCATGGTCGGCAATGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGAAGCCAACCAGCCAGCGCGACCTGGctc                                                                                                                                        >  7:227208/1‑149 (MQ=255)
       gCACGGCAACCAGTTGGGCGGGTTGTCGGCGCCACGGTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCCACCAGCCAGGTCGCGCTGGctg                                                                                                                                        <  7:49117/149‑2 (MQ=255)
           ggCAACCAGTTGGGCGGGTTGTCGGCGCCACGGTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGt                                                                                                                                    <  5:40435/149‑1 (MQ=255)
            gCAACCAGTTGGGCGGGTTGTCGGCGCCACGGTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGGTCGCGCTGGctggtt                                                                                                                                     >  4:51417/1‑143 (MQ=255)
            gCAACCAGTTGGGCGGGTTGTCGGCGCCACGGTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGGTCGCGCTGGctggtt                                                                                                                                     <  3:51417/147‑5 (MQ=255)
              aaCCAGTTGGGCGGGTTGTCGGCGCCACGGTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTgca                                                                                                                                 <  1:61113/149‑1 (MQ=255)
                   gTTGGGCGGGTTGTCGGCGCCACGGTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGTAGCAgg                                                                                                                            <  7:186393/149‑1 (MQ=255)
                   gTTGGGCGGGTTGTCGGCGCCACGGTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAgg                                                                                                                            >  4:83359/1‑149 (MQ=255)
                        gCGGGTTGTCGGCGCCACGGTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTc                                                                                                                       <  3:83359/149‑1 (MQ=255)
                                tCGGCGCCACGGTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGt                                                                                                                <  7:83237/148‑1 (MQ=255)
                                   gcgcCACGGTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCa                                                                                                            >  2:317821/1‑149 (MQ=255)
                                       cACGGTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAAc                                                                                                                    >  8:175142/1‑137 (MQ=255)
                                       cACGGTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATg                                                                                                          >  6:67587/1‑147 (MQ=255)
                                          ggTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCa                                                                                                        >  6:229408/1‑146 (MQ=255)
                                           gTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCagaga                                                                                                    <  7:34606/149‑1 (MQ=255)
                                               ttGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAgcgg                                                                                                >  5:48141/1‑149 (MQ=255)
                                                              aaGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCa                                                                                 <  5:44755/149‑1 (MQ=255)
                                                                 gACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTg                                                                                                               >  5:218739/1‑116 (MQ=255)
                                                                 gACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTg                                                                                                               <  6:218739/116‑1 (MQ=255)
                                                                  acacGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGTCTCTGGTGCAGCAGGCGTTCAACCAGTGTCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTg                                                                             >  8:286141/1‑149 (MQ=255)
                                                                     cGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCg                                                                          <  3:81495/149‑1 (MQ=255)
                                                                      gTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCgg                                                                         >  3:227993/1‑149 (MQ=255)
                                                                      gTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCgg                                                                         >  6:231228/1‑149 (MQ=255)
                                                                       ttCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGt                                                                        <  1:317821/149‑1 (MQ=255)
                                                                         cATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGa                                                                      >  8:167867/1‑149 (MQ=255)
                                                                          aTCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGa                                                                      <  6:121033/148‑1 (MQ=255)
                                                                            cccGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGc                                                                   >  5:171227/1‑149 (MQ=255)
                                                                              cgaccgaTGACATGGCAGCGGCGTTGATTGCGCAGTAGGTGATCGTCATGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGt                                                                            <  1:126430/138‑1 (MQ=255)
                                                                                    aTGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCGTGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGca                                                            >  6:213683/1‑148 (MQ=255)
                                                                                      gACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGGTCGCGCTGGctggt                                                                                                                                      <  5:229794/72‑4 (MQ=37)
                                                                                      gACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGGTCGCGCTGGctggt                                                                                                                                      >  6:229794/1‑69 (MQ=37)
                                                                                      gACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGTATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCacac                                                         >  1:32289/1‑149 (MQ=255)
                                                                                       aCATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACAcc                                                        <  7:167867/149‑1 (MQ=255)
                                                                                            gCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGTATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCaa                                                   <  2:32289/149‑1 (MQ=255)
                                                                                            gCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCaa                                                   <  5:229408/149‑1 (MQ=255)
                                                                                            gCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCa                                                    <  5:67587/148‑1 (MQ=255)
                                                                                              aGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGAAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAAcc                                                 <  8:227208/149‑1 (MQ=255)
                                                                                                   cgTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCGTGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCATTGa                                            <  5:213683/149‑1 (MQ=255)
                                                                                                       gATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCATTGAGCCt                                        >  5:27268/1‑149 (MQ=255)
                                                                                                            cgcAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCATTGAGCCTTgtgt                                   >  7:153781/1‑149 (MQ=255)
                                                                                                              cAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCATTGAGCCTtgtg                                    <  1:133595/146‑1 (MQ=255)
                                                                                                              cAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCATTGAGCCTtgtg                                    >  2:133595/1‑146 (MQ=255)
                                                                                                              cAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCATTGAGCCTTGTGTTg                                 <  5:169938/149‑1 (MQ=255)
                                                                                                                       aTCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCATTGAGCCTTGTGTTGCTCCCATTc                        <  6:27268/149‑1 (MQ=255)
                                                                                                                           aCAAGCAGCCAACCAGCCAGGGCGACCTGGCTCTGGTGCAGCAGGCGTTCAAGCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCATTGAGCCTTGTGTTGCTCCCATTCGCgg                    <  3:314543/149‑1 (MQ=255)
                                                                                                                           aCAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCAtt                                              <  7:118350/123‑1 (MQ=255)
                                                                                                                           aCAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCAtt                                              >  8:118350/1‑123 (MQ=255)
                                                                                                                           aCAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCATTGAGCCTTGTGTTGCTCCCATTCGCgg                    <  4:227993/149‑1 (MQ=255)
                                                                                                                           aCAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCATTGAGCCTTGTGTTGCTCCCATTCGCgg                    >  3:280032/1‑149 (MQ=255)
                                                                                                                            cAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCATTGAGCCTTGTGTTGCTCCCATTCGCagg                   >  1:162212/1‑149 (MQ=255)
                                                                                                                             aaGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCATTGAGCCTTGTGTTGCTCCCATTCTCGGGc                  <  7:286141/149‑1 (MQ=255)
                                                                                                                                 agcCAACCAGCCAGCGCGACCTGGCTCT‑GTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCATTGAGCCTTGTGTTGCTCCCATTCGCGGGCATGcc             >  3:96602/1‑149 (MQ=255)
                                                                                                                                     aaCCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCATTGAGCCTTGTGTTGCTCCCATTCGCGGGCATGCCCGc          >  3:79093/1‑149 (MQ=255)
                                                                                                                                             agcGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTCGCGCACACCGTCAACCATTGAGCCTTGTGTTGCTCCCATTCGCGGGCATGCCCGCTCCCACAg  >  2:262184/1‑149 (MQ=255)
                                                                                                                                              gcgcGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCacac                                                         >  7:68116/1‑93 (MQ=255)
                                                                                                                                              gcgcGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCacac                                                         <  8:68116/93‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TGGCCAAGCACGGCAACCAGTTGGGCGGGTTGTCGGCGCCACGGTTCTTGCGCATGGTCGGCAAGGACACGTTCATCCCGACCGATGACATGGCAGCGGCGTTGATTGCGCAGAAGGTGATCGACAAGCAGCCAACCAGCCAGCGCGACCTGGCTCTGGTGCAGCAGGCGTTCAACCAGTGGCATGCAGAGAGCGGGCGGCCGCTGTGCCAGTTGTCGGTGATGCTGGCGCACACCGTCAACCATTGAGCCTTGTGTTGCTCCCATTCGCGGGCATGCCCGCTCCCACAG  >  NC_002947/1837806‑1838095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑