Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,843,996	T→G	100%	E266D (GAA→GAC)	gacS ←	sensor protein GacS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,843,996	0	T	G	100.0%	156.8 / NA	47	E266D (GAA→GAC)	gacS	sensor protein GacS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/22);  total (25/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGG  >  NC_002947/1843856‑1844139                                                                                                                                              |                                                                                                                                                ttttGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGt                                                                                                                                         >  5:28220/1‑149 (MQ=255)  tttGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGtt                                                                                                                                        >  1:278598/1‑149 (MQ=255)     gcagcaGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATCTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTc                                                                                                                                      >  8:97222/1‑148 (MQ=255)                   aaGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCa                                                                                                                        <  6:28220/148‑1 (MQ=255)                                   tGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTg                                                                                                        <  8:269575/148‑1 (MQ=255)                                       cagttgccGGATTTCGTGGGTCATGTTGGCCAGGAACTCCGACTTGATGGGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGt                                                                                                      <  7:8852/139‑1 (MQ=255)                                         gggTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATg                                                                                                 <  7:225918/149‑1 (MQ=255)                                               ggATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGAt                                                                                                  <  7:44473/142‑1 (MQ=255)                                               ggATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGAt                                                                                                  >  8:44473/1‑142 (MQ=255)                                                     cGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGt                                                                                     >  4:144057/1‑149 (MQ=255)                                                     cGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCATTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTATCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGt                                                                                     >  5:266111/1‑149 (MQ=255)                                                                    ccAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTc                                                                      >  5:94488/1‑149 (MQ=255)                                                                     cAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGtt                                                                                                                                        <  6:221614/82‑1 (MQ=255)                                                                     cAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGtt                                                                                                                                        >  5:221614/1‑82 (MQ=255)                                                                     cAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTgg                                                                                                                            >  8:143772/1‑94 (MQ=255)                                                                     cAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTgg                                                                                                                            <  7:143772/94‑1 (MQ=255)                                                                         aaCTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCa                                                                  <  7:97222/148‑1 (MQ=255)                                                                            tCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGt                                                              >  5:149949/1‑149 (MQ=255)                                                                                      tGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATg                                                    <  3:144057/149‑1 (MQ=255)                                                                                                cTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGc                                          >  7:84030/1‑149 (MQ=255)                                                                                                 tCAAGTGCCTCTTTGCGCGCCATGTCCAGGCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGc                                          >  1:187394/1‑149 (MQ=255)                                                                                                 tCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGcc                                         >  3:99428/1‑149 (MQ=255)                                                                                                     gTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTgg                                     >  5:26451/1‑149 (MQ=255)                                                                                                        ccTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGc                                   <  6:26451/148‑1 (MQ=255)                                                                                                        ccTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGc                                   <  6:94488/148‑1 (MQ=255)                                                                                                          tctTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGtt                                               >  1:94043/1‑134 (MQ=255)                                                                                                          tctTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGtt                                               <  2:94043/134‑1 (MQ=255)                                                                                                          tctTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGAt                                            >  6:166338/1‑137 (MQ=255)                                                                                                          tctTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGAt                                            <  5:166338/137‑1 (MQ=255)                                                                                                                cgcgcCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTc                          <  8:84030/149‑1 (MQ=255)                                                                                                                 gcgcCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTg                                                                                                                             <  1:233007/49‑1 (MQ=255)                                                                                                                 gcgcCATGTCCA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTg                                                                                                                             >  2:233007/1‑49 (MQ=255)                                                                                                                          ccA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGtt                                               >  3:172285/1‑118 (MQ=255)                                                                                                                          ccA‑GCTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGtt                                               <  4:172285/118‑1 (MQ=255)                                                                                                                               cTCGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTGGTGGGCATTCTGGAGGGTTTCGGCGATGCGGTTGATGCCGTGGGCCAGTTCGTCGTGCTCATGGCTGc            <  6:266111/149‑1 (MQ=255)                                                                                                                                ccGATCTTCTGGATGTCGATTTTTTCCAGGTTCTGTGGCAAGGCTTCTGTGGCGTGGGCGATGCTGTGGTTAAGTTCTTCGTGTGCATTCTGCAGGGTTTCGGCCATGCGGTTTATGCCGTGGGCCAGTTCGTCCAGCTCATtgctgc            <  8:241060/147‑1 (MQ=255)                                                                                                                                 cGATGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTACGTGGGCATTCGGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCACATGGCTTcc           >  8:39136/1‑148 (MQ=255)                                                                                                                                   aTGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCg                            <  7:80544/129‑1 (MQ=255)                                                                                                                                   aTGTTCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGAAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCg                            >  8:80544/1‑129 (MQ=255)                                                                                                                                     ggtgtGGATGGCGATGGTTTCCAGGTTCTGGCGCACGTGTTCGGTGGCCTGGTCGCTGCTGTGTTGCATTTATTCGTGGGCATTCTGCATGGTTTCGGCCATGCGGTTGTTGCCGTGGGCCAGGTCGGCCAGCTCCTGGCTGCCCATg       <  2:187394/144‑1 (MQ=255)                                                                                                                                     gatCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGCCCTGGTCGATGCTGTGTTGCAGTTCTTAGTGGGCATTCTGCAGGGTTTCGGACATGCGGTAGATGCCGTGGGCAAGTTCGTCCAGCTCAATGCTGCCCATgg      >  3:140799/3‑149 (MQ=255)                                                                                                                                       tCTGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTc    >  2:111800/1‑149 (MQ=255)                                                                                                                                         tGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATgg      >  7:62661/1‑145 (MQ=255)                                                                                                                                         tGGATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATgg      <  8:62661/145‑1 (MQ=255)                                                                                                                                           gATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATg                                                    <  3:196648/97‑1 (MQ=255)                                                                                                                                           gATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATg                                                    >  4:196648/1‑97 (MQ=255)                                                                                                                                           gATGTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCgg  >  8:273863/1‑147 (MQ=255)                                                                                                                                              |                                                                                                                                                TTTTGCAGCAGGTGGGTAAAGCCGAGGATGCCATTGAGCGGGGTGCGGATTTCGTGGCTCATGTTGGCCAGGAACTCCGACTTGATGCGGCTGGCCTCAAGTGCCTCTTTGCGCGCCATGTCCA‑GCTCGATGTTCTGGATTTCGATGGTTTCCAGGTTCTGGCGCACGTCTTCGGTGGCCTGGTCGATGCTGTGTTGCAGTTCTTCGTGGGCATTCTGCAGGGTTTCGGCCATGCGGTTGATGCCGTGGGCCAGTTCGTCCAGCTCATGGCTGCCCATGGTCGG  >  NC_002947/1843856‑1844139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑