| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,898,986 | G→A | 10.7% | intergenic (+56/‑464) | PP_t18 → / → PP_1703 | tRNA‑Asp/assimilatory nitrate reductase/sulfite reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,898,986 | 0 | G | A | 10.7% | 140.5 / 7.7 | 57 | intergenic (+56/‑464) | PP_t18/PP_1703 | tRNA‑Asp/assimilatory nitrate reductase/sulfite reductase |
| Reads supporting (aligned to +/- strand): ref base G (27/23); new base A (2/4); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01 | |||||||||||
TCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCGGCATCTCCTGGGACATGGGAGGGCAGTGCTCA > NC_002947/1898842‑1899131 | tCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACCCCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTc > 2:180805/1‑149 (MQ=21)tCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTc > 1:269965/1‑149 (MQ=34)tCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTc > 2:137030/1‑149 (MQ=34) cGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCg > 5:319118/1‑149 (MQ=34) aTGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCt < 1:7350/149‑1 (MQ=34) tataGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCGGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCCTAAGGTCGCtt‑tc < 4:77490/149‑2 (MQ=255) ataGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCtt‑t‑t < 2:269965/148‑1 (MQ=34) taGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCtt‑t‑tt < 6:166796/148‑1 (MQ=34) taGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCCTAAGGTCGCtt‑tct < 1:41255/148‑3 (MQ=19) aGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCtt‑t‑tttt > 7:184689/1‑149 (MQ=34) gcgcAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCtt‑t‑tttt < 3:260384/148‑1 (MQ=34) cGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCt < 1:167153/148‑1 (MQ=39) cGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCt > 2:167153/1‑148 (MQ=39) cAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATg < 6:27411/148‑1 (MQ=255) aaTACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAgg > 5:18127/1‑111 (MQ=33) aaTACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAgg < 6:18127/111‑1 (MQ=33) aaTACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCCTAAGGTCGCtt‑t > 8:34118/1‑119 (MQ=255) aaTACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCCTAAGGTCGCtt‑t < 7:34118/119‑1 (MQ=255) tACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGcc < 2:214197/149‑1 (MQ=255) ccccGGCGTGACAGGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGcc > 1:225923/3‑149 (MQ=255) cGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTTTTTATGCATAATGCAATc > 6:173147/1‑137 (MQ=255) cGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTAt > 3:50473/1‑120 (MQ=38) cGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTAt < 4:50473/120‑1 (MQ=38) gCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGca < 2:91181/125‑1 (MQ=255) gCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGca > 1:91181/1‑125 (MQ=255) gCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATAATCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGc > 8:246884/1‑148 (MQ=255) tGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCtt‑t‑tt > 5:265670/1‑111 (MQ=35) tGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCtt‑t‑tt < 6:265670/111‑1 (MQ=35) aCGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAgg < 6:319118/148‑1 (MQ=255) ggcgggggtcgggggtTCGAGGCCCGTGCGCTGCGCCATATTGCACTTGCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATc < 8:279896/124‑1 (MQ=17) gCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAAAACCCGGAAGTAAAAAAGAATGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCAAGCAATc > 7:279896/1‑126 (MQ=255) cGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGc > 6:283176/1‑147 (MQ=255) aGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAACAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATTAAAAAGTTTACGCCCTGCAACAAGGGGCTATCTGAGCCACtattct > 8:116321/1‑148 (MQ=18) gCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGt > 7:162303/1‑149 (MQ=255) cTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTc < 8:184689/149‑1 (MQ=255) ccATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAATGGGCTAGCTGGGCCACTCTTCTTCCCGCAGCGTCAGCAc < 1:180805/149‑1 (MQ=255) cACTTCCATGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAAc < 8:30908/148‑1 (MQ=255) cACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAAcc > 5:270550/1‑149 (MQ=255) aCTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAAccc < 2:225923/149‑1 (MQ=255) aCTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCGT‑T‑TTTTATTTCTGCATCATGCAATAGTTTAAGCCCGGCAACAAGGGGCTAGCTGCGCCACTCTTGTTCCCGCCGCGTGAGCACCTCCAAccc > 3:163422/1‑149 (MQ=37) aCTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGGCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAAccc < 1:137030/149‑1 (MQ=255) tCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACACTACATCCCGCAGCGTCAGCACCTCAAACCCGTc > 6:275600/1‑149 (MQ=255) aGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTccc > 6:248235/1‑121 (MQ=255) aGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTccc < 5:248235/121‑1 (MQ=255) cACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGTGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCCCCTCAAACCCGTCCTCGATCAc > 5:201379/1‑149 (MQ=255) cACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCAc < 7:116321/149‑1 (MQ=255) cTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTTCT‑TTTTATTTCTGCATCATGCAATAGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCAcc > 1:67257/1‑149 (MQ=255) aaCACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCGGc > 8:236206/1‑148 (MQ=255) cccGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCGGCAtctc < 5:283176/149‑1 (MQ=255) cGGAAGTAAAAAAGAAAGCTACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCGGCATCTCCt < 8:162303/149‑1 (MQ=255) cagaaataaaaaaagCGACCCTAAGGTCGCTT‑TCTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCGGCATCTCCTgg < 5:42417/142‑1 (MQ=38) agaaataaaaaaagCGACCCTAAGGTCGCTT‑TCTTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCGGCATCTCCTgg > 6:42417/7‑148 (MQ=38) aGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCGGCATCTCCTGGGa < 7:236206/149‑1 (MQ=255) tAAAAAAGAAAGCGACCTTATGGTCGCCT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCAc < 6:274790/111‑1 (MQ=255) tAAAAAAGAAAGCGACCTTAGGGTCGCCT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCAc > 5:274790/1‑111 (MQ=255) aaaaaaGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGGTGCGCCACTCTTCTTCCCGCCGCGTCCGCACCTCAAACCCGTGCTCGATCACCGGGCTCTCCGGGGCCAt > 6:106957/1‑149 (MQ=255) aagaaagCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCGGCATCTCCTGGGACATGGGa < 7:246884/149‑1 (MQ=255) gaaagCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTAAGCACCTCACACCCGTCCTCGATCCCCGGCATCTCCGGGGACATTGGAgg > 4:218245/1‑149 (MQ=255) ttAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCGGCATCTCCTGGGACATGGGAGGGCAGTGCTCa > 7:1018/1‑149 (MQ=255) | TCGATGTATAGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTCCACTTCCAGGGCCCACTGAACACCCGGAAGTAAAAAAGAAAGCGACCTTAGGGTCGCTT‑T‑TTTTATTTCTGCATCATGCAATCGTTTACGCCCTGCAACAAGGGGCTAGCTGCGCCACTCTTCTTCCCGCAGCGTCAGCACCTCAAACCCGTCCTCGATCACCGGCATCTCCTGGGACATGGGAGGGCAGTGCTCA > NC_002947/1898842‑1899131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |