Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,944,533 T→G 7.9% intergenic (+34/+228) actP‑I → / ← PP_1744 acetate permease/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,944,5330TG 7.9% 225.0 / 10.5 76intergenic (+34/+228)actP‑I/PP_1744acetate permease/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/36);  new base G (2/4);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.43e-01

GTTCTTCTCGGTCACCGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATC  >  NC_002947/1944385‑1944672
                                                                                                                                                    |                                                                                                                                            
gTTCTTCTCGGTCACCGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGt                                                                                                                                              >  2:288351/1‑149 (MQ=255)
      ctcGGTCACCGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCgg                                                                                                                                           <  3:202468/146‑1 (MQ=255)
           tCACCGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGACAAGCGGGGCGtttt                                                                                                                                   <  7:250519/149‑1 (MQ=255)
            cACCGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGtttt                                                                                                                                   <  4:104156/148‑1 (MQ=255)
            cACCGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGtttt                                                                                                                                   >  3:104156/1‑148 (MQ=255)
             aCCGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGttttt                                                                                                                                  <  1:108806/148‑1 (MQ=255)
               cGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTAt                                                                                                                               >  4:144255/1‑149 (MQ=255)
               cGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGACAAGCGGGGCGttttcaat                                                                                                                               <  6:49907/149‑5 (MQ=255)
                  cAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑Tc                                                                                                                           <  7:288122/149‑1 (MQ=255)
                      tCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCAt                                                                                                                         >  5:212440/1‑147 (MQ=255)
                      tCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCAt                                                                                                                         <  6:212440/147‑1 (MQ=255)
                        cAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAg                                                                                                                     >  3:179865/1‑149 (MQ=255)
                           ggcggcTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTC‑GGGCGTTTTTTATT‑TCATGCAGTCgg                                                                                                                 >  1:20743/1‑149 (MQ=255)
                              ggcTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCg                                                                                                               <  1:294232/149‑1 (MQ=255)
                               gcTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGa                                                                                                              <  3:226664/149‑1 (MQ=255)
                                cTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGAt                                                                                                             >  4:97587/1‑149 (MQ=255)
                                cTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGACAAGCGGGGCGttttcaa                                                                                                                                >  6:186346/1‑128 (MQ=255)
                                cTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGACAAGCGGGGCGttttcaa                                                                                                                                <  5:186346/131‑4 (MQ=255)
                                 tGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACCGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATg                                                                                                            >  3:303174/1‑149 (MQ=255)
                                     gATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCt                                                                                                        >  5:165390/1‑149 (MQ=255)
                                       tGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCTGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTcc                                                                                                      <  1:219667/149‑1 (MQ=255)
                                         aGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCa                                                                                                     <  4:136983/148‑1 (MQ=255)
                                          gcgcgcgcTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGa                                                                                                   <  3:97587/149‑1 (MQ=255)
                                                gcTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGtt                                                                                             >  7:293971/1‑149 (MQ=255)
                                                gcTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGtt                                                                                             >  3:313673/1‑149 (MQ=255)
                                                  tGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTgg                                                                                           >  7:193611/1‑149 (MQ=255)
                                                    ttcttcCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGt                                                                                          <  1:288351/148‑1 (MQ=255)
                                                    ttcttcCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGACAAGCGGGGCGttt                                                                                                                                    <  7:185848/107‑1 (MQ=255)
                                                    ttcttcCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGACAAGCGGGGCGttt                                                                                                                                    >  8:185848/1‑107 (MQ=255)
                                                     tcttcCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTc                                                                                                       <  1:287439/134‑1 (MQ=255)
                                                     tcttcCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTc                                                                                                       >  2:287439/1‑134 (MQ=255)
                                                         cccGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTg                                                                                    >  2:25809/1‑149 (MQ=255)
                                                         cccGCAGTTCGTGCGTTCGCAGACCGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGctct                                                                                     <  4:303174/148‑1 (MQ=255)
                                                          ccGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTg                                                                                         <  6:122214/143‑1 (MQ=255)
                                                          ccGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTg                                                                                         >  5:122214/1‑143 (MQ=255)
                                                             cAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATTCTCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGccc                                                                                 <  1:228546/149‑1 (MQ=255)
                                                             cAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCa                                                                                <  6:121543/149‑1 (MQ=255)
                                                             cAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTC‑GGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCAt                                                                               <  2:20743/149‑1 (MQ=255)
                                                                   gTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATg                                                                              <  4:158002/145‑1 (MQ=255)
                                                                   gTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATg                                                                              >  3:158002/1‑145 (MQ=255)
                                                                      cGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGTTGCTCTGCCCATGCTcccc                                                                        <  4:179865/148‑1 (MQ=255)
                                                                        ttCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCt                                                                            <  8:230243/142‑1 (MQ=255)
                                                                        ttCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCt                                                                            >  7:230243/1‑142 (MQ=255)
                                                                           gCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGt                                                                  >  6:219146/1‑149 (MQ=255)
                                                                              gACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGttt                                                                                                                                    <  8:263455/81‑1 (MQ=255)
                                                                              gACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGttt                                                                                                                                    >  7:263455/1‑81 (MQ=255)
                                                                               aCTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCAt                                                                               <  8:149517/132‑1 (MQ=255)
                                                                               aCTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCAt                                                                               >  7:149517/1‑132 (MQ=255)
                                                                                         gTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGcc                                                                                  >  3:80958/1‑119 (MQ=255)
                                                                                         gTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGcc                                                                                  <  4:80958/119‑1 (MQ=255)
                                                                                         gTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGa                                                             <  3:303027/140‑1 (MQ=255)
                                                                                         gTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGa                                                             >  4:303027/1‑140 (MQ=255)
                                                                                            ccAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATg                                                  <  8:251882/148‑1 (MQ=255)
                                                                                             cAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTgg                                                                                           <  3:182115/106‑1 (MQ=255)
                                                                                             cAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTgg                                                                                           >  4:182115/1‑106 (MQ=255)
                                                                                                            gCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTgg                                 >  2:278741/1‑149 (MQ=255)
                                                                                                                tAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTggg                                <  5:21527/146‑1 (MQ=255)
                                                                                                                tAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTggg                                >  6:21527/1‑146 (MQ=255)
                                                                                                                  aCATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTg                           >  3:236664/1‑149 (MQ=255)
                                                                                                                    aTCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTc                                                                           >  5:97284/1‑99 (MQ=255)
                                                                                                                    aTCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTc                                                                           <  6:97284/99‑1 (MQ=255)
                                                                                                                    aTCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGCCGGCACATTTGGGGGCTGGt                         >  8:84793/1‑149 (MQ=255)
                                                                                                                        aTCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAAt                     >  4:76400/1‑149 (MQ=255)
                                                                                                                        aTCCCCTGCATGGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAAt                     <  8:293971/149‑1 (MQ=255)
                                                                                                                           cccTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTACAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATg                    >  6:185105/1‑147 (MQ=255)
                                                                                                                            ccTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAg                 <  5:219146/149‑1 (MQ=255)
                                                                                                                            ccTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAg                 >  7:86300/1‑149 (MQ=255)
                                                                                                                                cATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCAt             <  6:165390/149‑1 (MQ=255)
                                                                                                                                 ataaaaaaCGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACAttt                                   >  2:76217/5‑126 (MQ=255)
                                                                                                                                 ataaaaaaCGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACAttt                                   <  1:76217/122‑1 (MQ=255)
                                                                                                                                  ttGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCAGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATAc           >  6:241670/1‑149 (MQ=255)
                                                                                                                                    gAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTGTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCaa                      >  3:83606/1‑136 (MQ=255)
                                                                                                                                    gAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACt          <  4:313673/148‑1 (MQ=255)
                                                                                                                                    gAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTg         >  6:150721/1‑149 (MQ=255)
                                                                                                                                     aaaaCGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGt        <  5:185105/149‑1 (MQ=255)
                                                                                                                                     aaaaCGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACAGCACATTTGGGGGCTGGTCAATGAAGCCATACTg         <  3:144255/148‑1 (MQ=255)
                                                                                                                                           ccccGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATc  <  4:236664/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
GTTCTTCTCGGTCACCGACAAGTCCAAGGCGGCTGAGGATGAGCGCGCGCTGTTCTTCCCGCAGTTCGTGCGTTCGCAGACTGGCCTGGGTGCCAGCGGGGCGGTCTCGCACTAACATCAATCCCCTGCATTGAAAACGCCCCGCTTGTCGGGGCGTTTTTTATT‑TCATGCAGTCGGCGATGGCCTCCAGACAGGTTGGTGCTCTGCCCATGCTCCCCTCATGTCGTGAGCGCCTTCATGGAGGACGGCACATTTGGGGGCTGGTCAATGAAGCCATACTGTTTAATC  >  NC_002947/1944385‑1944672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑