Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,956,444 A→G 7.8% I11M (ATA→ATG)  PP_1754 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,956,4440AG 7.8% 142.6 / 2.7 51I11M (ATA→ATG) PP_1754hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/22);  new base G (2/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

TCGAGAACGAGGTCAGCGCGCATACTCATCAGAGGGATGACCTAGCATTTTTGCTAGTTGCGAGTTTTTTGCTATTTGAATCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATT  >  NC_002947/1956298‑1956589
                                                                                                                                                  |                                                                                                                                                 
tCGAGAACGAGGTCAGCGCGCATACTCATCAGAGGGATGACCTAGCATTTTTGCTAGTTGCGAGTTTTTTGCTATTTGAATCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATaaa                                                                                                                                                 <  6:212684/149‑1 (MQ=255)
 cGAGAACGAGGTCAGCGCGCATACTCATCAGAGGGATGACCTAGCATTTTTGCTAGTTGCGAGTTTTTTGATATTTGAATCAAATGGGCATAAAAGATCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATACGGGTATAAAg                                                                                                                                                >  8:50535/1‑149 (MQ=255)
     aaCGAGGTCAGCGCGCATACTCATCAGAGGGATGACCTAGCATTTTTGCTAGTTGCGAGTTTTTTGCTATTTGAATCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTaa                                                                                                                                            <  2:2611/149‑1 (MQ=255)
                       aCTCATCAGAGGGATGACCTAGCATTTTTGCTAGTTGCGAGTTTTTTGCTATTTGAATCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTga                                                                                                                            >  4:262881/1‑147 (MQ=255)
                         tcatcaGAGGGATGACCTAGCATTTTTGCTAGTTGCGAGTTTTTTGCTATTTGAATCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGTTGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAgg                                                                                                                        >  7:93500/1‑149 (MQ=255)
                          catcaGAGGGATGACCTAGCATTTTTGCTAGTTGCGAGTTTTTTGCTATTTGAATCAAATGGGCATAAAAGTACCCCGAGTAAGGGTGAAGAAGGCTCATCCGTTAGTGCATCCGGGTATAAAGGTAAATGTGAATTACATTGAGAGGt                                                                                                                       >  4:45063/1‑149 (MQ=255)
                              agagGGATGACCTAGCATTTTTGCTAGTTGCGAGTTTTTTGCTATTTGATTCAAATGCGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCAGCCGGGTATAAAGGAAAATGTGACTTACCTTGAGAGGTTCgg                                                                                                                   >  2:268720/1‑148 (MQ=255)
                                atggATGACCTAGCATTTTTGCTAGTTGCGAGTTTTTTGCTATTTGAATCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGc                                                                                                                 <  6:176789/147‑1 (MQ=255)
                                 gggCTGACCTAGCCTTTATGCTAGTTGCGAGGTTTTTGCTATTTGAATCAAATGGGCAGAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCg                                                                                                                <  8:132821/149‑1 (MQ=255)
                                         cTAGCATTTTTGCTAGTTGCGAGTTTTTTGCTATTTGAATCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAAt                                                                                                                                    <  7:243623/121‑1 (MQ=255)
                                         cTAGCATTTTTGCTAGTTGCGAGTTTTTTGCTATTTGAATCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAAt                                                                                                                                    >  8:243623/1‑121 (MQ=255)
                                              aTTTTTGCTAGTTGCGAGTTTTTTGCTATTTGAATCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGt                                                                                                                   >  8:246872/1‑133 (MQ=255)
                                               tttttGCTAGTTGCGAGTTTTTTGCTATTTGAATCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATc                                                                                                  <  3:272317/149‑1 (MQ=255)
                                                       aGTTGCGAGTTTTTTGCTATTTGAATCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCt                                                                                          >  2:148676/1‑149 (MQ=255)
                                                            cGAGTTTTTTGCTATTTGAATCAAATGGGCATAATAGTTCGCGTAGTAAGGGTGATGAATGCTCATGCGTTTGTGCATCGGGGTATAAAGGTAAATGTGAATTAGCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGaa                                                                                     <  7:50535/149‑1 (MQ=255)
                                                                          tttGAATCAAATGGGGATTTAAGTTCGCGGAGTAAGTGTGATGAAGGCTCATGCGTTTGTGCATCCGTGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTg                                                                          <  3:45063/146‑1 (MQ=255)
                                                                             gAATCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGt                                                                                                                   >  7:117214/1‑102 (MQ=255)
                                                                             gAATCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGt                                                                                                                   <  8:112791/102‑1 (MQ=255)
                                                                             gAATCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGt                                                                                                                   <  8:117214/102‑1 (MQ=255)
                                                                             gAATCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGt                                                                                                                   >  7:112791/1‑102 (MQ=255)
                                                                              aaTCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTAGTGCGTCATAATTTGCATCGTGAAACTTGGAAGAGTGCCATTGAAAAAg                                                                    >  5:57364/1‑148 (MQ=255)
                                                                                  aaaTGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACt                                                                <  8:93500/148‑1 (MQ=255)
                                                                                    aTGGGCATTAAAGTTAGCGGAGTAAGGGTGATGAATGGTCATGCGTTTGTGCATCCGGGTATAACGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATATTTTGGATCGTGAAGCTTGGAAGAGTGGCAGTGAAAATGTACGGaa                                                             <  6:57364/149‑1 (MQ=255)
                                                                                           tAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTAt                                                      >  5:316692/1‑149 (MQ=255)
                                                                                             aaaGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCTGGTATAAAGGTAAATGTGAATTACCTTGAGATGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTAtt                                                     <  3:262881/148‑1 (MQ=255)
                                                                                             aaaGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTAttt                                                    >  5:280486/1‑149 (MQ=255)
                                                                                              aaGTTCGCGGAGTAAGGGTGATGATGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTCAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTg                                                   <  8:282947/149‑1 (MQ=255)
                                                                                                gTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGa                                                  >  4:265979/1‑148 (MQ=255)
                                                                                                   cgcgGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGTGTATAAAGGTAAATGTGACTTACCTTGAGAGGTTCGTGCGTCATAATTTACATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGaaaa                                               <  8:114998/148‑1 (MQ=255)
                                                                                                             gggTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATaaa                                    <  5:213853/149‑1 (MQ=255)
                                                                                                               gTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGa                                  >  7:161870/1‑149 (MQ=255)
                                                                                                                tgatgaAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGAc                                 >  1:146562/1‑149 (MQ=255)
                                                                                                                  atgaAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTg                               >  2:161530/1‑149 (MQ=255)
                                                                                                                   tgaAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTgg                              <  6:280486/149‑1 (MQ=255)
                                                                                                                   tgaAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTgg                              >  6:39067/1‑149 (MQ=255)
                                                                                                                        gCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGTCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTcc                         <  3:158061/149‑1 (MQ=255)
                                                                                                                          tCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCg                        >  8:49099/1‑148 (MQ=255)
                                                                                                                            aTGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGaa                      <  8:161870/148‑1 (MQ=255)
                                                                                                                                gTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGAt                                       >  5:39003/1‑127 (MQ=255)
                                                                                                                                gTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGAt                                       <  6:39003/127‑1 (MQ=255)
                                                                                                                                 tttGTGCATCCGGGTATGAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAaga                                             <  6:72971/120‑1 (MQ=255)
                                                                                                                                 tttGTGCATCCGGGTATGAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAaga                                             >  5:68315/1‑120 (MQ=255)
                                                                                                                                 tttGTGCATCCGGGTATGAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAaga                                             >  5:72971/1‑120 (MQ=255)
                                                                                                                                 tttGTGCATCCGGGTATGAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAaga                                             <  6:68315/120‑1 (MQ=255)
                                                                                                                                   tgtgCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCcgcgc               <  1:148676/148‑1 (MQ=255)
                                                                                                                                        aTCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTg           >  7:59156/1‑147 (MQ=255)
                                                                                                                                        aTCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTg           >  2:116512/1‑147 (MQ=255)
                                                                                                                                           cGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGTCTTCCGAAGCCGCGCGCTGTAGt       <  7:49099/148‑1 (MQ=255)
                                                                                                                                           cGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGaa                      >  4:74023/1‑133 (MQ=255)
                                                                                                                                           cGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGaa                      <  3:74023/133‑1 (MQ=255)
                                                                                                                                            gggTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTg      >  4:197785/1‑148 (MQ=255)
                                                                                                                                             ggTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGa    >  8:141200/1‑149 (MQ=255)
                                                                                                                                               tataAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGAtt  <  7:141200/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TCGAGAACGAGGTCAGCGCGCATACTCATCAGAGGGATGACCTAGCATTTTTGCTAGTTGCGAGTTTTTTGCTATTTGAATCAAATGGGCATAAAAGTTCGCGGAGTAAGGGTGATGAAGGCTCATGCGTTTGTGCATCCGGGTATAAAGGTAAATGTGAATTACCTTGAGAGGTTCGTGCGTCATAATTTGCATCGTGAACCTTGGAAGAGTGCCAGTGAAAAAGTACTGAATGACTATTTGAAAAGAGCAGATAAAGACTGGGTTCCGAAGCCGCGCGCTGTAGTGGATT  >  NC_002947/1956298‑1956589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑