Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,016,815 A→T 8.5% intergenic (‑423/‑1911) PP_1795 ← / → PP_1797 hypothetical protein/HlyD family secretion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,016,8150AT 8.5% 136.4 / 2.1 47intergenic (‑423/‑1911)PP_1795/PP_1797hypothetical protein/HlyD family secretion protein
Reads supporting (aligned to +/- strand):  ref base A (23/20);  new base T (2/2);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GTTTGAGCGCAATGTGTTTGCATGGTGCGGTTTTTTTGACATGGCTTCAATAGCTTTTCCTGCGTGGATAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTACCTCAGTATGGATACCATGAGTTCACAATCCGAAAATAACCTG  >  NC_002947/2016667‑2016948
                                                                                                                                                    |                                                                                                                                     
gTTTGAGCGCAATGTGTTTGCATGGTGCGGTTTTTTTGACATGGCTTCAATAGCTTTTCCTGCGTGGATAGCCGTTTGGCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCa                                                                                                                                       <  5:324503/149‑1 (MQ=255)
 tttGAGCGCAATGTGTTTGCATGGTGCGGTTTTTTTGACATGGCTTCAATAGCTTTTCCTGCGTGGATAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAg                                                                                                                                      >  3:186002/1‑149 (MQ=255)
       ctcAATGTGTTTGAATGGTGCGGTTTTTTTGACATGGCTTCAATAGCTTTTCCTGCGTGGATATCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGAtt                                                                                                                                <  8:281216/147‑1 (MQ=255)
        gcAATGTGTTTGCATGGTGCGGTTTTTTTGACATGGCTTCAATAGCTTTTCCTGCGTGGATAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGAtt                                                                                                                                >  2:163928/1‑148 (MQ=255)
         cAATGTGTTTGCATGGTGCGGTTTTTTTGACATGGCTTCAATAGCTTTTCCTGCGTGGATAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGa                                                                                                                              <  7:45484/149‑1 (MQ=255)
                tttGCATGGTGCGGTTTTTTTGACATGGCTTCAATAGCTTTTCCTGCGTGGATAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACgg                                                                                                                        >  4:150763/1‑148 (MQ=255)
                   gCATGGTGCGGTTTTTTTGACATGGCTTCAATAGCTTTTCCTGCGTGGATAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAAc                                                                                                                     >  6:144638/1‑148 (MQ=255)
                      tGGTGCGGTTTTTTTGACATGGCTTCAATAGCTTTTCCTGCGTGGATAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGt                                                                                                                 <  4:186002/149‑1 (MQ=255)
                       ggTGCGGTTTTTTTGACATGGCTTCAATAGCTTTTCCTGCGTGGATAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGt                                                                                                                 >  8:2238/1‑148 (MQ=255)
                              tttttttGACATGGCTTCAATAGCTTTTCCTGCGTGGATAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTcc                                                                                                         <  5:144638/149‑1 (MQ=255)
                              tttttttGACATGGCTTCAATAGCTTTTCCTGCGTGGATAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTcc                                                                                                         <  7:60240/149‑1 (MQ=255)
                                        aTGGCTTCAATAGCTTTTCCTGCGTGGATAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTg                                                                                               <  7:2238/149‑1 (MQ=255)
                                                     cTTTTCCTGCGTGGATAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCTGGCGATTGATAACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTc                                                                                  >  2:11169/1‑149 (MQ=255)
                                                     cTTTTCCTGCGTGGATAGCCGTTTGTCGTATCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTATGGAGATTGATCACTGAACGAGTCAGATTCCTGACAATCTGACACGCAGCTGTc                                                                                  >  2:16288/1‑149 (MQ=255)
                                                            tGCGTGGATAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGt                                                                                   <  4:275673/141‑1 (MQ=255)
                                                            tGCGTGGATAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGt                                                                                   >  3:275673/1‑141 (MQ=255)
                                                                tGGATAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGAt                                                                       <  3:150763/149‑1 (MQ=255)
                                                                   aTAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGtt                                                                    <  3:259247/149‑1 (MQ=255)
                                                                       ccGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCa                                                                                        <  2:185022/125‑1 (MQ=255)
                                                                       ccGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCa                                                                                        >  1:185022/1‑125 (MQ=255)
                                                                       ccGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTacac                                                                >  6:19179/1‑149 (MQ=255)
                                                                          tttGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGc                                                             <  1:163928/149‑1 (MQ=255)
                                                                               cgtcTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTaca                                                                 >  4:96221/1‑140 (MQ=255)
                                                                               cgtcTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTaca                                                                 <  3:96221/140‑1 (MQ=255)
                                                                                   tcGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAAt                                                                                                  <  4:1374/103‑1 (MQ=255)
                                                                                   tcGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAAt                                                                                                  >  3:1374/1‑103 (MQ=255)
                                                                                         tCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACaa                                                                                                   >  4:285188/1‑96 (MQ=255)
                                                                                         tCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACaa                                                                                                   <  3:285188/96‑1 (MQ=255)
                                                                                           aCGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTacac                                                                <  5:275029/129‑1 (MQ=255)
                                                                                           aCGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTacac                                                                >  6:275029/1‑129 (MQ=255)
                                                                                           aCGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTAc                                            <  6:217502/149‑1 (MQ=255)
                                                                                           aCGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTAc                                            >  5:217502/1‑149 (MQ=255)
                                                                                           aCGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTAc                                            >  2:19467/1‑149 (MQ=255)
                                                                                             gCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTACCt                                          >  7:165788/1‑149 (MQ=255)
                                                                                                         tGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTACCTCAGTATGGATAc                              >  6:253169/1‑149 (MQ=255)
                                                                                                                  tCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTACCTCAGTATGGATACCATGAGtt                      >  6:117887/1‑148 (MQ=255)
                                                                                                                    aGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTACCTCAGTATGGATACCATGAGTTca                    >  5:41481/1‑148 (MQ=255)
                                                                                                                     gCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTACCTCAGTa                                     >  7:138345/1‑130 (MQ=255)
                                                                                                                     gCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTACCTCAGTa                                     <  8:138345/130‑1 (MQ=255)
                                                                                                                     gCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTACCTCAGTATGGATACCATGAGTTcac                   >  7:122525/1‑148 (MQ=255)
                                                                                                                        aGATTTCTCGCCAAAAATTTTATGATTCTGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTACCTCAGTATGGATACCATGAGTTCACAATc               <  1:11169/149‑1 (MQ=255)
                                                                                                                        aGATTTCTCGCAAAAAATTTTATGATTCTGGCTATTGATAACGTAACGAGTCAGATTCCGGACAATCTGACAATCAGCTGTCAAGATGAGGATGTTACACTTCCCGCCGCTTTCTCCTACATCAGTATGGATACCATGAGTTCACAATc               <  1:16288/149‑1 (MQ=255)
                                                                                                                              ctcGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTACCTCAGTATGGATACCATGAGTTCACAATCCGaaaa         >  6:42869/1‑149 (MQ=255)
                                                                                                                                  ccAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTACCTCAGTATGGATACCATGAGTTCACAATCCGAAAATAAc     <  8:122525/149‑1 (MQ=255)
                                                                                                                                   cAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTACCTCAGTATGGATACCATGAGTTCACAATCCGAAAATAAcc    >  3:232237/1‑149 (MQ=255)
                                                                                                                                     aaaaTTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTACCTCAGTATGGATACCATGAGTTCACAATCCGAAAATAACCTg  <  6:41481/149‑1 (MQ=255)
                                                                                                                                                aTTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTACCTCAGTATGGATACCATGAGTTca                    >  6:300884/1‑120 (MQ=255)
                                                                                                                                                aTTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTACCTCAGTATGGATACCATGAGTTca                    <  5:300884/120‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                     
GTTTGAGCGCAATGTGTTTGCATGGTGCGGTTTTTTTGACATGGCTTCAATAGCTTTTCCTGCGTGGATAGCCGTTTGTCGTCTCGTGCTCACGCATGCAGAGGCTGGAAAATATCAGCAAGATTTCTCGCCAAAAATTTTATGATTCAGGCGATTGATCACGGAACGAGTCAGCTTCCGGACAATCTGACACGCAGCTGTCCCGATGCGGATGTTACACTGCCCGCCGCTTTCGCCTACCTCAGTATGGATACCATGAGTTCACAATCCGAAAATAACCTG  >  NC_002947/2016667‑2016948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑