| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,019,894 | T→C | 6.9% | V390A (GTC→GCC) | PP_1797 → | HlyD family secretion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,019,894 | 0 | T | C | 6.9% | 172.0 / 2.0 | 58 | V390A (GTC→GCC) | PP_1797 | HlyD family secretion protein |
| Reads supporting (aligned to +/- strand): ref base T (28/26); new base C (2/2); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
ACCGATTGGAGCTGGGCAAGACTGCGGACGTCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTACCTGTTGAAACCAGCGCGCAACATGTTCGCTGAATCGCT > NC_002947/2019747‑2020038 | gacGATTGGAGCTGGGCAAGACTGCGGACGTCCGCTTCTCTTCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGCGCGCTCGGGGGACGAGTACTACCTGGTCCGCGt > 2:71317/3‑148 (MQ=255)aCCGATTGGAGCTGGGCAAGACTGCGGACGTCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGt > 8:257589/1‑148 (MQ=255) cGATTGGAGCTGGGCAAGACTGCGGACGTCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTc > 5:16450/1‑147 (MQ=255) gATTGGAGCTGGGCAAGACTGCGGACGTCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAg < 2:244215/149‑1 (MQ=255) aTTGGAGCTGGGCAAGACTGCGGACGTCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAgg < 8:37763/149‑1 (MQ=255) aTTGGAGCTGGGCAAGACTGCGGACGTCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGCCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAgg > 3:293360/1‑149 (MQ=255) tGGAGCTGGGCAAGACTGCGGACGTCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGt < 7:257589/148‑1 (MQ=255) gAGCTGGGCAAGACTGCGGACGTCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCa < 1:149798/148‑1 (MQ=255) aGCTGGGCAAGACTGCGGACGTCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACt < 5:296261/149‑1 (MQ=255) aGCTGGGCAAGACTGCGGACGTCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACt < 3:33852/149‑1 (MQ=255) ggCAAGACTGCGGACGTCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAg < 6:156968/148‑1 (MQ=255) ggCAAGACTGCGGACGTCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGCCAAGGTCACTGAAGa > 7:81311/1‑149 (MQ=255) gACTGCGGACGTCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCa < 7:122186/149‑1 (MQ=255) aCTGCGGACGTCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCAt > 3:294401/1‑149 (MQ=255) cTGCGGACGTCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCAt < 6:16450/148‑1 (MQ=255) tCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGAAGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACtt > 6:289040/1‑149 (MQ=255) cGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGCCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTg < 4:293360/148‑1 (MQ=255) tGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCg < 6:292176/147‑1 (MQ=255) tGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCg > 5:292176/1‑147 (MQ=255) tGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGc > 7:246544/1‑148 (MQ=255) cAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCtt < 8:246544/148‑1 (MQ=255) aCCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGCCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCaa > 5:30519/1‑149 (MQ=255) gCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCgg < 4:294401/149‑1 (MQ=255) accacGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAg < 7:84853/96‑1 (MQ=255) accacGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAg > 8:84853/1‑96 (MQ=255) cacGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGc > 8:8443/1‑149 (MQ=255) gCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTg < 8:167580/119‑1 (MQ=255) gCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTg > 7:167580/1‑119 (MQ=255) cGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGCCGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCt > 6:72643/1‑149 (MQ=255) tCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCaa < 1:210667/121‑1 (MQ=255) tCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCaa > 2:210667/1‑121 (MQ=255) tCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGc > 1:113593/1‑138 (MQ=255) tCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGc < 2:113593/138‑1 (MQ=255) gAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGAAGGCATGAAACAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGCAggact > 8:76932/1‑145 (MQ=255) ggTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCtca > 5:302764/1‑148 (MQ=255) tAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCtcatc > 5:275701/1‑148 (MQ=255) tGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACg > 3:269435/1‑147 (MQ=255) aCGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAg < 4:269435/148‑1 (MQ=255) ggATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGAc < 6:275701/149‑1 (MQ=255) cGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAg > 5:111836/1‑138 (MQ=255) cGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAg < 6:111836/138‑1 (MQ=255) cGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCAc > 2:162338/1‑148 (MQ=255) gCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCAt > 6:233014/1‑149 (MQ=255) cAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCAtgctg > 7:236319/1‑148 (MQ=255) gTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGCCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTAc < 8:81311/147‑1 (MQ=255) aaGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTACCTGTTg < 6:302764/149‑1 (MQ=255) agagCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCa < 5:315738/92‑1 (MQ=255) agagCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCa > 6:315738/1‑92 (MQ=255) cTCGGGGGACCAGTACTACCTGGTCCGCGCCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTACCTGTTGAAACCAg < 6:30519/149‑1 (MQ=255) tCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGc < 6:242366/110‑1 (MQ=255) cAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTACCTGTTGAAACCAGCGCGCAACAt > 1:99157/1‑149 (MQ=255) tacCTGGTCCGCGTCAAGGTCACTGAAGACGGAATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTACCTGTTGAAACCAGCGCGCAACATGTTCGc > 3:121226/1‑149 (MQ=255) ccTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTACCTGTTGAAACCAGCGCGCAACATGTTCGCt > 8:679/1‑148 (MQ=255) tCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTACCTGTTGAAACCAGCGCGCAACATGTTCGCTGAATCg > 1:89773/1‑149 (MQ=255) tCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACACGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTACCTGTTGAAACCAGCGCGCAACATGTTCGCTGAATCg > 8:160234/1‑149 (MQ=255) ccGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTACCTGTTGAAACCAGCGCGCAACAt > 1:175823/1‑135 (MQ=255) ccGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTACCTGTTGAAACCAGCGCGCAACAt < 2:175823/135‑1 (MQ=255) cgcgTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTACCTGTTGAAACCAGCGCGCAACATGTTCGCTGAATCGc < 7:8443/148‑1 (MQ=255) cgcgTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTACCTGTTGAAACCAGCGCGCAACATGTTCGCTGAATCGc < 2:99157/148‑1 (MQ=255) gcgTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTACCTGTTGAAACCAGCGCGCAACATGTTCGCTGAATCGCt > 2:230344/1‑148 (MQ=255) tCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTACCTGTTGAAACCAGCGCGCAAc > 1:41290/1‑128 (MQ=255) tCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTACCTGTTGAAACCAGCGCGCAAc < 2:41290/128‑1 (MQ=255) | ACCGATTGGAGCTGGGCAAGACTGCGGACGTCCGCTTCTCTGCGTTCAACCAGGCCACCACGCCGGTCATCGAAGGTAAGTTGACGCGGATCTCGGCCGACAGCCTGGTCGAAGAGCGCTCGGGGGACCAGTACTACCTGGTCCGCGTCAAGGTCACTGAAGACGGCATGAAAAAACTTGGCAACCGCAAGCTTCAACCCGGCATGCCAGCTGAAGTCCTCATCAACGCAGGCGACCGCACCATGCTGCAGTACCTGTTGAAACCAGCGCGCAACATGTTCGCTGAATCGCT > NC_002947/2019747‑2020038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |