Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,062,851 G→A 7.3% C589Y (TGC→TAC) ‡ PP_1838 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,062,8510GA 7.3% 195.3 / 4.5 68C589Y (TGC→TAC) ‡PP_1838membrane protein
Reads supporting (aligned to +/- strand):  ref base G (34/29);  new base A (2/3);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01

CTGGGCCGCTTCAACGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGACATGAGCCCGCGGATG  >  NC_002947/2062709‑2062990
                                                                                                                                              |                                                                                                                                           
cTGGGCCGCTTCAACGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTgggg                                                                                                                                       >  4:34204/1‑149 (MQ=255)
    gCCGCTTCAACGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGgcgc                                                                                                                                   <  3:34204/149‑1 (MQ=255)
     ccGCTTCAACGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCCCCAGCACTGGTGgcgag                                                                                                                                  <  3:96402/149‑3 (MQ=255)
       gCTTCAACGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGAc                                                                                                                                <  6:309925/149‑1 (MQ=255)
        cTTCAACGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGAcc                                                                                                                               <  7:62882/149‑1 (MQ=255)
         ttCAACGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCt                                                                                                                              >  7:179467/1‑149 (MQ=255)
              cGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCGCGCCCCCAGCACTGGTGgcgagcctt                                                                                                                              >  3:195003/1‑138 (MQ=255)
              cGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCGCGCCCCCAGCACTGGTGgcgagcctt                                                                                                                              <  4:195003/144‑7 (MQ=255)
                   cGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTg                                                                                                                    >  6:298032/1‑149 (MQ=255)
                        ctgctgATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGTATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGc                                                                                                                            >  3:257054/1‑136 (MQ=255)
                        ctgctgATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGTATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGc                                                                                                                            <  4:257054/136‑1 (MQ=255)
                        ctgctgATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCCCCAGCACTggt                                                                                                                                        >  2:277664/1‑123 (MQ=255)
                        ctgctgATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCCCCAGCACTggt                                                                                                                                        <  1:277664/124‑2 (MQ=255)
                        ctgctgATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAAcc                                                                                                                      <  4:252505/142‑1 (MQ=255)
                        ctgctgATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAAcc                                                                                                                      >  3:252505/1‑142 (MQ=255)
                           ctgATTGCCCCGGGCATTCAGGAGAATTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGCGTGGCAAGGCTCGCCACCAGTGCTCGGGGCGCGACCTGCTCAACCTGCCCGAggg                                                                                                            >  1:168347/1‑149 (MQ=255)
                             gATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGt                                                                                                           >  6:242194/1‑148 (MQ=255)
                             gATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTg                                                                                                          >  7:235078/1‑149 (MQ=255)
                              aTTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCTGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCTAGGCTGGCCACCAGTGCGGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTg                                                                                                          <  5:267448/148‑1 (MQ=255)
                                tGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGAc                                                                                                        >  1:75072/1‑148 (MQ=255)
                                    ccGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAgg                                                                                                    <  2:286455/148‑1 (MQ=255)
                                    ccGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAgg                                                                                                    >  1:286455/1‑148 (MQ=255)
                                     cGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGc                                                                                                  >  5:235176/1‑149 (MQ=255)
                                         cATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAg                                                                                              <  8:235078/149‑1 (MQ=255)
                                         cATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAg                                                                                              <  6:235176/149‑1 (MQ=255)
                                            tCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAggg                                                                                                   >  5:145834/1‑141 (MQ=255)
                                            tCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAggg                                                                                                   <  6:145834/141‑1 (MQ=255)
                                             cAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCa                                                                                           <  5:298032/148‑1 (MQ=255)
                                              atgagaATTTGGGTGGGGTGAATCAAACCGTGGGTAACCATGTCGTCATAGTGCCGACGATCATTGGCCGCGTGGGTGGCAATTCTCGCTACCATTGCTGGGGTCGAGACCTGCTCAACCTGCCCGAGGGTTACCAGGGCTTAGGCAt                                                                                          <  2:294756/146‑1 (MQ=255)
                                              aGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCAt                                                                                          >  4:165072/1‑148 (MQ=255)
                                              aGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCCACATCGTGCCGACAATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCAt                                                                                          >  6:116885/1‑148 (MQ=255)
                                               ggAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGa                                                                                        >  4:278486/1‑149 (MQ=255)
                                                gagaAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGAt                                                                                       >  2:93550/1‑149 (MQ=255)
                                                   aaGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCaa                                                                                    <  1:93550/149‑1 (MQ=255)
                                                     gTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAg                                                                                   <  7:273092/148‑1 (MQ=255)
                                                     gTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAg                                                                                   >  8:273092/1‑148 (MQ=255)
                                                      ttCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGcc                                                                                 <  1:39531/149‑1 (MQ=255)
                                                            gcggtgAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCggg                                                                           >  3:101685/1‑149 (MQ=255)
                                                             cggtgAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGc                                                                          <  8:179467/149‑1 (MQ=255)
                                                                      acacCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAg                                                                    >  7:30577/1‑146 (MQ=255)
                                                                       cacCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAg                                                                    >  3:222513/1‑145 (MQ=255)
                                                                         ccGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGAtt                                                              >  2:116158/1‑149 (MQ=255)
                                                                              ggTACCCAGGTCGACATCGTGCCAACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCACGCCGTCGGGCAGCGAGCAGATTGTCgg                                                         >  7:166114/1‑149 (MQ=255)
                                                                                 aCCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCgggag                                                      >  1:275431/1‑147 (MQ=255)
                                                                                 aCCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCgg                                                         <  2:275431/146‑1 (MQ=255)
                                                                                  cccAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTg                                                     <  3:165072/149‑1 (MQ=255)
                                                                                   ccAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTgg                                                    >  3:40928/1‑149 (MQ=255)
                                                                                     aGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTg                                                  <  2:75072/149‑1 (MQ=255)
                                                                                          gACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGccc                                                                                                                 <  2:195740/81‑1 (MQ=255)
                                                                                          gACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGccc                                                                                                                 >  1:195740/1‑81 (MQ=255)
                                                                                          gACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAgg                                              <  8:30577/148‑1 (MQ=255)
                                                                                                 tGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGt                                      <  4:40928/149‑1 (MQ=255)
                                                                                                           catGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATc                             >  2:120900/1‑148 (MQ=255)
                                                                                                                gccgccTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTcc                       <  4:101685/149‑1 (MQ=255)
                                                                                                                 ccgccTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTcc                       >  5:42760/1‑148 (MQ=255)
                                                                                                                  cgccTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCaa                     <  3:61350/149‑1 (MQ=255)
                                                                                                                  cgccTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCa                      >  4:61350/1‑148 (MQ=255)
                                                                                                                    ccTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAg                    >  7:32393/1‑148 (MQ=255)
                                                                                                                        ggTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGGTGGTGCAGGGTGACCGTATCCTGATCGATTCCAAGGACAt               <  5:116885/149‑1 (MQ=255)
                                                                                                                        ggTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGACAt               >  2:215035/1‑149 (MQ=255)
                                                                                                                          tGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGACATGa             <  4:222513/149‑1 (MQ=255)
                                                                                                                                ggCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGACATGAGCCcgc       <  3:278486/149‑1 (MQ=255)
                                                                                                                                 gCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCTACCTGCCCGTGGGTGACCAGGGCGTTGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTGCAAGGACATGAGCCcgc       <  2:168347/148‑1 (MQ=255)
                                                                                                                                 gCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATc                                   <  4:174312/120‑1 (MQ=255)
                                                                                                                                 gCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATc                                   >  3:174312/1‑120 (MQ=255)
                                                                                                                                  cTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGACATGAGCCcgcg      >  8:23418/1‑148 (MQ=255)
                                                                                                                                     gCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGACATGAGCCCGCGGATg  >  2:250011/1‑149 (MQ=255)
                                                                                                                                      ccaccaGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGGGACCGTATCCTGATCGAGTCCAAGGACATGAGCCCGCGGATg  >  6:187228/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                           
CTGGGCCGCTTCAACGTGCCGCTGCTGCTGATTGCCCCGGGCATTCAGGAGAAGTTCGGTGCGGTGAATCACACCGTGGGTACCCAGGTCGACATCGTGCCGACCATCATGGGCCGCCTGGGTGGCAAGGCTCGCCACCAGTGCTGGGGGCGCGACCTGCTCAACCTGCCCGAGGGTGACCAGGGCGTAGGCATGATCAAGCCGTCGGGCAGCGAGCAGATTGTCGGGCTGGTGCAGGGTGACCGTATCCTGATCGAGTCCAAGGACATGAGCCCGCGGATG  >  NC_002947/2062709‑2062990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑