| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,082,281 | T→A | 8.8% | *318L (TAA→TTA) | PP_1861 ← | LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,082,281 | 0 | T | A | 8.8% | 161.8 / 5.6 | 57 | *318L (TAA→TTA) | PP_1861 | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (28/24); new base A (2/3); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01 | |||||||||||
TCAGCAACGAGGTGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCTGGTCTCAGCTTCAGG > NC_002947/2082137‑2082426 | tcaGCAACGAGGTGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATc < 7:4992/149‑1 (MQ=255) aGCACCGAGGGGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGATTAATTcgc < 4:177332/149‑1 (MQ=255) gCAACGAGGTGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATcgcg > 6:210519/1‑149 (MQ=255) gCAACGAGGTGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATcgcg < 8:221703/149‑1 (MQ=255) aaCGAGGTGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGATTAATTCGCGCa < 7:32473/149‑1 (MQ=255) ggTGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTc > 5:57397/1‑148 (MQ=255) ggTGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCa < 5:210519/149‑1 (MQ=255) gTGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCAc < 5:33696/149‑1 (MQ=255) tGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCATTGCGCGATTAATTCGCGCactga > 2:58901/1‑142 (MQ=255) tGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCATTGCGCGATTAATTCGCGCactga < 1:58901/146‑5 (MQ=255) cAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCaa < 4:169178/148‑1 (MQ=255) gggCGAAACAGACCTGGTTGTCAAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGc > 2:106649/1‑149 (MQ=255) ggCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAgga > 4:18212/1‑147 (MQ=255) ggCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGc > 8:49060/1‑148 (MQ=255) gCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGc > 1:97387/1‑147 (MQ=255) cGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATACACTGCGCAAGGCCAg > 2:228966/1‑149 (MQ=255) aaCAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTc > 3:191814/1‑149 (MQ=255) aCCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCAAgg > 6:89923/1‑149 (MQ=255) cTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGt < 5:89923/149‑1 (MQ=255) cTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGAACGCTGATCATAAGTGCGCGAATTAATCGCGCAATTAAATGAGCAAGGCCAGGTCCCAGCGt > 7:19675/1‑149 (MQ=255) tGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTg > 5:252458/1‑149 (MQ=255) gTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAg < 1:88016/136‑1 (MQ=255) gTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAg > 2:88016/1‑136 (MQ=255) ccAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCgg > 8:73520/1‑149 (MQ=255) cagcagcagCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCggg < 1:106649/149‑1 (MQ=255) cagcagcagCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCggg < 6:57397/149‑1 (MQ=255) agcTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGc < 6:138152/149‑1 (MQ=255) tCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGctct > 2:319157/1‑149 (MQ=255) aggaggCTTGTGTGTCGGCGTTCATGCAGGTCCTTGAAACTCATAAATGGAAAATTCTGCGACGCTGATCATCAGTTCGCGAATTAATCGCGCAATTCACTGCGCAATGCCAGGTCCCAGGGTGTAATCGGGCTTAAGCGGCTCTTGat < 2:80946/149‑2 (MQ=255) aggaggCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAg > 8:215323/1‑149 (MQ=255) ttGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTc > 8:18719/1‑148 (MQ=255) gtgtCGGCGTTCATGCAGCTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATAGCGCAATTCACTGCGCAAGGACAGGTCCCAGGGTGGAATCGGGCTGAAGCGCCACTTCAGGAATTCGagc > 2:72941/1‑148 (MQ=255) gTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACgg > 7:299240/1‑149 (MQ=255) cATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGa < 7:87927/126‑1 (MQ=255) cATGCAGCTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGa > 8:87927/1‑126 (MQ=255) aGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACAGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCga > 3:304312/1‑147 (MQ=255) aGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCAt < 1:319157/149‑1 (MQ=255) atcaATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTg > 7:156637/1‑105 (MQ=255) atcaATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTg < 8:156637/105‑1 (MQ=255) atcaATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGCCTCCGAGAGTTCGTTTCATGTGc > 5:190660/1‑149 (MQ=255) tGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGc > 2:182623/1‑149 (MQ=255) aaaaTTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCa > 5:271469/1‑143 (MQ=255) aaaaTTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCa < 6:271469/143‑1 (MQ=255) aaaaTTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCg > 3:293813/1‑147 (MQ=255) aaaTTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAg < 7:18719/149‑1 (MQ=255) ttCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCAt < 3:250150/133‑1 (MQ=255) ttCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCAt > 4:250150/1‑133 (MQ=255) gCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGt < 7:73520/146‑1 (MQ=255) cACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAAt < 4:191814/149‑1 (MQ=255) cACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAAt > 4:109228/1‑149 (MQ=255) gATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCt > 5:254122/1‑149 (MQ=255) atcatcAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCTg < 1:182623/149‑1 (MQ=255) aattGCGCGATTAATTCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCTGGtctc > 3:290264/4‑149 (MQ=255) tGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCCGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAATCGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCTGGTCTCAGc < 2:97387/149‑1 (MQ=255) cgcgAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCTGGTCTCAGCtt < 4:293813/149‑1 (MQ=255) cgATTTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCGAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCTGGTCTCAGCTTCa < 1:72941/149‑1 (MQ=255) aaTTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCTGGTCTCAGCTTCAgg < 7:215323/149‑1 (MQ=255) | TCAGCAACGAGGTGGAGTGCAGGGCGAAACAGACCTGGTTGTCCAGCAGCAGCTCGTCACAGGAGGCTTGGGTGTCGGCGTTCATGCAGGTCCTTGAAAGTCATCAATGGAAAATTCTGGCACGCTGATCATCAGTGCGCGAATTAATCGCGCAATTCACTGCGCAAGGCCAGGTCCCAGGGTGGAATCGGGCTGAAGCGGCTCTTGAGGAATTCGAGCAGCAAACGGCTGCGAGAGTTCGTTTCATGTGCCAGGCGTAGCGCGTAAATGCCGCTGGTCTCAGCTTCAGG > NC_002947/2082137‑2082426 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |