| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 196,795 | G→C | 10.1% | T767T (ACG→ACC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 196,795 | 0 | G | C | 10.1% | 123.6 / 5.5 | 49 | T767T (ACG→ACC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (20/24); new base C (3/2); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01 | |||||||||||
TTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCT > NC_002947/196659‑196935 | ttGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGAc > 5:297932/1‑148 (MQ=37) aGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGa < 7:110402/149‑1 (MQ=37) gCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGAt < 4:184337/149‑1 (MQ=37) gCAATACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACTACATCGGGTCGACCGTCAGCGTCACCATCGAGAGCGCCACTGGCGGC‑AACTTGGAACATCTGACGCGGAAGCCGACGCCGGCTCAGACCACGATCAAc < 7:186551/149‑1 (MQ=11) gCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAAc < 1:238666/149‑1 (MQ=37) gCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAAc > 2:171637/1‑149 (MQ=37) gCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGGTCGACCGTCAGCGTCACCATCGAGAGCGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGAc > 3:114056/1‑139 (MQ=18) gCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGATCGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGAc < 4:114056/139‑1 (MQ=18) aCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTc < 8:82154/149‑1 (MQ=34) aGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGc < 7:152276/149‑1 (MQ=34) tCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGccac > 1:166619/1‑148 (MQ=34) aTTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCaccacc > 4:246679/1‑149 (MQ=34) tttCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACcg > 3:28448/1‑149 (MQ=34) tCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACcgc < 3:246679/148‑1 (MQ=34) ccAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGa > 5:155076/1‑149 (MQ=34) aTGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGc < 2:166619/149‑1 (MQ=32) tGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCCCCGCGACCCTGACGGCGAGcc > 6:250455/1‑149 (MQ=17) aCGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGa < 3:73807/132‑1 (MQ=32) aCGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGa > 4:73807/1‑132 (MQ=32) caacaaCGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGAcc < 3:93935/127‑1 (MQ=32) caacaaCGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGAcc > 4:93935/1‑127 (MQ=32) aacaacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCAc > 8:84503/1‑149 (MQ=32) caacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGt < 1:64209/140‑1 (MQ=32) caacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGt > 2:64209/1‑140 (MQ=32) caacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCAcc > 7:178836/1‑148 (MQ=32) gACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGgcggcg > 3:65182/1‑149 (MQ=32) gTCAGCGTCACCATCGAGAACGCCACTGGCGGCAAACTTCGAACAGCTGCCCCCGAACCCGCCGCCGGCTCAGCCCACGATCAACGACTCGGTCGATGCACCCACCGCGACCCTGCCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGa > 1:209906/1‑149 (MQ=11) gTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGAt < 5:250455/149‑1 (MQ=32) tCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATc < 7:84503/149‑1 (MQ=32) tCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGCCTCGGTCGATGCCACCACCGCGACCCTGACGGCGCGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACAccagc > 5:164573/1‑146 (MQ=12) tCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTacac < 6:164573/145‑1 (MQ=32) gagaACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGa < 6:297932/148‑1 (MQ=32) cGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGa < 4:28448/149‑1 (MQ=34) gcggc‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGACCACCTACACCGTGACCCTGAGCAATc < 1:171637/147‑1 (MQ=17) c‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGCCTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGcc > 4:70764/1‑148 (MQ=17) cAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGt < 6:155076/149‑1 (MQ=32) aCCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAg < 4:128336/148‑1 (MQ=32) aCCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGt < 4:11518/149‑1 (MQ=32) aCCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGt > 6:24672/1‑149 (MQ=17) ccccGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGt < 8:178836/148‑1 (MQ=32) cccGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGa > 2:39413/1‑149 (MQ=32) ccGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAc < 5:197919/149‑1 (MQ=17) ccGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAc < 7:248991/149‑1 (MQ=17) ccGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAc > 1:182108/1‑149 (MQ=17) cGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAcc < 4:65182/149‑1 (MQ=32) cGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGAGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAcc > 6:51033/1‑149 (MQ=12) gAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAcca > 7:172773/1‑148 (MQ=17) gAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAc < 8:172773/147‑1 (MQ=32) aaCCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCt > 5:258773/1‑149 (MQ=32) | TTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGC‑AACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCT > NC_002947/196659‑196935 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |