| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,085,482 | G→C | 8.2% | intergenic (+191/‑404) | PP_1864 → / → PP_1865 | hypothetical protein/transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,085,482 | 0 | G | C | 8.2% | 129.4 / 4.7 | 49 | intergenic (+191/‑404) | PP_1864/PP_1865 | hypothetical protein/transposase |
| Reads supporting (aligned to +/- strand): ref base G (25/20); new base C (2/2); total (27/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01 | |||||||||||
CTGCTTCTGCTTCTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGT > NC_002947/2085337‑2085625 | ctgcttctgcttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGAt < 3:195769/148‑1 (MQ=255)ctgcttctgcttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATg > 1:97599/1‑149 (MQ=255) tgcttctgcttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATgg > 3:294754/1‑149 (MQ=255) cttctgcttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATggg < 2:201008/148‑1 (MQ=40) cttctgcttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATg < 7:283758/146‑1 (MQ=40) cttctgctCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTg > 4:273023/1‑147 (MQ=35) tctTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGt > 3:138075/1‑149 (MQ=32) tctTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGAAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGt > 5:232024/1‑149 (MQ=17) cTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGtcctcc > 2:232604/1‑149 (MQ=32) ggCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCgg < 7:231374/148‑1 (MQ=32) cTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAg < 1:232604/149‑1 (MQ=32) ttttGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCgg > 7:212247/1‑145 (MQ=32) ttttGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCgg < 8:212247/145‑1 (MQ=32) gCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGtcctc > 2:142645/1‑137 (MQ=32) gCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGtcctc < 1:142645/137‑1 (MQ=32) ttCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGTGAGCAAGccc > 5:326277/1‑149 (MQ=17) cTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGGGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGccc < 3:134259/147‑1 (MQ=17) tAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCg > 2:22411/1‑147 (MQ=32) aaGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAg > 5:23006/1‑149 (MQ=32) gcgcgGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAg > 6:331665/1‑149 (MQ=32) gcgcgGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAg < 4:294754/149‑1 (MQ=32) cgcgGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAgg > 5:170279/1‑149 (MQ=32) ggCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGcc > 4:49284/1‑99 (MQ=33) ggCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGcc < 3:49284/99‑1 (MQ=33) gCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGagcg > 6:304227/1‑149 (MQ=32) gATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGagcgta < 4:138075/149‑1 (MQ=32) gaggCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAggg > 7:132754/1‑149 (MQ=32) ccGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGc > 3:264845/1‑149 (MQ=32) cGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGcc > 5:317844/1‑149 (MQ=32) gAGGGCTGGTCTTGCGGAGGTTGTGGACGGGCTTGTAGGCCGGTAATGCGCTGGGCCCCAGGATGGGGCCTGCAGCGGGGTCCTCCCGGTAGCAAGCCCGGAGTTAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCg < 4:264845/149‑1 (MQ=11) aGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCt > 3:214741/1‑149 (MQ=33) gCTTGCGGAGGGAGGTGCCGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTg < 3:119852/149‑1 (MQ=21) gCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTg < 3:273023/149‑1 (MQ=34) gCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTg < 2:97599/149‑1 (MQ=34) gCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGACCaaa < 5:304227/149‑1 (MQ=34) gCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGACCaaa < 4:214741/149‑1 (MQ=34) ggATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGACCAAAGTaa > 8:195518/1‑149 (MQ=34) ccGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGa < 5:264360/134‑1 (MQ=34) ccGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGa > 6:264360/1‑134 (MQ=34) gTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGACCAAAGTAACccgccg > 8:82701/1‑148 (MQ=34) cgcTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCGGGTGTGCGGGGACCCCGGAGCGTAGCGTAGGGGCGGTATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGc < 6:232024/149‑1 (MQ=11) tGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCACGCAGAGTAGCGTAGCGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCAGGAAGGGCgg > 5:224510/1‑148 (MQ=11) caggCCCCATCCTGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCtt > 4:149926/3‑105 (MQ=11) caggCCCCATCCTGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCtt < 3:149926/103‑1 (MQ=11) caggCCCCATCCTGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGaa > 6:163332/3‑142 (MQ=255) caggCCCCATCCTGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGaa < 5:163332/140‑1 (MQ=255) gCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAg > 2:64294/1‑149 (MQ=34) cccAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGt < 6:317844/149‑1 (MQ=34) ccAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGt > 5:105193/1‑148 (MQ=34) | CTGCTTCTGCTTCTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGATGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGGGCCCCAGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGAGGGGAGCCGACGGCTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGT > NC_002947/2085337‑2085625 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |