Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,118,723 C→G 10.4% P800R (CCG→CGG)  PP_1884 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,118,7230CG10.4% 136.1 / 7.5 48P800R (CCG→CGG) PP_1884hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (21/22);  new base G (2/3);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.56e-01

GCAGATCGATATCGATGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAATTACCTCTC  >  NC_002947/2118580‑2118869
                                                                                                                                               |                                                                                                                                                  
gCAGATCGATATCGATGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTgg                                                                                                                                               >  3:251426/1‑149 (MQ=255)
 cAGATCGATATCGATGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTggg                                                                                                                                              <  1:274961/149‑1 (MQ=255)
    atcgatatcgatGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGgca                                                                                                                                           <  3:22931/149‑1 (MQ=255)
        atatcgatGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCtgccgctgccgc                                                                                                                                                  <  7:273903/138‑1 (MQ=255)
        atatcgatGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCtgccgctgccgc                                                                                                                                                  >  8:273903/1‑138 (MQ=255)
        atatcgatGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTgggg                                                                                                                                             >  6:39314/1‑143 (MQ=255)
        atatcgatGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTgggg                                                                                                                                             <  5:39314/143‑1 (MQ=255)
          atcgatGAAGAGGGCTTGCCTTAATAGCATGCCGAGGCCGCTAGCCCCGCTCATTTGTTCGTTGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATAGTGCTGCCGCTGCCGCTGGGGCAGcaca                                                                                                                                      <  3:311137/148‑1 (MQ=255)
           tcgatGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCCCAGCGGCAGCGGCAGcacgat                                                                                                                                    <  6:107270/149‑4 (MQ=255)
                 aaGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGc                                                                                                                               <  3:128372/148‑1 (MQ=255)
                   gACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCt                                                                                                                             >  8:98835/1‑148 (MQ=255)
                   gACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCt                                                                                                                             <  7:98835/148‑1 (MQ=255)
                   gACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCCCAGCGGCAGCGGCAGcac                                                                                                                                       >  4:333/1‑138 (MQ=255)
                   gACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCCCAGCGGCAGCGGCAGcac                                                                                                                                       <  3:333/138‑1 (MQ=255)
                    aCGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTAt                                                                                                                           >  1:174603/1‑149 (MQ=255)
                      ggCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTgggg                                                                                                                                             <  7:187623/129‑1 (MQ=255)
                      ggCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTgggg                                                                                                                                             >  8:187623/1‑129 (MQ=255)
                      ggCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTAt                                                                                                                           >  5:71799/1‑147 (MQ=255)
                        cAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAgg                                                                                                                       <  2:174603/149‑1 (MQ=255)
                          aGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGcc                                                                                                                     <  2:20620/149‑1 (MQ=255)
                           gCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGccc                                                                                                                    <  4:251426/149‑1 (MQ=255)
                              ttACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCtt                                                                                                                  >  4:128444/1‑148 (MQ=255)
                                  tacCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAg                                                                                                               >  2:177513/1‑147 (MQ=255)
                                    ccAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTTAgaagg                                                                                                           >  3:57084/1‑149 (MQ=255)
                                    ccAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAgc                                                                                                              >  1:47472/1‑146 (MQ=255)
                                    ccAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAgc                                                                                                              <  2:47472/146‑1 (MQ=255)
                                    ccAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCCAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCAATCAGGCCCTTGAccagc                                                                                                           >  6:13540/1‑148 (MQ=255)
                                    ccAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGAGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGACAAGCCTATCAGGCCCTTGAgcagg                                                                                                           >  3:52762/1‑149 (MQ=255)
                                            aCGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGTaa                                                                                                   <  3:128444/149‑1 (MQ=255)
                                                cgcgAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGTCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACggg                                                                                               <  1:70531/149‑1 (MQ=255)
                                                          gCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAg                                                                                     <  6:71799/149‑1 (MQ=255)
                                                             caTTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTa                                                                                  <  7:233816/149‑1 (MQ=255)
                                                                   tGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAAc                                                                            >  7:64678/1‑149 (MQ=255)
                                                                                            tctcCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCg                                                                       >  7:217613/1‑129 (MQ=255)
                                                                                            tctcCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCg                                                                       <  8:217613/129‑1 (MQ=255)
                                                                                            tctcCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCtt                                                   >  4:242524/1‑149 (MQ=255)
                                                                                             ctcCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCtt                                                   >  4:108521/1‑148 (MQ=255)
                                                                                                ccccGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCTACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTc                                                 <  5:13540/147‑1 (MQ=255)
                                                                                                  ccGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCa                                             >  6:105095/1‑149 (MQ=255)
                                                                                                       ttACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCa                                        >  7:27044/1‑149 (MQ=255)
                                                                                                          ccccTCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGtt                                     <  4:57084/149‑1 (MQ=255)
                                                                                                          cccATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGtt                                     <  4:52762/149‑1 (MQ=255)
                                                                                                          cccATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGt                                      <  3:242524/148‑1 (MQ=255)
                                                                                                             aTCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAg                                  >  5:149315/1‑149 (MQ=255)
                                                                                                             aTCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAg                                  <  5:105095/149‑1 (MQ=255)
                                                                                                                  gCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCgggg                             <  3:108521/149‑1 (MQ=255)
                                                                                                                              cATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGcc                 >  6:313082/1‑149 (MQ=255)
                                                                                                                               aTCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGcc                 >  2:283348/1‑148 (MQ=255)
                                                                                                                                  gTGCTGCCCCAGCGGCAGCGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCTTACCTTTCa                                                >  7:112255/1‑114 (MQ=255)
                                                                                                                                  gTGCTGCCCCAGCGGCAGCGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCa                                                <  8:112255/114‑1 (MQ=255)
                                                                                                                                        ccgctgccgctgGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAATTAc       <  1:177513/149‑1 (MQ=255)
                                                                                                                                              ccgctgGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAATTACCtctc  <  5:313082/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GCAGATCGATATCGATGAAGACGGCAAGCCTTACTACCAGGCCGACGCCGCGAGCCCCGCACATTTCTGCGTGGTTGGTGCGGTCTGGAGGCTCTCCCCCGACTTACCCATCACGCTCGAGGGTTTCATCGTGCTGCCGCTGCCGCTGGGGCAGCACAGTCAAGCCTATCAGGCCCTTGAGCAGGCAGCGCAACGGGCAACGCGCAGGTAGTCAACCGGCGTTCACCTAAACCCATACCTTTCAGCACTCCAGTTGAGCGGGGCCACATCATGCCTACCAATTACCTCTC  >  NC_002947/2118580‑2118869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑