Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,133,333 T→A 9.7% N91I (AAT→ATT)  fimI ← type I pili subunit FimI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,133,3330TA 9.7% 165.1 / 4.5 59N91I (AAT→ATT) fimItype I pili subunit FimI
Reads supporting (aligned to +/- strand):  ref base T (30/23);  new base A (3/3);  total (33/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAATCAACCGAGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGTCACCGGGATTGAC  >  NC_002947/2133186‑2133481
                                                                                                                                                   |                                                                                                                                                    
tAATCAACCGAGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCAtt                                                                                                                                                     <  4:240379/149‑1 (MQ=255)
      aCCGAGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTc                                                                                                                                               >  5:240461/1‑149 (MQ=255)
         gAGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGACAATGCCTTCgtg                                                                                                                                            <  8:11460/149‑3 (MQ=255)
             ttCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGAt                                                                                                                                         >  5:165465/1‑148 (MQ=255)
                cACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGAtgctg                                                                                                                                     <  6:240461/149‑1 (MQ=255)
                 aCCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCAGGCACTGTTACCAATGCTGAAATAGTCGCAGCTGGCATCAGCGGCACCGACAAACTTCACGAAGGCAATGTATTCGCTGAagctg                                                                                                                                     >  7:293508/1‑148 (MQ=255)
                  ccGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCa                                                                                                                                   <  6:165465/149‑1 (MQ=255)
                  ccGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCa                                                                                                                                   <  2:84396/149‑1 (MQ=255)
                   cGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGAt                                                                                                                                         >  1:251421/1‑142 (MQ=255)
                   cGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGAt                                                                                                                                         <  2:251421/142‑1 (MQ=255)
                     ggTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGAc                                                                                                                                >  4:161919/1‑149 (MQ=255)
                          gATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGttt                                                                                                                           >  8:206574/1‑149 (MQ=255)
                           aTCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGtttt                                                                                                                          >  7:119983/1‑149 (MQ=255)
                            tCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTg                                                                                                                         >  7:256750/1‑149 (MQ=255)
                              gATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTc                                                                                                                       <  8:119983/149‑1 (MQ=255)
                              gATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCAGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCAACGTCAAACTTAACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTc                                                                                                                       >  5:12791/1‑149 (MQ=255)
                                  ccTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCggg                                                                                                                    >  2:6425/1‑148 (MQ=255)
                                   cTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTa                                                                                                                  >  1:46665/1‑149 (MQ=255)
                                          ttGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGTACCGTCAAACTTCACGAAGACAATGCCTTCg                                                                                                                                              <  3:233516/114‑1 (MQ=255)
                                          ttGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGTACCGTCAAACTTCACGAAGACAATGCCTTCg                                                                                                                                              >  4:233516/1‑114 (MQ=255)
                                            gtgccTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTCCCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATg                                                                                                          <  8:277627/145‑1 (MQ=255)
                                            gTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGACAATGCCTTCgtga                                                                                                                                           <  2:289567/115‑4 (MQ=255)
                                            gTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGACAATGCCTTCgtga                                                                                                                                           >  1:289567/1‑112 (MQ=255)
                                                  gATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCCGAGGCACCGTCAAACTTCACGAAGGCATTGTCTTAGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTaa                                                                                                      >  4:261472/1‑146 (MQ=255)
                                                          tCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCa                                                                                           >  5:99866/1‑149 (MQ=255)
                                                             agaTGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACg                                                                                         >  2:278416/1‑148 (MQ=255)
                                                              gaTGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGa                                                                                       >  4:260320/1‑149 (MQ=255)
                                                                  ttttCTGCCGTGACTGAACTGTTACCAATGCTGAAATCGTCGCCGCTGGCATGAGCGGCACCGTCAAACTTCACGAAGGCTTTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACtt                                                                                    <  8:293508/148‑1 (MQ=255)
                                                                     tCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAAc                                                                                >  2:137163/1‑149 (MQ=255)
                                                                          cgtgccgGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTgg                                                                           <  5:199270/149‑1 (MQ=255)
                                                                          cgtgccgGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTgg                                                                           <  6:99866/149‑1 (MQ=255)
                                                                           gtgccgGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGc                                                                          <  3:161919/149‑1 (MQ=255)
                                                                           gtgccgGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTAACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTgg                                                                           >  4:81377/1‑148 (MQ=255)
                                                                                ggCACTGTTACCAATGCTGAAATAGGCGCCGGTGGCCTCCGGGGCACCGTCAAACGTCCCGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCgg                                                                     <  7:202143/149‑1 (MQ=255)
                                                                                    cTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGa                                                                                                  >  7:279794/1‑116 (MQ=255)
                                                                                    cTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGa                                                                                                  <  8:279794/116‑1 (MQ=255)
                                                                                       ttACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCg                                                              >  8:273068/1‑149 (MQ=255)
                                                                                                  gAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATgggc                                                    >  6:209369/1‑147 (MQ=255)
                                                                                                   aaaTAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATgc                                                                                               <  8:169542/104‑1 (MQ=255)
                                                                                                   aaaTAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATgc                                                                                               >  7:169542/1‑104 (MQ=255)
                                                                                                    aaTAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAAt                                                  <  3:81377/148‑1 (MQ=255)
                                                                                                        gTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGc                                             <  7:273068/149‑1 (MQ=255)
                                                                                                         tCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGaa                                                   <  5:167332/142‑1 (MQ=255)
                                                                                                         tCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGaa                                                   >  6:167332/1‑142 (MQ=255)
                                                                                                                       aGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGt                              >  8:251732/1‑149 (MQ=255)
                                                                                                                        gCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGt                              >  8:61932/1‑148 (MQ=255)
                                                                                                                            cACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTg                         <  1:6425/149‑1 (MQ=255)
                                                                                                                               cGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACa                      >  3:7367/1‑149 (MQ=255)
                                                                                                                                gTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACAt                     >  2:187593/1‑149 (MQ=255)
                                                                                                                                 tCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCgg                               >  6:258485/1‑138 (MQ=255)
                                                                                                                                 tCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCgg                               <  5:258485/138‑1 (MQ=255)
                                                                                                                                  cAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGAcgc                                 <  4:217838/135‑1 (MQ=255)
                                                                                                                                  cAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGAcgc                                 >  3:217838/1‑135 (MQ=255)
                                                                                                                                    aaCTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTg                                                                            >  8:182170/1‑90 (MQ=255)
                                                                                                                                    aaCTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTg                                                                            <  7:182170/90‑1 (MQ=255)
                                                                                                                                       ttCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGt               >  3:3505/1‑148 (MQ=255)
                                                                                                                                          aCGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGTCAc            <  3:260320/148‑1 (MQ=255)
                                                                                                                                                  aTTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGa                                                    <  4:146879/100‑1 (MQ=255)
                                                                                                                                                  aTTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGa                                                    >  3:146879/1‑100 (MQ=255)
                                                                                                                                                  aTTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGTCACCGGGATTGa   <  7:61932/149‑1 (MQ=255)
                                                                                                                                                   ttGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGTCACCGGGATTGAc  <  4:7367/149‑1 (MQ=255)
                                                                                                                                                   ttGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGTCACCGGGATTGAc  <  1:187593/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TAATCAACCGAGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGTCACCGGGATTGAC  >  NC_002947/2133186‑2133481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑