| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,133,336 | T→C | 8.2% | D90G (GAC→GGC) | fimI ← | type I pili subunit FimI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,133,336 | 0 | T | C | 8.2% | 169.9 / 4.9 | 61 | D90G (GAC→GGC) | fimI | type I pili subunit FimI |
| Reads supporting (aligned to +/- strand): ref base T (31/25); new base C (2/3); total (33/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCGAGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGTCACCGGGATTGAC > NC_002947/2133192‑2133481 | aCCGAGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTc > 5:240461/1‑149 (MQ=255) gAGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGACAATGCCTTCgtg < 8:11460/149‑3 (MQ=255) ttCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGAt > 5:165465/1‑148 (MQ=255) cACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGAtgctg < 6:240461/149‑1 (MQ=255) aCCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCAGGCACTGTTACCAATGCTGAAATAGTCGCAGCTGGCATCAGCGGCACCGACAAACTTCACGAAGGCAATGTATTCGCTGAagctg > 7:293508/1‑148 (MQ=255) ccGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCa < 6:165465/149‑1 (MQ=255) ccGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCa < 2:84396/149‑1 (MQ=255) cGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGAt > 1:251421/1‑142 (MQ=255) cGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGAt < 2:251421/142‑1 (MQ=255) ggTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGAc > 4:161919/1‑149 (MQ=255) gATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGttt > 8:206574/1‑149 (MQ=255) aTCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGtttt > 7:119983/1‑149 (MQ=255) tCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTg > 7:256750/1‑149 (MQ=255) gATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTc < 8:119983/149‑1 (MQ=255) gATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCAGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCAACGTCAAACTTAACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTc > 5:12791/1‑149 (MQ=255) ccTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCggg > 2:6425/1‑148 (MQ=255) cTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTa > 1:46665/1‑149 (MQ=255) ttGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGTACCGTCAAACTTCACGAAGACAATGCCTTCg < 3:233516/114‑1 (MQ=255) ttGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGTACCGTCAAACTTCACGAAGACAATGCCTTCg > 4:233516/1‑114 (MQ=255) gtgccTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTCCCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATg < 8:277627/145‑1 (MQ=255) gTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGACAATGCCTTCgtga < 2:289567/115‑4 (MQ=255) gTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGACAATGCCTTCgtga > 1:289567/1‑112 (MQ=255) gATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCCGAGGCACCGTCAAACTTCACGAAGGCATTGTCTTAGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTaa > 4:261472/1‑146 (MQ=255) tCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCa > 5:99866/1‑149 (MQ=255) agaTGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACg > 2:278416/1‑148 (MQ=255) gaTGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGa > 4:260320/1‑149 (MQ=255) ttttCTGCCGTGACTGAACTGTTACCAATGCTGAAATCGTCGCCGCTGGCATGAGCGGCACCGTCAAACTTCACGAAGGCTTTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACtt < 8:293508/148‑1 (MQ=255) tCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAAc > 2:137163/1‑149 (MQ=255) cgtgccgGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTgg < 6:99866/149‑1 (MQ=255) cgtgccgGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTgg < 5:199270/149‑1 (MQ=255) gtgccgGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGc < 3:161919/149‑1 (MQ=255) gtgccgGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTAACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTgg > 4:81377/1‑148 (MQ=255) ggCACTGTTACCAATGCTGAAATAGGCGCCGGTGGCCTCCGGGGCACCGTCAAACGTCCCGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCgg < 7:202143/149‑1 (MQ=255) cTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGa > 7:279794/1‑116 (MQ=255) cTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGa < 8:279794/116‑1 (MQ=255) ttACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCg > 8:273068/1‑149 (MQ=255) gAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATgggc > 6:209369/1‑147 (MQ=255) aaaTAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATgc < 8:169542/104‑1 (MQ=255) aaaTAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATgc > 7:169542/1‑104 (MQ=255) aaTAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAAt < 3:81377/148‑1 (MQ=255) gTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGc < 7:273068/149‑1 (MQ=255) tCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGaa < 5:167332/142‑1 (MQ=255) tCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGaa > 6:167332/1‑142 (MQ=255) aGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGt > 8:251732/1‑149 (MQ=255) gCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGt > 8:61932/1‑148 (MQ=255) cACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTg < 1:6425/149‑1 (MQ=255) cGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACa > 3:7367/1‑149 (MQ=255) gTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACAt > 2:187593/1‑149 (MQ=255) tCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCgg > 6:258485/1‑138 (MQ=255) tCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCgg < 5:258485/138‑1 (MQ=255) cAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGAcgc < 4:217838/135‑1 (MQ=255) cAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGAcgc > 3:217838/1‑135 (MQ=255) aaCTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTg > 8:182170/1‑90 (MQ=255) aaCTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTg < 7:182170/90‑1 (MQ=255) ttCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGt > 3:3505/1‑148 (MQ=255) aCGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGTCAc < 3:260320/148‑1 (MQ=255) aTTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGa < 4:146879/100‑1 (MQ=255) aTTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGa > 3:146879/1‑100 (MQ=255) aTTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGTCACCGGGATTGa < 7:61932/149‑1 (MQ=255) ttGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGTCACCGGGATTGAc < 4:7367/149‑1 (MQ=255) ttGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGTCACCGGGATTGAc < 1:187593/149‑1 (MQ=255) | ACCGAGGTTCCACCGGGTTTGATCGATGCCTTGGACTTGTCCCTGATCACAATCGAGATGTTTTCTGCCGTGCCGGCACTGTTACCAATGCTGAAATAGTCGCCGCTGGCATCAGCGGCACCGTCAAACTTCACGAAGGCATTGTCTTCGCTGATGCTGCAGACTGTTTTGTCGGGTACGCGGATGTTAAAAGATGCGCCACGGACTTCAACGTTGGCAGCGGGGAATCGCCCCATGGGAATTTCGCCCATGGCGACGCGGTCACTGACATTGCCGTCACCGGGATTGAC > NC_002947/2133192‑2133481 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |