Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,134,271 A→C 9.5% intergenic (‑37/+32) PP_1892 ← / ← fadE hypothetical protein/medium‑long chain acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,134,2710AC 9.5% 121.0 / 4.1 42intergenic (‑37/+32)PP_1892/fadEhypothetical protein/medium‑long chain acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (20/17);  new base C (2/2);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GGAACAGGGCGTGGCTTTCTTCTGGCACCTGCTCTGCTTCACCCAGCGCCACCAGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGCATCGACATCGATCACCTTGCGCCGAGCCTGTTCGGCCTCGTGCAGCCGCTGGCCATCGTCGG  >  NC_002947/2134123‑2134412
                                                                                                                                                    |                                                                                                                                             
ggAACAGGGCGTGGCTTTCTTCTGGCACCTGCTCTGCTTCACCCAGCGCCACCAGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGTAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGa                                                                                                                                               <  1:47499/149‑1 (MQ=255)
ggAACAGGGCGTGGCTTTCTTCTGGCACCTGCTCTGCTTCACCCAGCGCCACCAGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGa                                                                                                                                               <  8:216019/149‑1 (MQ=255)
 gAACAGGGCGTGGCTTTCTTCTGGCACCTGCTCTGCTTCACCCAGCGCCACCAGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAg                                                                                                                                              >  2:136816/1‑149 (MQ=255)
        gCGTGGCTTTCTTCTGGCACCTGCTCTGCTTCACCCAGCGCCACCAGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATTTTCCAGGTAGGGGTTGGCCATGGAATGGGTCGTCGGTATGGCTGAAACGGGGGGCGGCGtataata                                                                                                                                       >  7:198648/1‑146 (MQ=255)
        gCGGGGCTTTCTTCTGGCACCTGCTCTGCTTCACCCAGCGCCACCAGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGCGTATAc                                                                                                                                         <  8:198648/147‑1 (MQ=255)
                   ttctGGCACCTGCTCTGCTTCACCCAGCGCCACCAGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGtt                                                                                                                            >  4:181930/1‑149 (MQ=255)
                      tGGCACCTGCTCTGCTTCACCCAGCGCCACCAGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTtct                                                                                                                         >  7:251540/1‑149 (MQ=255)
                            ctgctctgctTCACCCAGCGCCACCAGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGaa                                                                                                                   <  6:221227/149‑1 (MQ=255)
                               ctctgctTCACCCAGCGCCACCAGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAgg                                                                                                                 >  3:16217/1‑148 (MQ=255)
                                        aCCCAGCGCCACCAGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCAc                                                                                                       <  3:134119/149‑1 (MQ=255)
                                        aCCCAGCGCCACCAGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCAc                                                                                                       <  4:187058/149‑1 (MQ=255)
                                            aGCGCCACCAGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGTGGGGCGGa                                                                                                                                               <  7:241897/105‑1 (MQ=255)
                                            aGCGCCACCAGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGa                                                                                                                                               >  8:241897/1‑105 (MQ=255)
                                                  accaGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGt                                                                                             <  3:181930/149‑1 (MQ=255)
                                                    caGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTAc                                                                                           >  5:31646/1‑149 (MQ=255)
                                                     aGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACg                                                                                          >  4:122118/1‑149 (MQ=255)
                                                                            cAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGaa                                                                   <  8:251540/149‑1 (MQ=255)
                                                                             aGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAg                                                                  <  3:122118/149‑1 (MQ=255)
                                                                                    ggTGATTTTGCAGGTTGGGTTTGGCAATGGAATGGGTCGTCGGTTTGTGTTAAAGGGGGGGTGGTGTTTAGGCCGCGGCCCGTTTGTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAAGTGGGCCTTGTGGAAGGcttcg                                                            <  4:16217/148‑1 (MQ=255)
                                                                                        atatTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGcatcgacat                                                        <  4:217322/148‑1 (MQ=255)
                                                                                        atatTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGcatcgacat                                                        <  6:31646/148‑1 (MQ=255)
                                                                                         tatTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGTTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGcatcgacatcg                                                      <  1:136816/149‑1 (MQ=255)
                                                                                         tatTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGcttcgacatcg                                                      <  6:83420/149‑1 (MQ=255)
                                                                                            tCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAg                                                                  <  6:273544/134‑1 (MQ=255)
                                                                                            tCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAg                                                                  >  5:273544/1‑134 (MQ=255)
                                                                                              cAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTgc                                                                              <  5:197446/120‑1 (MQ=255)
                                                                                              cAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTgc                                                                              >  6:197446/1‑120 (MQ=255)
                                                                                                ggTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGCATCGACATCGATCACCt                                               >  6:178562/1‑149 (MQ=255)
                                                                                                      ggTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGCATCGACATCGATCACCTTGCGcc                                         >  4:105666/1‑149 (MQ=255)
                                                                                                            ccATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGcatc                                                             >  5:131265/1‑123 (MQ=255)
                                                                                                            ccATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGcatc                                                             <  6:131265/123‑1 (MQ=255)
                                                                                                              aTGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGCATCGACATCGATCACCTTGCGCCGAGCCTGt                                 >  8:229072/1‑149 (MQ=255)
                                                                                                                  aaTGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGCATCGACATCGATCACCTTGCGCCGAGCCTGTTCgg                             >  4:143703/1‑149 (MQ=255)
                                                                                                                   aTGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGcatc                                                             >  4:177148/1‑116 (MQ=255)
                                                                                                                   aTGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGcatc                                                             <  3:177148/116‑1 (MQ=255)
                                                                                                                   aTGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGCATCGACATCGATCACCTTGCGCCGAGCCTGTTCGGc                            >  3:153579/1‑149 (MQ=255)
                                                                                                                             ggTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGCATCGACATCGATCACCTTGCGCCGAGCCTGTTCGGCCTCGTGCa                    <  7:229072/147‑1 (MQ=255)
                                                                                                                             ggTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGCATCGACATCGATCACCTTGCGCCGAGCCTGTTCGGCCTCGTGCAGc                  >  3:271640/1‑149 (MQ=255)
                                                                                                                                       aaaCGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGCATCGACATCGATCACCTTGCGCCGAGCCTGTTCGGCCTCGTGCAGCCGCTGGcc          <  4:271640/147‑1 (MQ=255)
                                                                                                                                       aaaCGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTAGCCCGGTACGGCGAGCAACTGAGCCTAGTCGAAGGCATCGACATCGATCACCTTGCGCAGAGCCTCTTCGGCATCGTGCAGCCGCTGGACAt        >  7:266613/1‑149 (MQ=255)
                                                                                                                                        aaCGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTTCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGCATCGACATCGATCACCTTGCGCCGAGCCTGTTCGGCCTCGTg                      <  2:248001/134‑1 (MQ=255)
                                                                                                                                        aaCGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGCATCGACATCGATCACCTTGCGCCGAGCCTGTTCGGCCTCGTg                      >  1:248001/1‑134 (MQ=255)
                                                                                                                                           ggggggCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGCATCGACATCGATCACCTTGCGCCGAGCCTGTTCGGCCTCGTGCAGCCGCTGGCCAtcgtc    >  2:175363/1‑149 (MQ=255)
                                                                                                                                            gggggCGGCGTATACTCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGCATCGACATCGATCACCTTGCGCCGAGCCTGTTCGGCCTCGTg                      <  5:42540/130‑1 (MQ=255)
                                                                                                                                            gggggCGGCGTATACTCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGCATCGACATCGATCACCTTGCGCCGAGCCTGTTCGGCCTCGTg                      >  6:42540/1‑130 (MQ=255)
                                                                                                                                             ggggCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGCATCGACATCGATCACCTTGCGCCGAGCCTGTTCGGCCTCGTGCAGCCGCTGGCCATCGTCgg  >  4:261387/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
GGAACAGGGCGTGGCTTTCTTCTGGCACCTGCTCTGCTTCACCCAGCGCCACCAGAATGGTGCGCAGGTGCTGGAGCAGTTGCAGGTGATATTCCAGGTAGGGGTTGGCCATGGAATGGGTCCTCGGTATGGCTGAAACGGGGGGCGGAGTATACGCCGCCCCCCGTTTCTCGCGAAGGGTCAGCGCACCTTGCCCGGTACGGCGAGCAACTGCGCCTTGTCGAAGGCATCGACATCGATCACCTTGCGCCGAGCCTGTTCGGCCTCGTGCAGCCGCTGGCCATCGTCGG  >  NC_002947/2134123‑2134412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑