Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,144,372	C→A	10.3%	L241M (CTG→ATG)	lpxK →	tetraacyldisaccharide 4'‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,144,372	0	C	A	10.3%	171.6 / 8.0	58	L241M (CTG→ATG)	lpxK	tetraacyldisaccharide 4'‑kinase
Reads supporting (aligned to +/- strand):  ref base C (28/24);  new base A (3/3);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

GCTTGCGTGAGGCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTGGTC  >  NC_002947/2144226‑2144518                                                                                                                                                       |                                                                                                                                                   gCTTGCGTGAGGCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTg                                                                                                                                                  >  2:247740/1‑149 (MQ=255)  cTTGCGTGAGGCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGGCTGc                                                                                                                                                 <  3:72236/149‑1 (MQ=255)       gTGAGGCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCg                                                                                                                                            >  6:197318/1‑149 (MQ=255)        tGAGGCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCgg                                                                                                                                           >  8:270369/1‑149 (MQ=255)          ttgCTGACGCGGGGCTGTTCAACGGCGCCAGGGTAGACCGCACCGATTGTTTCGGGTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGGGAGCGCCGT‑‑‑GCGGTCGAACATTTTCCCCGCCGGCCAGCGCCTGCATGCGGt                                                                                                                                          <  1:28322/147‑1 (MQ=255)           ggCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCACCGGCCACCGCATGCAGGCGCTgg                                                                                                                                        >  3:303859/1‑149 (MQ=255)               gACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCgg                                                                                                                                    <  1:247740/149‑1 (MQ=255)                aCGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCCGCGCCTGCATGCGGTGGCCgg                                                                                                                                    >  2:269011/1‑148 (MQ=255)                 cgcgGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCACCGCATGCAGGCGCTGGCCggcg                                                                                                                                  <  4:41778/149‑3 (MQ=255)                  gcgGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCACCGGCCACCGCATGCAGGCGCTGGCCggcgg                                                                                                                                 <  4:303859/149‑4 (MQ=255)                    ggTGCTGTTCAACGGCGCCAGCGAAGACCGGACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCg                                                                                                                               >  1:26437/1‑149 (MQ=255)                               aCGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCg                                                                                                                               <  4:208561/138‑1 (MQ=255)                               aCGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCg                                                                                                                               >  3:208561/1‑138 (MQ=255)                                      cAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTttct                                                                                                             >  1:297593/1‑149 (MQ=255)                                       aGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCgg                                                                                                                              <  1:91769/131‑1 (MQ=255)                                       aGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCgg                                                                                                                              >  2:91769/1‑131 (MQ=255)                                       aGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTttctt                                                                                                            <  3:129932/149‑1 (MQ=255)                                        gCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTttcttc                                                                                                           <  3:16846/149‑1 (MQ=255)                                        gCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTttcttc                                                                                                           <  2:34674/149‑1 (MQ=255)                                          gAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCaa                                                                                                         <  2:297593/149‑1 (MQ=255)                                                       aTGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTgggg                                                                                            >  6:235838/1‑149 (MQ=255)                                                         ggtttcggtttcTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGc                                                                                           >  5:216740/1‑148 (MQ=255)                                                         ggtttcggtttcTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGc                                                                                           >  1:95911/1‑148 (MQ=255)                                                             tcggtttcTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCACCGGCCACCGCATGCAGGCg                                                                                                                                            >  8:246060/1‑95 (MQ=255)                                                             tcggtttcTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCACCGGCCACCGCATGCAGGCg                                                                                                                                            <  7:246060/95‑1 (MQ=255)                                                                gtttcTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACAcc                                                                                                     <  8:231113/131‑1 (MQ=255)                                                                gtttcTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TATCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACAcc                                                                                                     >  7:231113/1‑131 (MQ=255)                                                                         ggcAGCGGTCGGGCCTTGTCACCGTTGGGACTGGCGAGTGCCGTCTACCCCGCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCg                                                                             <  1:269011/148‑1 (MQ=255)                                                                           cAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGcc                                                                        <  1:87784/149‑1 (MQ=255)                                                                                    gCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCg                                                                                                                               >  7:216658/1‑85 (MQ=255)                                                                                    gCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCg                                                                                                                               <  8:216658/85‑1 (MQ=255)                                                                                      ccTGGTCAACGTGCGCACTGGCGAGCGGCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCg                                                             <  7:270369/149‑1 (MQ=255)                                                                                          gTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGa                                                         >  3:139525/1‑149 (MQ=255)                                                                                              aCGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCAt                                                     <  5:235838/149‑1 (MQ=255)                                                                                                    gcACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATc                                                                   >  2:192711/1‑129 (MQ=255)                                                                                                    gcACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATc                                                                   <  1:192711/129‑1 (MQ=255)                                                                                                      aCTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTCCCGCATCCCTTCGCCGACCATGCGCAGtt                                             >  3:91909/1‑149 (MQ=255)                                                                                                          gCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCg                                                          <  5:130964/132‑1 (MQ=255)                                                                                                          gCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCg                                                          >  6:130964/1‑132 (MQ=255)                                                                                                            gAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGc                                       >  7:160528/1‑149 (MQ=255)                                                                                                              gcgcCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACGCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGccc                                     <  4:139525/149‑1 (MQ=255)                                                                                                               cgcCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCg                                    <  2:26437/149‑1 (MQ=255)                                                                                                                  cGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAg                                 >  4:244563/1‑149 (MQ=255)                                                                                                                  cGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGAGCTCAACTGGCAGCCGGTGCCTCAAACCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAg                                 >  4:13831/1‑149 (MQ=255)                                                                                                                          gcTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTgg                            >  1:16543/1‑149 (MQ=255)                                                                                                                           cTCGACCT‑TTTCCGCGCCGTCCAGCGCCTGCATGCTGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGc                           <  4:91909/149‑1 (MQ=255)                                                                                                                               aCCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCtt                        >  4:137042/1‑148 (MQ=255)                                                                                                                               aCCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCtt                        >  4:154855/1‑148 (MQ=255)                                                                                                                                ccA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGtt                                             >  2:111793/1‑126 (MQ=255)                                                                                                                                ccA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGtt                                             <  1:111793/126‑1 (MQ=255)                                                                                                                                 cA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAg                     <  8:160528/149‑1 (MQ=255)                                                                                                                                      tCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCg                 >  3:222358/1‑149 (MQ=255)                                                                                                                                        cccGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGc                >  3:142224/1‑148 (MQ=255)                                                                                                                                         ccgccgGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCa              >  7:285831/1‑149 (MQ=255)                                                                                                                                           accgGCCACCGCATGCAGGCGCTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACt            >  1:132996/2‑149 (MQ=255)                                                                                                                                            ccgGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTg           >  2:222889/1‑149 (MQ=255)                                                                                                                                                   gcgcCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTgg    <  2:110948/149‑1 (MQ=255)                                                                                                                                                     gcCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTGGTc  <  6:331499/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                   GCTTGCGTGAGGCTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGT‑‑‑GCGCTCGACCA‑TTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTGGTC  >  NC_002947/2144226‑2144518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑