Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,177,167 G→A 8.1% A21A (GCG→GCA)  PP_5485 → GNAT family acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,177,1670GA 8.1% 137.3 / 2.5 50A21A (GCG→GCA) PP_5485GNAT family acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (21/24);  new base A (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.95e-01

TTACGGGACGTCATAAAACTGGAAAAAGCCCGCGCTCTGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCTTTCACCGGGGAGAAACGGAGCTGGGATACGGGGGCATTGAAGGAAGCGGTCGGACTCGTCTGATT  >  NC_002947/2177026‑2177296
                                                                                                                                             |                                                                                                                                 
ttACGGGACGTCATAAAACTGGAAAAAGCCCGCGCTCTGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGa                                                                                                                            >  5:135967/1‑149 (MQ=255)
ttACGGGACGTCATAAAACTGGAAAAAGCCCGCGCTCTGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGCTACCGATCAAATCCAAGGCATAACTCCCCATCTg                                                                                                                             >  3:112589/1‑148 (MQ=255)
 tACGGGACGTCATAAAACTGGAAAAAGCCCGCGCTCTGGGTCATATACTGGGAGTCGATACCACACCCAGAGTCGCCAATGAACAAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGAc                                                                                                                           >  8:167735/1‑149 (MQ=255)
         gTCATAAAACTGGAAAAAGCCCGCGCTCTGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCgg                                                                                                                   <  7:104870/149‑1 (MQ=255)
            aTAAAACTGGAAAAAGCCCGCGCTCTGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACtgctgc                                                                                                                     >  1:112453/1‑144 (MQ=255)
            aTAAAACTGGAAAAAGCCCGCGCTCTGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACtgctgc                                                                                                                     <  2:112453/144‑1 (MQ=255)
            aTAAAACTGGAAAAAGCCCGCGCTCTGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCt                                                                                                                >  5:323404/1‑149 (MQ=255)
               aaaCTGGAAAAAGCCCGCGCTCTGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGc                                                                                                             <  2:286792/149‑1 (MQ=255)
                   tGGAAAAAGCCCGCGCTCTGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGAc                                                                                                         >  8:191751/1‑149 (MQ=255)
                          aGCCCGCGCTCTGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGAc                                                                                                  <  7:191751/149‑1 (MQ=255)
                             ccGCGCTCTGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGa                                                                                                   >  1:218883/1‑145 (MQ=255)
                             ccGCGCTCTGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGa                                                                                                   <  2:218883/145‑1 (MQ=255)
                             ccGCGCTCTGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCtt                                                                                               >  6:190131/1‑149 (MQ=255)
                              cgcgCTCTGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCttt                                                                                              <  4:74559/149‑1 (MQ=255)
                               gcgcTCTGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCtttt                                                                                             <  8:256283/149‑1 (MQ=255)
                                  ctctGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCa                                                                                          <  6:323404/149‑1 (MQ=255)
                                     tGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGACTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGcc                                                                                       >  1:79558/1‑149 (MQ=255)
                                      gtgtacTATACTGGGCGTCTATACCACCCCCAGAGTCTCCAATGATCCAGTCTGATATCTCTGTCCTATTGCTTGGTACAGATCAAATCCTAGGCCTAAATGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGcc                                                                                       <  4:112589/143‑1 (MQ=255)
                                       ggTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGcc                                                                                       <  6:135967/147‑1 (MQ=255)
                                        gTCCTATACTTGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCg                                                                                     <  5:190131/148‑1 (MQ=255)
                                         tCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGt                                                                                   >  1:213367/1‑149 (MQ=255)
                                                cTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGt                                                                            >  6:71430/1‑149 (MQ=255)
                                                cTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCACATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGt                                                                            >  2:304703/1‑149 (MQ=255)
                                                cTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCACATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGt                                                                            >  2:299942/1‑149 (MQ=255)
                                                 tGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATc                                                                                                                               <  5:19846/97‑1 (MQ=255)
                                                 tGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATc                                                                                                                               >  6:19846/1‑97 (MQ=255)
                                                   ggCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGtt                                                                           <  5:71430/147‑1 (MQ=255)
                                                   ggCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCACATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGtt                                                                           <  1:304703/147‑1 (MQ=255)
                                                   ggCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCACATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGtt                                                                           <  1:299942/147‑1 (MQ=255)
                                                     cGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTAt                                                                       <  5:324604/149‑1 (MQ=255)
                                                      gTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTAtt                                                                      >  7:176122/1‑149 (MQ=255)
                                                         gATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTcc                                                                    <  4:309130/148‑1 (MQ=255)
                                                         gATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTAGCAGCCCGGTCGAATGTTCTATTCCt                                                                   >  2:217828/1‑149 (MQ=255)
                                                                           gCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCTTTCACCGGGGAGAAACgg                                                 <  8:176122/149‑1 (MQ=255)
                                                                                     cAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGAccc                                                                                                       <  5:212063/85‑1 (MQ=255)
                                                                                     cAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGAccc                                                                                                       >  6:212063/1‑85 (MQ=255)
                                                                                     cAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCTTTCACCGGGGAGAAACGGAGCTGGGAt                                        >  8:284977/1‑148 (MQ=255)
                                                                                       gtgtGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCTTTCACCGGGGAGAAACGGAGCTGGGATACg                                     <  7:167735/146‑1 (MQ=255)
                                                                                                     tCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCTTTCACCGGGGAGAAACGGAGCTGGGATACGGGGGCATTgaa                          <  7:284977/146‑1 (MQ=255)
                                                                                                        gATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCTTTCACCGGGGAGAAACGGAGCTGGGATACGGGGGCATTgaagg                        <  4:132652/145‑1 (MQ=255)
                                                                                                        gATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCTTTCACCGGGGAGAAACGGAGCTGGGATACGGGGGCATTgaagg                        >  3:132652/1‑145 (MQ=255)
                                                                                                         aTTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCTTTCACCGGGGAGAAACGGAGCTGGGATACGGGGGCATTGAAGGAAGCg                   >  3:174832/1‑149 (MQ=255)
                                                                                                                      gATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCTTTCACCGGGGAGAAACGGAGCTGGGATACGGGGGCATTGAAGGAAGCGGTCGGACTCGTCt      >  6:276193/1‑149 (MQ=255)
                                                                                                                      gATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCTTTCACCGGGGAGAAACGGAGCTGGGATACGGGGGCATTGAAGGAAGCGGTCGGACTCGTCt      <  5:276193/149‑1 (MQ=255)
                                                                                                                          aaaTCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCttt                                                                 <  6:34790/86‑1 (MQ=255)
                                                                                                                          aaaTCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCttt                                                                 >  5:34790/1‑86 (MQ=255)
                                                                                                                           aaTCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCTTTCACCGGGGAGAAACGGAGCTGGGATACGGGGGCATTGAAGGAAGCGGTCGGACTCGTCTGAtt  >  2:292214/1‑148 (MQ=255)
                                                                                                                           aaTCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCTTTCACCGGGGAGAAACGGAGCTGGGATACGGGGGCATTGAAGGAAGCGGTCGGACTCGTCTGAtt  <  1:292214/148‑1 (MQ=255)
                                                                                                                                     cTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCTTTCACCGGGGAGAAACGGa                                                <  6:113203/92‑1 (MQ=255)
                                                                                                                                     cTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCTTTCACCGGGGAGAAACGGa                                                >  5:113203/1‑92 (MQ=255)
                                                                                                                                             |                                                                                                                                 
TTACGGGACGTCATAAAACTGGAAAAAGCCCGCGCTCTGGGTCCTATACTGGGCGTCGATACCACCCCCAGAGTCGCCAATGAACCAGTCTGATATCTCTGTCCGATTGCTTGGTACCGATCAAATCCAAGGCCTAACTGCGCATCTGACTGCTGCGGGCTTGCCGACCCAAGACCTTTTGCAGCCCGGTCGAATGTTCTATTCCTTTCACCGGGGAGAAACGGAGCTGGGATACGGGGGCATTGAAGGAAGCGGTCGGACTCGTCTGATT  >  NC_002947/2177026‑2177296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑