Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,235,837 G→C 10.0% P316P (CCG→CCC)  uvrB → uvrABC excinuclease protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,235,8370GC10.0% 143.3 / 6.8 50P316P (CCG→CCC) uvrBuvrABC excinuclease protein B
Reads supporting (aligned to +/- strand):  ref base G (21/24);  new base C (2/3);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

GAGCGTCTGGAGTACCTGCACAAGGCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAGGGCGACCGTTCACGCAAGGAGACCCTGGTCGAGTACGGCTTCC  >  NC_002947/2235691‑2235985
                                                                                                                                                  |                                                                                                                                                    
gAGCGTCTGGAGTACCTGCACAAGGCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCccggc                                                                                                                                                    >  4:215963/1‑149 (MQ=255)
  gCGTCTGGAGTACCTGCACAAGGCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCccggccg                                                                                                                                                  >  8:39685/1‑149 (MQ=255)
  gCGTCTGGAGTACCTGCACAAGGCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCccggccg                                                                                                                                                  <  7:101739/149‑1 (MQ=255)
     tCTGGAGTACCTGCACAAGGCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCgggg                                                                                                                                               <  7:130739/149‑1 (MQ=255)
        ggAGTACCTGCACAAGGCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGgcgc                                                                                                                                            >  7:146189/1‑149 (MQ=255)
         gAGTACCTGCACAAGGCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGcgcc                                                                                                                                           >  5:241932/1‑149 (MQ=255)
         gAGTACCTGCACAAGGCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGcgcc                                                                                                                                           <  6:241932/149‑1 (MQ=255)
          aGTACCTGCACAAGGCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCCGGCCGGGcgccc                                                                                                                                          <  6:281069/149‑2 (MQ=255)
               cTGCACAAGGCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCCGGCGCCCCGGCCGGGcgcccggaca                                                                                                                                     <  2:306352/149‑7 (MQ=255)
                 gCACAAGGCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCa                                                                                                                                   >  3:215056/1‑149 (MQ=255)
                  cacaAGGCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCAc                                                                                                                                  >  8:112151/1‑149 (MQ=255)
                   acaAGGCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACACGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCAcc                                                                                                                                 >  3:258696/1‑149 (MQ=255)
                    caAGGCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCAccc                                                                                                                                >  2:28418/1‑149 (MQ=255)
                     aaGGCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCt                                                                                                                               <  3:183634/149‑1 (MQ=255)
                       ggCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGt                                                                                                                             <  3:215963/149‑1 (MQ=255)
                        gCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTa                                                                                                                            <  5:83301/149‑1 (MQ=255)
                         gcaacaaGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTAc                                                                                                                           >  2:263984/2‑149 (MQ=255)
                          caacaaGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTAc                                                                                                                           >  6:265275/1‑148 (MQ=255)
                              aaGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCCGGCGCCCCGGCCGGGcgcccg                                                                                                                                         >  3:141428/1‑128 (MQ=255)
                              aaGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCCGGCGCCCCGGCCGGGcgcccg                                                                                                                                         <  4:141428/130‑3 (MQ=255)
                               aGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTAc                                                                                                                     <  1:305716/149‑1 (MQ=255)
                                    gTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGACGCCGCCGCCCACCCTGTACGATTACCTgc                                                                                                                 <  1:28418/148‑1 (MQ=255)
                                             cAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATgc                                                                                                       >  4:307044/1‑149 (MQ=255)
                                               gCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATgcgc                                                                                                     <  4:215056/149‑1 (MQ=255)
                                                      gAGCAACGCACCCGCTTCGTTCTGGCGATTATCCTCGAGGTGGGATACTGCAACGGCATCTAAAACTACTCGGGTTACCTGTCCGGGCGCCGGGCGGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGt                                                                                              <  4:307422/149‑1 (MQ=255)
                                                      gAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGTCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGctgctg                                                                                                <  4:258696/147‑1 (MQ=255)
                                                      gAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGctgctg                                                                                                <  8:233686/147‑1 (MQ=255)
                                                      gAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGt                                                                                              >  7:261895/1‑149 (MQ=255)
                                                       aGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTg                                                                                             <  1:203754/149‑1 (MQ=255)
                                                        gCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGa                                                                                            <  7:112151/149‑1 (MQ=255)
                                                                       cGATCTGGAGATGATCCTGGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCTCCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACTAATCacac                                                                              <  2:35252/148‑1 (MQ=255)
                                                                          tCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGt                                                                            >  8:2766/1‑147 (MQ=255)
                                                                                  tgagCCTCGAGCTGGGCTACTGCACCGGAATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTcc                                                                   <  8:146189/148‑1 (MQ=255)
                                                                                   gatCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCg                                                                  >  3:88710/1‑148 (MQ=255)
                                                                                                ggCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGcc                                                      <  4:88710/147‑1 (MQ=255)
                                                                                                 gCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATg                                                   >  6:245428/1‑149 (MQ=255)
                                                                                                        cAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGGCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATgcg                                                                                                      <  1:50761/91‑1 (MQ=255)
                                                                                                        cAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAgg                                            <  7:2766/149‑1 (MQ=255)
                                                                                                        cAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAg                                             <  5:245428/148‑1 (MQ=255)
                                                                                                        cAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCCCCGCCCCCCACCCTCTACGATTACCTGCCGCCCGATgcg                                                                                                      >  2:50761/1‑91 (MQ=255)
                                                                                                               aTCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAGGGCGACCg                                     >  8:81126/1‑149 (MQ=255)
                                                                                                                 cGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAGGGCGACCGt                                    >  5:152057/1‑148 (MQ=255)
                                                                                                                    aaaCTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAGGGCGACCGTTCAc                                >  6:229074/1‑149 (MQ=255)
                                                                                                                    aaaCTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAGGGCGACCGTTCAc                                >  5:65011/1‑149 (MQ=255)
                                                                                                                          ctCGCGTTACCTGTCCGGGCGCCGGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAGGGCGACCGTTCACGCAAg                           <  8:261895/148‑1 (MQ=255)
                                                                                                                          ctCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATcac                                                                                >  2:225024/1‑95 (MQ=255)
                                                                                                                          ctCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATcac                                                                                <  1:225024/95‑1 (MQ=255)
                                                                                                                                  aCCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAGGGCGACCGTTCACGCAAGGAGACCCTg                  >  7:42199/1‑149 (MQ=255)
                                                                                                                                  aCCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAGGGCGACCGTTCACGCAAGGAGACCCTg                  >  8:16424/1‑149 (MQ=255)
                                                                                                                                   ccTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAGGGCGACCGTTCACGCAAGGAGACCCTg                  <  7:39685/148‑1 (MQ=255)
                                                                                                                                          gggCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAGGGCGACCGTTCACGCAAGGAGACCCTGGTCGAGTa          <  7:16424/149‑1 (MQ=255)
                                                                                                                                            gcgcCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGGGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAGGGCGACCGTTCACGCAAGGAGACCCTGGTCGAGTACg        <  1:263984/149‑1 (MQ=255)
                                                                                                                                            gcgcCCCGGCCGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAGGGCGACCGTTCACGCAAGGAGACCCTGGTCGAGTACg        >  6:193143/1‑149 (MQ=255)
                                                                                                                                                ccggccggGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAGGGCGACCGTTCACGCAAGGAGACCCTGGTCGAGTACGGCtt    <  3:307044/149‑1 (MQ=255)
                                                                                                                                                  ggccggGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAGGGCGACCGTTCACGCAAGGAGACCCTGGTCGAGTACGGCTTcc  >  6:295194/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GAGCGTCTGGAGTACCTGCACAAGGCCAACAAGCTGGTCGAAGCGCAGCGTCTGGAGCAACGCACCCGCTTCGATCTGGAGATGATCCTCGAGCTGGGCTACTGCAACGGCATCGAAAACTACTCGCGTTACCTGTCCGGGCGCCCGGCCGGGGCGCCGCCGCCCACCCTGTACGATTACCTGCCGCCCGATGCGCTGCTGGTGATCGACGAATCACACGTCAGCGTTCCGCAGGTCGGCGCCATGTACAAGGGCGACCGTTCACGCAAGGAGACCCTGGTCGAGTACGGCTTCC  >  NC_002947/2235691‑2235985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑