| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,271,666 | C→T | 14.5% | intergenic (+50/‑50) | PP_t32 → / → PP_t33 | tRNA‑Asp/tRNA‑Asp |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,271,666 | 0 | C | T | 14.5% | 113.7 / 9.7 | 48 | intergenic (+50/‑50) | PP_t32/PP_t33 | tRNA‑Asp/tRNA‑Asp |
| Reads supporting (aligned to +/- strand): ref base C (22/19); new base T (4/3); total (26/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
CCAGAGAGGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTCCC > NC_002947/2271521‑2271810 | ccAGAGAGGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATg > 4:200926/1‑149 (MQ=255) cAGAGACGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGc > 1:12447/1‑149 (MQ=255) agagGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCtt < 1:188710/149‑1 (MQ=255) gAACGCACTGCGCAGCGGTAGTTCTGTCGGTTAGAATTCCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGGCTGGCACCGGTCTGAAGCCACAAGGCATGTGCCTTgtgg < 3:42444/149‑4 (MQ=25) gAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGc < 2:12447/149‑1 (MQ=255) cGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGa > 7:250647/1‑149 (MQ=255) aCTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATcc < 3:181630/149‑1 (MQ=255) cAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGttt > 8:201197/1‑149 (MQ=255) gCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCa < 8:250647/149‑1 (MQ=255) gTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGGGCCTGGCACCGGGCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATc < 8:246955/149‑1 (MQ=255) ttCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCggg < 7:201197/148‑1 (MQ=255) ctgtgggTTATAATACCGGCATGTCACGCCGTGGGTCGCGGGTTGGAGTCCGGTCCGCTGATACATTTTTCAGTAACAGATGGGTGTCTGGCACCTGTCTTAAGCCACAAGGCTCATGCCTTG‑GGCTGATCCCAGTTTCAATCggg < 1:253298/141‑1 (MQ=255) cAGTCGGTTAGAATACCGGCCTGTCACCCCGCGTGTCGCGGGTACGAGACCCGTACGCGGCGCCATTTTTCAGTAAAACAACGGTCCCAGGCACCGTTCTGAAGCCACAAGGCACATGCATAG‑GGATGAACCCAGTTTCaaaccgg > 2:253298/1‑141 (MQ=16) aGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGgcgc < 3:200926/148‑1 (MQ=255) aGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCTCCGGTCTGAAGCCACAAGGCATGTGCCTTgtg > 4:182353/1‑114 (MQ=255) aGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCATGTGCCTTgtg < 3:182353/116‑3 (MQ=255) gAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGAACCCAGTTTCAATCGGGCGCAAGCCTGaa < 1:180290/148‑1 (MQ=255) tACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGAt < 3:146024/149‑1 (MQ=255) aCCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTg < 6:294840/109‑1 (MQ=255) aCCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTg > 5:294840/1‑109 (MQ=255) cGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGACTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCACAGTTTCAATCCGGCGCAAGCCTGAACGATACg > 2:3915/1‑149 (MQ=255) gCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGTGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGtt < 8:246379/119‑1 (MQ=255) gCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGtt > 7:246379/1‑119 (MQ=255) tCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAAc > 4:207732/1‑136 (MQ=255) tCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAAc < 3:207732/136‑1 (MQ=255) cACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGTGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGAt < 1:196219/138‑1 (MQ=255) cACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGTGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGAt > 2:196219/1‑138 (MQ=255) cGTCCGCTGCGCCATTTTACAGTAAAAGATCGGTGACTGGCACCGGTCTGAAGCCACAAGGAACATGACTTG‑GGCTGATACCAGTTTCAATCGGGAGCAAGCCTGAACGATAAGCAGAGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGt > 6:290202/1‑149 (MQ=25) cTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTAGGTTAGAATA‑CCGGCCTGTAACGcc > 3:183305/1‑149 (MQ=255) cgcCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCAGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCggg > 2:135486/1‑149 (MQ=255) cATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGt > 7:35898/1‑149 (MQ=255) tttttCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTcg > 4:182205/1‑149 (MQ=255) gTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCAGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTCGCGGGTTc < 1:135486/149‑1 (MQ=255) aTGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTccc > 2:88873/1‑149 (MQ=255) ccTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCt > 3:269158/1‑149 (MQ=255) ggCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTgcg > 3:184726/1‑149 (MQ=255) aCCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCa > 2:307021/1‑149 (MQ=255) aCCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCa > 7:23753/1‑149 (MQ=255) ccGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCAt < 8:23753/149‑1 (MQ=255) aaGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGt < 1:88873/148‑1 (MQ=255) ccACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGt > 2:69453/1‑121 (MQ=255) ccACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGt < 1:69453/121‑1 (MQ=255) cccaAGGCATGTGCCTTGTGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAATGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACg < 1:160750/98‑1 (MQ=14) cccaAGGCATGTGCCTTGTGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACg > 2:160750/3‑100 (MQ=17) cccaAGGCATGTGCCTTGTGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTc > 5:255841/3‑147 (MQ=255) cacaAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAg < 1:307021/149‑1 (MQ=255) ccaAGGCATGTGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAgg > 7:20643/2‑149 (MQ=255) acaAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAgg > 2:262113/1‑149 (MQ=255) acaTGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTccc > 1:268/1‑146 (MQ=255) | CCAGAGAGGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTG‑GGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATA‑CCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTCCC > NC_002947/2271521‑2271810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |