Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,313,664 T→C 7.5% I142V (ATC→GTC)  benE‑I ← benzoate transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,313,6640TC 7.5% 150.4 / 2.1 53I142V (ATC→GTC) benE‑Ibenzoate transport protein
Reads supporting (aligned to +/- strand):  ref base T (29/20);  new base C (2/2);  total (31/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

GTACCCCAGGCCCAGCAGCAGTATGGCGACCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGT  >  NC_002947/2313518‑2313811
                                                                                                                                                  |                                                                                                                                                   
gTACCCCAGGCCCAGCAGCAGTATGGCGACCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGc                                                                                                                                                   >  8:123291/1‑149 (MQ=255)
gTACCCCAGGCCCAGCAGCAGTATGGCGACCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGc                                                                                                                                                   >  7:285477/1‑149 (MQ=255)
gTACCCCAGGCCCAGCAGCAGTATGGCGACCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGc                                                                                                                                                   >  7:282373/1‑149 (MQ=255)
     ccAGGCCCAGCAGCAGTATGGCGACCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGc                                                                                                                                               >  7:258494/1‑148 (MQ=255)
         gCCCAGCAGCAGTATGGCGACCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCcagca                                                                                                                                          <  8:285477/149‑1 (MQ=255)
           ccAGCAGCAGTATGGCGACCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAgg                                                                                                                                        >  3:147002/1‑149 (MQ=255)
             agcagcagTATGGCGACCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGcc                                                                                                                                      <  4:157186/149‑1 (MQ=255)
                  cagTATGGCGACCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCcggcgg                                                                                                                                 >  4:209409/1‑149 (MQ=255)
                    gTATGGCGACCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCgg                                                                                                                                                <  8:68374/132‑1 (MQ=255)
                    gTATGGCGACCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCgg                                                                                                                                                >  7:68374/1‑132 (MQ=255)
                      aTGGCGACCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAAt                                                                                                                             >  1:56466/1‑149 (MQ=255)
                             ccACGGCGTAACGGGCGGGGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCTGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGccgccg                                                                                                                      <  3:203093/149‑1 (MQ=255)
                              cACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCATTGCCGCCgg                                                                                                                     <  8:20561/149‑1 (MQ=255)
                               aCGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCggg                                                                                                                    >  7:164728/1‑149 (MQ=255)
                                           gCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGACGCCGGCCAGCAGGCcgg                                                                                                                                    >  1:165121/1‑121 (MQ=255)
                                           gCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGACGCCGGCCAGCAGGCcgg                                                                                                                                    >  1:170328/1‑121 (MQ=255)
                                           gCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGACGCCGGCCAGCAGGCcgg                                                                                                                                    <  2:170328/121‑1 (MQ=255)
                                           gCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGACGCCGGCCAGCAGGCcgg                                                                                                                                    <  2:165121/121‑1 (MQ=255)
                                                    cGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTc                                                                                               <  8:258494/149‑1 (MQ=255)
                                                       ttGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGaa                                                                                            >  3:179792/1‑149 (MQ=255)
                                                        tGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGaa                                                                                            <  7:123291/148‑1 (MQ=255)
                                                         gAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTAc                                                                                                              <  6:326478/129‑1 (MQ=255)
                                                         gAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTAc                                                                                                              >  5:326478/1‑129 (MQ=255)
                                                            aGCCACATAGGCTGCAATCAGCAACCCGGCGAGGCTGGGTTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCTTCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCAcc                                                                                       <  5:255053/149‑1 (MQ=255)
                                                                           aaTCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGca                                                                        >  2:117408/1‑149 (MQ=255)
                                                                             tCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGcac                                                                       >  4:32416/1‑148 (MQ=255)
                                                                                gCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCa                                                                                 <  7:171530/135‑1 (MQ=255)
                                                                                gCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCa                                                                                 >  8:171530/1‑135 (MQ=255)
                                                                                    cccGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGc                                                               <  4:69767/149‑1 (MQ=255)
                                                                                       ggCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAAt                                                                                                                             >  5:286116/1‑84 (MQ=255)
                                                                                       ggCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAAt                                                                                                                             <  6:286116/84‑1 (MQ=255)
                                                                                         cGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCcggcg                                                          <  8:164728/149‑1 (MQ=255)
                                                                                                       accgacAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTGATCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTa                                            <  4:309379/149‑1 (MQ=255)
                                                                                                       accgacAGGCTGGCAAAGGCACAGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATCCCGCCGGGCACAACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAgg                                              >  2:136871/1‑147 (MQ=255)
                                                                                                          gacAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAgg                                          >  4:316253/1‑148 (MQ=255)
                                                                                                           acAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGTAGGCCGGCGGCCATGCCGCCGGGCACTACGCGCCCCAGCCGCTCCAAGCACCCGCTCAGCCCCAGCCCCACGAAGCCGCCGCCCGACAGCAGGTAGGCa                                        >  8:255528/1‑149 (MQ=255)
                                                                                                              ggCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCt                                     <  4:179792/149‑1 (MQ=255)
                                                                                                                   gCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGAACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGc                                >  1:128628/1‑149 (MQ=255)
                                                                                                                     aaaGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCt                              >  3:151716/1‑149 (MQ=255)
                                                                                                                         gCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCt                          >  8:206241/1‑149 (MQ=255)
                                                                                                                           aCCGTTGCCGAACTTCAGCAGGTTGCCGGCCATCAGGCCGGGGGCAATGCCGCCGGGCACTACGCGCACCATCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGt                        <  7:45082/149‑1 (MQ=255)
                                                                                                                           aCCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACGACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGt                        <  4:147002/149‑1 (MQ=255)
                                                                                                                               aTGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAggg                    <  3:32416/149‑1 (MQ=255)
                                                                                                                                   cGAACTGCAGCAGGATGCCGGCCAGTAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACg                <  7:255528/149‑1 (MQ=255)
                                                                                                                                   cGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACg                >  7:119663/1‑149 (MQ=255)
                                                                                                                                   cGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACg                >  3:300298/1‑149 (MQ=255)
                                                                                                                                       cTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTgg            >  2:309397/1‑149 (MQ=255)
                                                                                                                                        tGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGc           >  3:250343/1‑149 (MQ=255)
                                                                                                                                           agcagGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAg        >  4:253151/1‑149 (MQ=255)
                                                                                                                                             cagGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAggg      >  7:27987/1‑149 (MQ=255)
                                                                                                                                              agGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGc     >  5:329999/1‑149 (MQ=255)
                                                                                                                                               ggATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATAGCCTCGCTGTAGGGCACGCTGGCCAGGGCg    <  3:209409/149‑1 (MQ=255)
                                                                                                                                                 aTGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGt  >  5:148616/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTACCCCAGGCCCAGCAGCAGTATGGCGACCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGT  >  NC_002947/2313518‑2313811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑