Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,336,498 A→T 13.0% intergenic (‑42/+38) PP_2052 ← / ← PP_5500 bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,336,4980AT13.0% 113.7 / 9.5 46intergenic (‑42/+38)PP_2052/PP_5500bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (21/19);  new base T (2/4);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01

GAGAAGCTTGCTTGAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAGGT  >  NC_002947/2336351‑2336642
                                                                                                                                                   |                                                                                                                                                
gagaAGCTTGCTTGAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCaa                                                                                                                                                 >  3:303908/1‑149 (MQ=255)
     gcttgcttGAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGc                                                                                                                                            <  3:277665/149‑1 (MQ=255)
         gcttgAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATAGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCga                                                                                                                                          <  2:211440/147‑1 (MQ=255)
          cttgAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgaga                                                                                                                                        <  6:32732/148‑1 (MQ=255)
          cttgAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGaa                                                                                                                                       >  4:11087/1‑149 (MQ=255)
          cttgAGTGTCCTGAAGCGCAGGCGCTCGGGTTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCGGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgaga                                                                                                                                        <  3:236373/148‑1 (MQ=255)
              aGTGTCATGAAGCGCAGGCGCTCGGTGTCGAAGAGTGTGCCATCCATATCGAATATGGAGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCTGGCATCTCGCCTGTTGCAGGCgagatgcc                                                                                                                                    <  5:300051/148‑5 (MQ=255)
               gtgtCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTTCACATTGTCGTTGCGTCAGGCATATCGCCTGAAACAGTCGAGAACGttg                                                                                                                                  >  8:293129/1‑149 (MQ=255)
                tgtCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtgt                                                                                                                                 >  7:76791/1‑149 (MQ=255)
                tgtCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtgt                                                                                                                                 <  1:121880/149‑1 (MQ=255)
                    cTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAAc                                                                                                                                      >  5:21084/1‑140 (MQ=255)
                    cTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAAc                                                                                                                                      <  6:21084/140‑1 (MQ=255)
                    cTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGa                                                                                                                             <  3:283360/149‑1 (MQ=255)
                     tGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtgt                                                                                                                                 >  8:281979/1‑144 (MQ=255)
                     tGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGgtgt                                                                                                                                 <  7:281979/144‑1 (MQ=255)
                     tGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATACATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTTCCCCGTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGTACGgtgtgt                                                                                                                               >  7:116686/1‑146 (MQ=255)
                       aaGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCt                                                                                                                          <  3:11087/149‑1 (MQ=255)
                         gcgcAGGCGCTCGGTGTCGAAGAGGGGGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAG‑AAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCa                                                                                                                       <  4:81491/149‑1 (MQ=255)
                               gcgcTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCTCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGTCGAGAACGGTGTGTGAGCTGCAGTggg                                                                                                                  <  3:315878/149‑1 (MQ=255)
                                   tCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTggggg                                                                                                                >  4:293654/1‑147 (MQ=255)
                                       tgtCGAATAGTGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTATACCGAGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTc                                                                                                           <  7:293129/148‑1 (MQ=255)
                                         tCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAg                                                                                                         <  8:76791/148‑1 (MQ=255)
                                            aaGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGGGTGTGAGCTGCAGTGGGTGCAGGCAGGTCg                                                                                                     >  6:107023/1‑149 (MQ=255)
                                             agagGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCgg                                                                                                    >  6:192533/1‑149 (MQ=255)
                                             agagGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCgg                                                                                                    <  1:64651/149‑1 (MQ=255)
                                                   tGCCAACCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGca                                                                                               >  2:175028/1‑148 (MQ=255)
                                                         ccatATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTc                                                                                        >  7:108056/1‑149 (MQ=255)
                                                              tCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgagat                                                                                                                                       >  5:268800/1‑96 (MQ=255)
                                                              tCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGTTGCAGGCgagat                                                                                                                                       <  6:268800/97‑2 (MQ=255)
                                                                gAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAggg                                                                                 <  4:62087/149‑1 (MQ=255)
                                                                     tGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGATGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACg                                                                            >  5:71381/1‑149 (MQ=255)
                                                                          cTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAg                                                                       <  3:293654/149‑1 (MQ=255)
                                                                          cTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGGCGGGGGCACACGTTCGACAGGGGGACGCTCAg                                                                       <  5:192533/149‑1 (MQ=255)
                                                                                     tttACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCCCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGAc                                                                <  1:175028/145‑1 (MQ=255)
                                                                                        aCCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAgg                                                         >  1:155062/1‑149 (MQ=255)
                                                                                          cGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTggggg                                                                                                                >  2:180272/1‑92 (MQ=255)
                                                                                          cGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTggggg                                                                                                                <  1:180272/92‑1 (MQ=255)
                                                                                          cGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGt                                                        >  3:44732/1‑148 (MQ=255)
                                                                                           gTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTc                                                                                        >  5:330110/1‑115 (MQ=255)
                                                                                           gTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTc                                                                                        <  6:330110/115‑1 (MQ=255)
                                                                                               aagaaaAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTGGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAggcg                                                  >  1:225803/1‑149 (MQ=255)
                                                                                                           ggCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCggg                                      >  4:106758/1‑149 (MQ=255)
                                                                                                               gTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGt                                  >  8:239222/1‑149 (MQ=255)
                                                                                                                   ccccTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAg                                                                    <  8:98916/111‑1 (MQ=255)
                                                                                                                   ccccTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAg                                                                    >  7:98916/1‑111 (MQ=255)
                                                                                                                                        ttctcGCCTGTTGCAGCCTAGAACGGTGTGTGAACTGCAGTGGGCGCAGTCAGGTCGGGCGCACACGTTCGACAGGGGGACTCTCAGGAGTCACGCAGAGGTAACGCGGGGAGGAGCGGGGCGAGAAGAGGAGGTTGGCAACCGTTgcg         >  4:9895/2‑149 (MQ=255)
                                                                                                                                               cTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAggt  >  4:76402/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GAGAAGCTTGCTTGAGTGTCCTGAAGCGCAGGCGCTCGGTGTCGAAGAGGGTGCCATCCATATCGAAGATGGCGCTGAAAATCTGTTTACCGTGAAAGAAAAGCATTGGCTGTTGCCCCTTGTCGTTGCGTCAGGCATCTCGCCTGCAACAGGCGAGAACGGTGTGTGAGCTGCAGTGGGGGCAGTCAGGTCGGGGGCACACGTTCGACAGGGGGACGCTCAGGAGTGACGCAGAGGTAAGGCGGGGCGGAGCGGGGCGTGCAGAGGAGGTTGGCAACCGTTGCGGCGAGGT  >  NC_002947/2336351‑2336642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑