Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,356,674 A→C 7.6% W50G (TGG→GGG)  PP_2070 ← AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,356,6740AC 7.6% 149.2 / 2.0 50W50G (TGG→GGG) PP_2070AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (19/27);  new base C (2/2);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

GCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGTGGCTGGCCACTTCATCGCCGTGTCCGTAGTCCCTTTCCAGTGCTTGCATG  >  NC_002947/2356527‑2356822
                                                                                                                                                   |                                                                                                                                                    
gCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCGGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCca                                                                                                                                                      >  1:164259/1‑148 (MQ=255)
  aCCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGTACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAATGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACaa                                                                                                                                                   <  4:50648/149‑1 (MQ=255)
  aCCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCcac                                                                                                                                                     >  6:38203/1‑147 (MQ=255)
   cccGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAg                                                                                                                                                  >  1:314599/1‑149 (MQ=255)
    ccGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAgg                                                                                                                                                 >  4:156047/1‑149 (MQ=255)
      gTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCa                                                                                                                                               <  2:250562/149‑1 (MQ=255)
           gccgccAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCGGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGccc                                                                                                                                          <  2:164259/149‑1 (MQ=255)
           gccgccAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGccc                                                                                                                                          >  8:201806/1‑149 (MQ=255)
               ccAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCccgccg                                                                                                                                      <  6:330109/149‑1 (MQ=255)
               ccAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCccgccg                                                                                                                                      >  7:188857/1‑149 (MQ=255)
               ccAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCccgccg                                                                                                                                      <  4:26533/149‑1 (MQ=255)
                 aGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCggg                                                                                                                                    >  1:274281/1‑149 (MQ=255)
                  ggATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATGCCTTGTGGATGCCCGCCgggg                                                                                                                                   <  1:73781/149‑1 (MQ=39)
                  ggATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATGCCTTGTGGATGCCCGCCgggg                                                                                                                                   <  1:222742/149‑1 (MQ=39)
                       aGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCAc                                                                                                                              <  2:75593/149‑1 (MQ=255)
                           cgcgCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGAGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCa                                                                                                                           <  7:34038/148‑1 (MQ=255)
                            gcgcAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCAGCCACAAGGCATGCCCGCCGGGGCTCACCCAGc                                                                                                                         >  2:177326/1‑149 (MQ=255)
                                       cGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCg                                                                                                              >  2:286558/1‑149 (MQ=255)
                                                cgaTGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGAcc                                                                                                     >  7:119635/1‑149 (MQ=255)
                                                 gaTGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGAccc                                                                                                    <  2:314599/149‑1 (MQ=255)
                                                   tGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGc                                                                                                  <  4:270644/149‑1 (MQ=255)
                                                       cTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCa                                                                                              >  6:75778/1‑149 (MQ=255)
                                                          gCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCAc                                                                                             <  3:156047/147‑1 (MQ=255)
                                                            aGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCAcgccg                                                                                         >  3:219706/1‑149 (MQ=255)
                                                                   gATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCCTCCCCAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGCCCCGCATCACGCCGCTggcgg                                                                                  >  7:254866/1‑149 (MQ=255)
                                                                      gtgGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAg                                                                                                                          <  4:168418/106‑1 (MQ=255)
                                                                      gtgGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAg                                                                                                                          >  3:168418/1‑106 (MQ=255)
                                                                               gAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCt                                                                      <  5:38203/149‑1 (MQ=255)
                                                                               gAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCt                                                                      <  8:188857/149‑1 (MQ=255)
                                                                                 gcagcaAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTgg                                                                    >  4:47284/1‑149 (MQ=255)
                                                                                       aGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTc                                                               >  8:21862/1‑148 (MQ=255)
                                                                                       aGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTc                                                               <  7:21862/148‑1 (MQ=255)
                                                                                       aGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCg                                                              <  1:286558/149‑1 (MQ=255)
                                                                                            cgcATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGt                                                          <  4:219706/148‑1 (MQ=255)
                                                                                             gcATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTgg                                                        <  3:47284/149‑1 (MQ=255)
                                                                                               aTGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCTCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAATTCAGCTGGTCTTCGGCGTGGCa                                                      <  7:201806/149‑1 (MQ=255)
                                                                                                    gACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGtggc                                                 >  2:195037/1‑149 (MQ=255)
                                                                                                        aCGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGtggctggc                                             <  2:274281/149‑1 (MQ=255)
                                                                                                                 aTGCGGATGGCATGGCTGACCCCGGCGGGCTTCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGTGGCAGGCCACTTCATc                                    >  1:27344/1‑149 (MQ=255)
                                                                                                                    tgaaaggcgtggcTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGTGGCTGGCCACTTCATCGcc                                 <  4:25726/144‑1 (MQ=255)
                                                                                                                    cGGATGGCAGGGCTGACCCCGGCGGGCATCCACAAGGCAGGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGTGGCTGGCCACTTCATCGc                                  <  1:195037/148‑1 (MQ=255)
                                                                                                                        tggcatggcTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGTGGCTGGCCACTTCATCGCCgtgt                             <  7:262250/149‑1 (MQ=255)
                                                                                                                           catggcTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGTGGCTGGCCACTTCATCGCCGTGTCCg                          <  8:119635/149‑1 (MQ=255)
                                                                                                                                   tccccGGCGGGCATGCCTTGTGGATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGTGGCTGGCCACTTCATCGCCGTGTCCGTAGTCCCt                  >  6:111531/2‑149 (MQ=38)
                                                                                                                                     cccGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGTGGCTGGCCACTTCATCGCCGTGTCCGTAGTCCCtt                 >  8:37913/1‑148 (MQ=255)
                                                                                                                                       cggcggGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTggcg                                                                                   <  6:212350/80‑1 (MQ=255)
                                                                                                                                       cggcggGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTggcg                                                                                   >  5:212350/1‑80 (MQ=255)
                                                                                                                                        ggcggGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGTGGCAGGCCACTTCATCGCCGTGTCCGTAGTCCCTTTCCa             <  2:27344/149‑1 (MQ=255)
                                                                                                                                             gCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGTGGCTGGCCACTTCATCGCCGTGTCCGTAGTCCCTTTCCAGTGc         <  4:63837/148‑1 (MQ=255)
                                                                                                                                             gCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGTGGCTGGCCACTTCATCGCCGTGTCCGTAGTCCCTTTCCAGTGc         <  4:40525/148‑1 (MQ=255)
                                                                                                                                             gCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGTGGCTGGCCACTTCATCGCCGTGTCCGTAGTCCCTTTCCAGTGc         >  3:63837/1‑148 (MQ=255)
                                                                                                                                             gCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGTGGCTGGCCACTTCATCGCCGTGTCCGTAGTCCCTTTCCAGTGc         >  3:40525/1‑148 (MQ=255)
                                                                                                                                                  cacaAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCAcgccg                                                                                         >  2:309696/1‑63 (MQ=255)
                                                                                                                                                  cacaAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCAcgccg                                                                                         <  1:309696/63‑1 (MQ=255)
                                                                                                                                                   acaAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGTGGCTGGCCACTTCATCGCCGTGTCCGTAGTCCCTTTCCAGTGCTtgcatg  >  5:300684/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCACCCGTGACGCCGCCAGGATCAGTTCGCGCAGCAGGCCGGACACGACGATGACCTGGCAGCGTTCGATGTGGGCATCGAGCAGCAAGGTGCGCATGCAGACCACGCCTTTCATGCGGATGGCATGGCTGACCCCGGCGGGCATCCACAAGGCATGCCCGCCGGGGATCACCCAGCTGCCGCCCTCGCTGCTGACCCGCATCACGCCGCTGGCGGCGTAAATCAGCTGGTCTTCGGCGTGGCAGTGGCTGGCCACTTCATCGCCGTGTCCGTAGTCCCTTTCCAGTGCTTGCATG  >  NC_002947/2356527‑2356822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑