Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,372,084 A→C 10.7% intergenic (+36/+76) gdhB → / ← PP_2081 NAD‑specific glutamate dehydrogenase/phosphoenolpyruvate synthase regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,372,0840AC10.7% 117.0 / 5.6 45intergenic (+36/+76)gdhB/PP_2081NAD‑specific glutamate dehydrogenase/phosphoenolpyruvate synthase regulatory protein
Reads supporting (aligned to +/- strand):  ref base A (20/20);  new base C (2/3);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01

CTCGACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAA  >  NC_002947/2371938‑2372226
                                                                                                                                                  |                                                                                                                                              
cTCGACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGAt                                                                                                                                               <  5:251099/148‑1 (MQ=255)
cTCGACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATg                                                                                                                                              <  2:42199/149‑1 (MQ=255)
  cgacgaCCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGATGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGcc                                                                                                                                            >  4:263654/1‑149 (MQ=255)
    acgacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCgg                                                                                                                                          <  6:128963/149‑1 (MQ=255)
      gacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAAGCCCCGGCATCACTGCCgggg                                                                                                                                        <  3:111915/149‑1 (MQ=255)
      gacCTGCGCAATGCCACGGGCACTGACTATGCGAGGTACGCGGTGGCCAACCGCGAGCTGGGCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGGGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAACCCCCGGCATCACTGCCgggg                                                                                                                                        <  1:110309/149‑1 (MQ=255)
       acCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGc                                                                                                                                       >  5:239891/1‑149 (MQ=255)
         cTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCtt                                                                                                                                     >  1:223767/1‑149 (MQ=255)
             gcAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGGGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCtttttt                                                                                                                                 <  6:239891/149‑1 (MQ=255)
                   ccACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTAt                                                                                                                           >  5:119588/1‑149 (MQ=255)
                   ccACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGGGGTGCCGTCCTGAGCCTGGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTAt                                                                                                                           >  8:52627/1‑149 (MQ=255)
                       gggCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCt                                                                                                                       <  1:248811/149‑1 (MQ=255)
                           aCTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTgggc                                                                                                                   >  4:256496/1‑149 (MQ=255)
                             tGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTgggcgg                                                                                                                 >  8:180455/1‑149 (MQ=255)
                             tGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTgggcgg                                                                                                                 >  2:195600/1‑149 (MQ=255)
                                 tATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCgggg                                                                                                                                        <  3:178801/122‑1 (MQ=255)
                                 tATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCgggg                                                                                                                                        >  4:178801/1‑122 (MQ=255)
                                               ggTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAAc                                                                                               <  2:223767/149‑1 (MQ=255)
                                                       aCCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGATGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCt                                                                                       <  3:263654/149‑1 (MQ=255)
                                                        ccGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTa                                                                                      >  5:182954/1‑149 (MQ=255)
                                                           cgAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAg                                                                                   <  6:209484/149‑1 (MQ=255)
                                                               cTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAggggg                                                                               <  1:195600/149‑1 (MQ=255)
                                                                tGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAgggggg                                                                              >  4:77008/1‑149 (MQ=255)
                                                                tGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAgggggg                                                                              <  3:256496/149‑1 (MQ=255)
                                                                        cTGGCCATGAGCGGGCAGGGAGGGGGGGTGCCGTCCTTAGACTTGTAGTGAGGTAAAACAATGCCCCGGAAGTGCTGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCt                                                                      <  8:105919/149‑1 (MQ=38)
                                                                        cTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGggggcgggg                                                                        <  3:188561/147‑1 (MQ=255)
                                                                        cTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGggggcgggg                                                                        >  4:188561/1‑147 (MQ=255)
                                                                        cTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCt                                                                      >  1:278378/1‑149 (MQ=255)
                                                                                      gcaggcagCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGt                                                        <  6:182954/149‑1 (MQ=255)
                                                                                          gcagCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGtctct                                                    >  5:331607/1‑149 (MQ=255)
                                                                                                                ccTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAg                                 >  6:250814/1‑146 (MQ=255)
                                                                                                                    gTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCGACGCCTTTTTCGACCAGGATCTTg                          >  5:141750/1‑149 (MQ=255)
                                                                                                                    gTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTg                          >  5:123306/1‑149 (MQ=255)
                                                                                                                     tAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTgg                         <  6:119588/149‑1 (MQ=255)
                                                                                                                     tAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTGCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTg                          <  7:180455/148‑1 (MQ=255)
                                                                                                                     tAGTGACGTAAAACAACGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTCTCCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTg                          <  2:278378/148‑1 (MQ=255)
                                                                                                                      aGTGAGGTACAACCCCGCCCCGGCAGTGATGCCGGGGATTTTTTTTTTATGCCTGGGCGGGCCATTTCGCGGGTTAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTgg                         <  6:141750/148‑1 (MQ=255)
                                                                                                                          atacaaaaaaaaGCCCCGGCATCACTGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGa                     >  4:29546/5‑148 (MQ=38)
                                                                                                                          aGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGGGGGCCATTTCGCGGGTGAACCCGCGCCGACAGGGGGGGCGGGGCTGTTACTTGACCCGTGTCTCCCCGGCGTTTTCGACCAGGCTGTTGGCGGa                     >  5:196411/1‑148 (MQ=255)
                                                                                                                             tAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAAtc                 >  5:41066/1‑149 (MQ=255)
                                                                                                                             aaaaaaaaaGCCCCGGCATCACTGCCGGGGCTTTGTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAAtc                 >  6:294412/2‑149 (MQ=39)
                                                                                                                                   acaGCCCCGGCCGGGAGCCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCCTTTCGCGGGTGCCCCCGCTCCTCCCGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTcc           <  6:196411/147‑1 (MQ=255)
                                                                                                                                      gCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCAcc        >  7:166519/1‑149 (MQ=255)
                                                                                                                                        cccGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGa      <  6:41066/149‑1 (MQ=255)
                                                                                                                                            gCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGaaaaa  <  6:331607/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CTCGACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAA  >  NC_002947/2371938‑2372226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑