Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,376,709 G→A 7.8% intergenic (+70/‑135) PP_2083 → / → PP_2084 lipase/ribonuclease activity regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,376,7090GA 7.8% 146.5 / 2.6 51intergenic (+70/‑135)PP_2083/PP_2084lipase/ribonuclease activity regulator
Reads supporting (aligned to +/- strand):  ref base G (22/25);  new base A (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01

GTGGAGCGCTGGCTGCACAACCCGCTGCTGCCGGTGCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGGTGTAGCCTTGGCCGATACCCGCTTTCGTTGTGACTGACAGGAGCTTCCCATGCAGCATT  >  NC_002947/2376562‑2376853
                                                                                                                                                   |                                                                                                                                                
gtggAGCGCTGGCTGCACAACCCGCTGCTGCCGGTGCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCtgtg                                                                                                                                                  <  7:56310/148‑1 (MQ=255)
 tggAGCGCTGGCTGCACAACCCGCTGCTGCCGGTGCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCtgtg                                                                                                                                                  <  4:264153/147‑1 (MQ=255)
 tggAGCGCTGGCTGCACAACCCGCTGCTGCCGGTGCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCtgtg                                                                                                                                                  >  3:264153/1‑147 (MQ=255)
      cgcTGGCTGCACAACCCGCTGCTGCCGGTGCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAAc                                                                                                                                           <  1:235503/149‑1 (MQ=255)
      cgcTGGCTGCACAACCCGCTGCTGCCGGTGCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAAc                                                                                                                                           >  2:302582/1‑149 (MQ=255)
          ggctgCACAACCCGCTGCTGCCGGTGCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGt                                                                                                                                       >  5:218434/1‑149 (MQ=255)
          ggctgCACAACCCGCTGCTGCCGGTGCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGt                                                                                                                                       >  2:309619/1‑149 (MQ=255)
                     ccGCTGCTGCCGGTGCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGaa                                                                                                                            >  4:157511/1‑149 (MQ=255)
                       gctgctGCCGGTGCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAgg                                                                                                                          <  5:301370/149‑1 (MQ=255)
                           ctgcCGGTGCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGc                                                                                                                        <  5:232586/147‑1 (MQ=255)
                                 gTGCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTcatg                                                                                                                                   <  6:218185/130‑1 (MQ=255)
                                 gTGCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTcatg                                                                                                                                   >  5:218185/1‑130 (MQ=255)
                                  tGCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAgg                                                                                                               <  1:309619/149‑1 (MQ=255)
                                   gCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAg                                                                                                                                               >  5:78674/1‑116 (MQ=255)
                                   gCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAgg                                                                                                               <  8:278869/148‑1 (MQ=255)
                                   ccATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAg                                                                                                                                               <  6:78674/115‑1 (MQ=255)
                                     aTGCGTCCACTGTAGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATg                                                                                                            <  3:157511/149‑1 (MQ=255)
                                              cTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAAt                                                                                                   <  5:329641/149‑1 (MQ=255)
                                               tGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGaa                                                                                                                            <  5:220877/123‑1 (MQ=255)
                                               tGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAAt                                                                                                   >  7:226615/1‑148 (MQ=255)
                                               tGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGAGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGaa                                                                                                                            >  6:220877/1‑123 (MQ=255)
                                                 tCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGAc                                                                                                >  3:71921/1‑149 (MQ=255)
                                                             cTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCg                                                                                    >  7:199295/1‑149 (MQ=255)
                                                                tgcctgccAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGa                                                                                  <  1:247984/148‑1 (MQ=255)
                                                                  cctgccAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGccc                                                                                           >  4:287447/1‑137 (MQ=255)
                                                                  cctgccAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGccc                                                                                           <  3:287447/137‑1 (MQ=255)
                                                                  cctgccAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGa                                                                               <  8:226615/149‑1 (MQ=255)
                                                                      ccAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTAc                                                                           <  4:71921/149‑1 (MQ=255)
                                                                      ccAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTAc                                                                           <  3:257699/149‑1 (MQ=255)
                                                                       cAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACg                                                                          <  1:302582/149‑1 (MQ=255)
                                                                        aGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGa                                                                         >  3:314291/1‑149 (MQ=255)
                                                                              gTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCga                                                                                                                     >  7:108675/1‑99 (MQ=255)
                                                                              gTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCga                                                                                                                     <  8:108675/99‑1 (MQ=255)
                                                                                        ccTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGgtgt                                                          <  8:250474/148‑1 (MQ=255)
                                                                                         cTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCt                                                                   >  6:137356/1‑138 (MQ=255)
                                                                                         cTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCt                                                                   <  5:137356/138‑1 (MQ=255)
                                                                                              aTGGCCTCTTCGCGGGTGAACCCGCTCCTACACTTGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGGTGTAGCCtt                                                    <  2:308745/148‑1 (MQ=255)
                                                                                                 gCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGGTGTAGCCTTGGcc                                                >  4:294419/1‑149 (MQ=255)
                                                                                                   ctctTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGGTGTAGCCTTGGCCGa                                              >  7:14497/1‑149 (MQ=255)
                                                                                                    tctTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGAt                                                                                 <  5:25248/113‑1 (MQ=255)
                                                                                                    tctTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGAt                                                                                 >  6:25248/1‑113 (MQ=255)
                                                                                                    tctTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGGTGTAGCCTTGGCCGa                                              >  1:68443/1‑148 (MQ=255)
                                                                                                      ttCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGAt                                                                                                             >  7:64992/1‑83 (MQ=255)
                                                                                                      ttCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGAt                                                                                                             <  8:64992/83‑1 (MQ=255)
                                                                                                       tCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGGTGTAGCCTTGGCCGATAcc                                          >  6:116100/1‑149 (MQ=255)
                                                                                                           gggTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGGTGTAGCCTTGGCCGATACCCGCt                                      >  4:100614/1‑149 (MQ=255)
                                                                                                                   ccGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGGTGTAGCCTTGGCCGATACCCGCTTTCGTtgt                              >  2:66886/1‑149 (MQ=255)
                                                                                                                                         ccgTTCCTGCACAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGGTGTAGCCTTGGCCGATACCCGCTTTCGTTGTGACTGACAGGAGc                 >  2:123667/2‑140 (MQ=255)
                                                                                                                                         ccgTTCCTGCACAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGGTGTAGCCTTGGCCGATACCCGCTTTCGTTGTGACTGACAGGAGc                 >  2:118975/2‑140 (MQ=255)
                                                                                                                                         ccgTTCCTGCACAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGGTGTAGCCTTGGCCGATACCCGCTTTCGTTGTGACTGACAGGAGc                 <  1:123667/139‑1 (MQ=255)
                                                                                                                                         ccgTTCCTGCACAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGGTGTAGCCTTGGCCGATACCCGCTTTCGTTGTGACTGACAGGAGc                 <  1:118975/139‑1 (MQ=255)
                                                                                                                                          cgTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGGTGTAGCCTTGGCCGATACCCGCTTTCGTTGTGACTGACAGGAGCTTCCCATgca       <  3:235117/149‑1 (MQ=255)
                                                                                                                                          cgTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGGTGTAGCCTTGGCCGATACCCGCTTTCGTTGTGACTGACAGGAGCTTCCCATgca       <  2:68443/149‑1 (MQ=255)
                                                                                                                                               cTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGGTGTAGCCTTGGCCTATACCCGCTTTCGTTGTGACTGACAGGAGCTTCCCATGCAGCAtt  <  1:66886/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GTGGAGCGCTGGCTGCACAACCCGCTGCTGCCGGTGCATGCGTCCACTGTCGTTGCGGAACCTGTGCCTGCCAGCTGAGTTTGCTGTACCTGTCATGGCCTCTTCGCGGGTGAACCCGCTCCTACACTGGATAACGCGCGTTCCTGTGCAGGAACGGGTCATGCATGGAAGGGCCGAGACAGGATGAATACAAATGACTGCCCGGTCCCGGATGACTACGACAGCCTCCAAGGTGTAGCCTTGGCCGATACCCGCTTTCGTTGTGACTGACAGGAGCTTCCCATGCAGCATT  >  NC_002947/2376562‑2376853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑